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EMAGE:31715

Prmt1 protein arginine N-methyltransferase 1 ( MGI:107846)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31715 EMAGE:31715 EMAGE:31715 EMAGE:31715 EMAGE:31715
euxassay_000210_01 euxassay_000210_02 euxassay_000210_03 euxassay_000210_04 euxassay_000210_05
EMAGE:31715 EMAGE:31715 EMAGE:31715 EMAGE:31715 EMAGE:31715
euxassay_000210_06 euxassay_000210_07 euxassay_000210_08 euxassay_000210_09 euxassay_000210_10
EMAGE:31715 EMAGE:31715 EMAGE:31715 EMAGE:31715 EMAGE:31715
euxassay_000210_11 euxassay_000210_12 euxassay_000210_13 euxassay_000210_14 euxassay_000210_15
EMAGE:31715 EMAGE:31715 EMAGE:31715 EMAGE:31715 EMAGE:31715
euxassay_000210_16 euxassay_000210_17 euxassay_000210_18 euxassay_000210_19 euxassay_000210_20
EMAGE:31715 EMAGE:31715 EMAGE:31715
euxassay_000210_21 euxassay_000210_22 euxassay_000210_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
midgut loop lumen
weak weak
regionalweak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18
tail dorsal root ganglion
weak weak
regionalweak expression: see section 11 13 18 19 20
right lung
moderate moderate
regionalmoderate expression: see section 14 15 16 17 18 19 20 21 22 23
chondrocranium
moderate moderate
regionalmoderate expression: see section 03 04 05 21 22 23 weak expression: see section 06 20
not examined not examined
spottednot examined expression: see section 02
left lung
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13
pancreas
weak weak
regionalweak expression: see section 10 11 12 13
submandibular gland primordium
strong strong
regionalstrong expression: see section 08 09 11 moderate expression: see section 10 17 18 19 20
not examined not examined
spottednot examined expression: see section 02
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
thymus primordium
weak weak
regionalweak expression: see section 13 14 15 16 17
not examined not examined
spottednot examined expression: see section 02
renal cortex
moderate moderate
homogeneousmoderate expression: see section 09 10 11 12 19 20 21 22
vibrissa
moderate moderate
regionalmoderate expression: see section 06 07 08 19 20 weak expression: see section 03 04 05 21 22
foot skin
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 21 22 23
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 11 12 15 16
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 11 12 15 16
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T170
Entity Detected:Prmt1, protein arginine N-methyltransferase 1 ( MGI:107846)
Sequence:sense strand is shown

>T170
GGACTACATAGTGAGACAGTTTGAAAAGAGAGACAGGGTGCCTGCCCTTGGACCAGAGTCCTCTGTGGGT
GTCTGGAACCATCTGGGCCAGCCCTCCTCGTGACTGGCATGCCATTCCTGTGGGTGCAGTGGTGACCATC
ATCAAGGGCAAGGTGGAGGAGGTGGAGCTGCCCGTGGAGAAGGTGGACATCATCATCAGCGAGTGGATGG
GTTACTGCCTCTTCTACGAGTCCATGCTCAACACCGTGCTGCATGCTCGGGACAAGTGGCTGGCACCCGA
TGGCCTCATCTTCCCAGACCGGGCCACCTTGTATGTGACAGCCATTGAGGACCGACAATATAAAGACTAC
AAGATCCACTGGTGGGAGAACGTGTATGGCTTTGATATGTCCTGCATTAAAGACGTGGCCATCAAGGAGC
CCCTGGTGGACGTGGTGGACCCAAAGCAGCTGGTCACCAATGCCTGCCTCATAAAGGAGGTGGACATCT
Notes:The probe template was PCR amplified from IMAGE:2645661 using vector specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2645661 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000210 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS