EMAGE mouse gene expression spatial database

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EMAGE:31696

Tpbg trophoblast glycoprotein ( MGI:1341264)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000218_01	euxassay_000218_02	euxassay_000218_03	euxassay_000218_04	euxassay_000218_05

euxassay_000218_06	euxassay_000218_07	euxassay_000218_08	euxassay_000218_09	euxassay_000218_10

euxassay_000218_11	euxassay_000218_12	euxassay_000218_13	euxassay_000218_14	euxassay_000218_15

euxassay_000218_16	euxassay_000218_17	euxassay_000218_18	euxassay_000218_19	euxassay_000218_20

euxassay_000218_21	euxassay_000218_22	euxassay_000218_23	euxassay_000218_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thalamus

weak

single cell

weak expression: see section 19

metencephalon rest of alar plate mantle layer

moderate

single cell

moderate expression: see section 05 07 weak expression: see section 19 20

pineal primordium

moderate

homogeneous

moderate expression: see section 12 13

metanephros

moderate

homogeneous

moderate expression: see section 20 21 22

ureter

moderate

homogeneous

moderate expression: see section 14 15 19

spinal cord roof plate

strong

homogeneous

strong expression: see section 14 17 18 19 moderate expression: see section 15 16

thalamus mantle layer

moderate

single cell

moderate expression: see section 06 weak expression: see section 07 18

medulla oblongata lateral wall

strong

single cell

strong expression: see section 07 08 moderate expression: see section 11 12 13 14 15 16 17 18 weak expression: see section 09 10

bladder

moderate

homogeneous

moderate expression: see section 15

cerebral cortex ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 15 16 17 18 19 20 moderate expression: see section 01 04 21 22

epithalamus ventricular layer

moderate

homogeneous

moderate expression: see section 11 13 14

spinal cord floor plate

weak

homogeneous

weak expression: see section 14 15 16 17

renal cortex

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 21 22 weak expression: see section 23 24

kidney pelvis

moderate

homogeneous

moderate expression: see section 12 13

hypothalamus ventricular layer

strong

regional

strong expression: see section 12 moderate expression: see section 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T413
Entity Detected:	Tpbg, trophoblast glycoprotein ( MGI:1341264)
Sequence:	sense strand is shown >T413 TAAAAAAGTGGATGCATAACATCAGAGATGCCTGCAGGGATCACATGGAAGGGTATCATTACAGATACGA AATCAATGCGGACCCCAGATTAACAAATCTTAGTTCCAACTCGGATGTCTGAGAAACAGGACCCATCAAG GAGGACTCTGCATGAGGTGTAGACTTAAGGGTTTTTTTTTTCCCCTTTACTAGGCTTGCTCCACTTCCAC ACTTCACCGTAGACACTATTGACTTTGATCCTTGTTATGTAAAATCCCGGTGTGTTCTGTTAATGTAAGG ACATGAACAACTGCACATGTTTATTTTCCCTATTCCTTTCGGTACTCCTCGATGCCTGCGGAGGGATTGT TTTTTTTTCTCTCTCTCTCAAGTCGCAGCATGGACTTGGATTAGTTCAGTTCAAGGTGTAGCACAGATAA CATTCAACGGAAGCTGCCTCAGTTTTTTTTTTTTTTTGAGAAAAGTACTTTATTCATAAATATGAGTTTC ATCCTCACCTACC
Notes:	The probe template was PCR amplified from IMAGE:3157645 using vector specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3157645 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000218 same experiment
	EMAGE:30703 same embryo
	EMAGE:29443 same embryo
	EMAGE:29445 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source