EMAGE mouse gene expression spatial database

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EMAGE:31702

Rab27a ( MGI:1861441)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004818_01	euxassay_004818_02	euxassay_004818_03	euxassay_004818_04	euxassay_004818_05

euxassay_004818_06	euxassay_004818_07	euxassay_004818_08	euxassay_004818_09	euxassay_004818_10

euxassay_004818_11	euxassay_004818_12	euxassay_004818_13	euxassay_004818_14	euxassay_004818_15

euxassay_004818_16	euxassay_004818_17	euxassay_004818_18	euxassay_004818_19	euxassay_004818_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

adenohypophysis

strong

regional

strong expression: see section 06 07 08 09 10

thymus primordium

strong

homogeneous

strong expression: see section 06 07 08 09

upper lip

weak

regional

weak expression: see section 06

cervical ganglion

strong

regional

strong expression: see section 10 moderate expression: see section 04

adrenal medulla

moderate

regional

moderate expression: see section 04 09 10

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 moderate expression: see section 04

thoracic ganglion

strong

regional

strong expression: see section 07 08 moderate expression: see section 06

lower lip

moderate

regional

moderate expression: see section 07 weak expression: see section 06

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6513
Entity Detected:	Rab27a, ( MGI:1861441)
Sequence:	sense strand is shown >T6513 AAGCACCCAAAAAAGGCCAGTCGCACGGGGTCCGGAGCTCCATTTGGAAGGGAGTACCTAAGGGATAGAG CACAGCGAGGACGCGGCACGTTGGGAATCTAGCACTGCAGGGACGCAACACCGCAGGGACAGCACCGGAC AGAGCACCAAACCGGGTGAGGCACAGAGTCCTCCATCATGTCGGATGGAGATTACGATTACCTCATCAAG TTCTTGGCCTTGGGAGACTCTGGGGTAGGGAAGACGAGTGTACTCTACCAGTACACTGATGGCAAGTTCA ACTCCAAATTCATCACCACAGTGGGCATTGATTTCAGGGAAAAGAGAGTGGTGTACAGAGCCAATGGGCC AGATGGAGCTGTGGGCCGAGGCCAGAGAATCCACCTGCAGTTATGGGACACGGCGGGGCAGGAGAGGTTT CGTAGCTTAACCACTGCATTCTTCAGGGACGCTATGGGTTTCCTGCTTCTGTTCGACCTGACAAATGAGC AAAGTTTCCTCAATGTCCGAAACTGGATAAGCCAGCTACAGATGCACGCGTACTGTGAAAACCCAGATAT AGTGCTGTGTGGAAATAAGAGTGACCTGGAAGACCAGAGGGCAGTGAAAGAGGAGGAGGCCCGGGAGCTT GCCGAGAAGTACGGAATCCCCTATTTTGAAACCAGTGCTGCCAACGGGACAAACATAAGCCACGCGATTG AGATGCTCCTGGACCTGATCATGAAGCGGATGGAGCGGTGTGTGGACAAGTCCTGGATTCCGGAGGGAGT GGTACGGTCCAACGGCCATACCTCTGCGGATCAGCTAAGTGAGGAGAAGGAGAAGGGGTTGTGTGGCTGT TGAGACGCCAAGCGAGCCCCGTGGCAGCAGCGCATGACCCCTGGTTGCCGCCGGCTCCGCTGTAGAGACC AAGCCGGCAGCTCGATGAGATGAGCAAACAGCCCCTCCTATGCCAGGTGCCTCTCCTCGCCTCCAGGAGG GTCACCTCGGGCATCCATCTGTAACGCGCTGCAGCTTTGTATGATTTAGTCCATGAATCGGAAGAAATAC TTCACAGAGCCAAAAGTGTCTTATAAAAGCCCACGGTGGCGGGTCATTTAGGGATCCAAGATTTCAGCCA TGAAAGATTTCATCTGTTATGTAGAACGCTGATGGTCCTGTCGCCTGCCTTAACACAGTCGCCTGCCTCA ACACAGGACAGCCCTAGAGTCTCATTGAAGACATTAAGATCTTTGAGCAAGGAAAAGCTAGCACGGTCTT GAGAATCCAGATTTCAAAATCCATACTGTAGACTATCACTCTCAAACACATGAATGTCAATAAGTGTGTA CACACAGATCAATGTCTTTCAGCATTGCTTTTTAACATAGCCTTCCCTTTGACATGAGCATAAACAGATG GCGGCCATACCTATAGACTCTGAATAGATCGGATTGACTCACTGTGAGTTTGGGGGTGGGGGGAAGGGTT TATGGAGCAGGGGTGGGTGAAGTAGGGATCAGTCAGATTGTCTGCTGTTAGCCGAGGGGTCATGAAGACT CTTGTGTCTATCCATGGTCTGTTGGGTTCAGTGCACCTCTGAACATCGCTCATGCTTCTACAGTATGTAT GTTTGCATGTATGTATGTATGGTCTCTTCATATGTACGTATGTTTGGTCTGGCACCTCAGGATCTGCTTT CACTTGTCTGGAGACTGCCAATCTGACTTTTCCCATCAAGTTTTGGTGGTGGTGGTGTTTCCCAGTTTGT TTTCTCCCCCCCCCCTTCCCCCATTAATGTAACTGAAGGAATCTCTTTTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3590089 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3590089 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004818 same experiment
	EMAGE:31617 same embryo
	EMAGE:31692 same embryo
	EMAGE:29645 same embryo
	EMAGE:31700 same embryo
	EMAGE:31688 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source