EMAGE mouse gene expression spatial database

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EMAGE:31713

Spc25 SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) ( MGI:1913692)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000451_11	euxassay_000451_01	euxassay_000451_02	euxassay_000451_03	euxassay_000451_04

euxassay_000451_05	euxassay_000451_06	euxassay_000451_07	euxassay_000451_08	euxassay_000451_09

euxassay_000451_10	euxassay_000451_12	euxassay_000451_13	euxassay_000451_14	euxassay_000451_15

euxassay_000451_16	euxassay_000451_17	euxassay_000451_18	euxassay_000451_19	euxassay_000451_20

euxassay_000451_21	euxassay_000451_22	euxassay_000451_23	euxassay_000451_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 24

midbrain ventricular layer

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 14 15 16 17 18 weak expression: see section 24

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 24

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 08 09 14 15

ventral grey horn

moderate

regional

moderate expression: see section 12 14 24

medulla oblongata

moderate

regional

moderate expression: see section 14 24

pons mantle layer

weak

regional

weak expression: see section 09 10 12

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 10 11 12 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3786
Entity Detected:	Spc25, SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) ( MGI:1913692)
Sequence:	sense strand is shown >T3786 GGCAGCAGACCCGGGAGCAGCAGACGGAAGAGCGGCGGGCTGGTTCCTGCCGGACAGGTCCAGTCTGGTG GAGACCGCGGCGTTCCTGGCCGCTCAGTGTCTTAAAACATAATGGGTGAAGACGAATTGGCACTCTTAAA TCAAAGCATCAATGAATTTGGGGATAAGTTCAGAAACAGACTCGATGATAATCATAGTCAAGTGCTGGGA CTTAGAGACGCCTTCAAGGACTCCATGAAAGCGTTTTCAGAAAAGATGTCTTTGAAATTAAAGGAAGAAG AGAGGATGACTGAGATGATTCTGGAGTATAAAAACCAGCTCTGTAAGCAGAATAAGCTCATTCAAGAAAA GAAAGAGAATGTGTTGAAGATGATTGCTGAAGTAAAAGGCAAGGAGCAAGAGTCGGAAGAGCTGACTGCT AAAATCCAGGAGCTCAAGGAAGAGTACGCTAGGAAGAGGGAAACCATTTCCACTGCTAACAAAGCTAATG AAGAGAGATTGAAAGGACTGCAGAAATCA
Notes:	The probe template was PCR amplified from IMAGE:391122 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:391122 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000451 same experiment
	EMAGE:30052 same embryo
	EMAGE:29720 same embryo
	EMAGE:29202 same embryo
	EMAGE:29215 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source