EMAGE mouse gene expression spatial database

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EMAGE:31709

Hdac10 histone deacetylase 10 ( MGI:2158340)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_009262_01	euxassay_009262_02	euxassay_009262_03	euxassay_009262_04	euxassay_009262_05

euxassay_009262_06	euxassay_009262_07	euxassay_009262_08	euxassay_009262_09	euxassay_009262_10

euxassay_009262_11	euxassay_009262_12	euxassay_009262_13	euxassay_009262_14	euxassay_009262_15

euxassay_009262_16	euxassay_009262_17	euxassay_009262_18	euxassay_009262_19	euxassay_009262_20

euxassay_009262_21	euxassay_009262_22	euxassay_009262_23	euxassay_009262_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

single cell

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

neural retina

weak

regional

weak expression: see section 03 04 05 24

trigeminal v ganglion

moderate

single cell

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21

cervical ganglion

moderate

regional

moderate expression: see section 07 08 15

vestibulocochlear viii ganglion

moderate

single cell

moderate expression: see section 05 06 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 07 13

spinal cord

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13

brain

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 05 06 16 17

thoracic ganglion

moderate

regional

moderate expression: see section 10 11 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T570
Entity Detected:	Hdac10, histone deacetylase 10 ( MGI:2158340)
Sequence:	sense strand is shown >T570 TCCTCGAGNCTGTTGGCCTACTGGCAGTTTCCAGCGGTTCTCTTCTGACATCGGGCGGGCTTGAGCCCGG ACCTGGGCCTGGGGTGGTTTCCTCTTGCCCAGGTCCAGCGTGCACAACCCAGTTCGTCCCGAGTGAGGAT TGTTTACGTGCTTAGTTAGGTGAGACCGGGCAGTGCAATTACCTGCCGGCAGAGGGCGTGTTGCGTTAGA GCGGATCAAAGTGACCACGGTGTAGCTATGGGCACAGCACTTGTGTACCACGAGGACATGACAGCCACTC GACTGCTCTGGGATGACCCCGAGTGCGAAATTGAGTGCCCAGAGCGCCTGACAGCTGCCCTGGATGGCCT GCGGCAGCGTGGCCTGGAAGAAAGGTGCCTGTGTTTGTCAGCTTGTGAGGCATCAGAGGAAGAGTTGGGC TTGGTGCACAGCCCGGAATATATAGCCCTGGTGCAGAAGACCCAGACCCTGGATAAAGAGGAGCTCCACG CACTGTCTAAGCAGTACAATGCTGTCTACTTTCACCCGGACACCTTTCACTGTGCCCGGCTGGCAGC
Notes:	The probe template was PCR amplified from IMAGE:1884975 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1884975 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009262 same experiment
	EMAGE:31608 same embryo
	EMAGE:30441 same embryo
	EMAGE:30452 same embryo
	EMAGE:30440 same embryo
	EMAGE:30433 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source