EMAGE mouse gene expression spatial database

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EMAGE:31719

Arhgap9 ( MGI:2143764)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001996_01	euxassay_001996_02	euxassay_001996_03	euxassay_001996_04	euxassay_001996_05

euxassay_001996_06	euxassay_001996_07	euxassay_001996_08	euxassay_001996_09	euxassay_001996_10

euxassay_001996_11	euxassay_001996_12	euxassay_001996_13	euxassay_001996_14	euxassay_001996_15

euxassay_001996_16	euxassay_001996_17	euxassay_001996_18	euxassay_001996_19	euxassay_001996_20

euxassay_001996_21	euxassay_001996_22	euxassay_001996_23	euxassay_001996_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 07 08 09 10 11 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4593
Entity Detected:	Arhgap9, ( MGI:2143764)
Sequence:	sense strand is shown >T4593 GAAGAGCAAAGACTGTGAGGGGAAGAATTTTTTAAAAAACAGCCAGGGACTGGGGTTTCTACCCATGTAC CAGGGGCCCCCCCGGGCCCGGAGCTGACCAAACCGGGCTCCATGGAGCAGACAGAGACTTTGACTAGGGA CAGCAGTGCCCTACAGCCTCAGGAATGGCTCGATCCACAGGGTTTACCAAGCCTCAGCCAAAGCGTCCCA TGGCTTGCTCTCTCTGAACAGCCGGGAGCCTTGAGGCCTTGTTCAGCTCTGCCACAGCTCCTGGATGACC CCCACGTTGAAGAAAGGTCAGGCCTGCTCAACATGACCAAGATTGCTCAAGGAGGGCGCAAACTCAGGAA GAACTGGGGCCCTGCTTGGGTGGTGTTAACTGGTAGCAGCCTTGTGTTTTACCGAGAGCGGCCACCGCAG TCTGCATCCCTCCAAGGCTGGGCGCGGGCTGGGAGCCGGCCGGAGAGTAGCGTGGACCTGCGCGGGGCGG CCCTGGCCAGCGGGCGCCAGCTGTCCAGCCGCCGGAACGTCCTGCACATCCGCACGGTCCCGGGACATGA GTTCCTGCTGCAGTCGGACGAGGAGACCGAGCTGCGAGACTGGCACCGCGCGCTGCGGACTGTCATCGAG CGGCTGGATCGGGAGAACCCCCTGGAGCTGCGTCTGTCGGGCTCCGGACCCGCCGAGCTGGCCGAGCTGA GCGCTGGTGAGGATGACGAATTGGAATCGGAACCGGTGTCCAAGTCCCTGATGCGGCTTGGCAGCCGCAG AACTTCCAGTCGGTGTGCAGAGGGCACTGACCAGAAGAACCGCGTGCGGAACAAGTTAAAGCGGCTAATC GCCAAGAGACCAACCTTGCAGAGCCTACAAGAGCGGGGTCTGTTCCGAGACCAGGTTTTTGGCTGCCAGT TGGAGTCGCTGTGCCAACGGGAAGGGGACACCGTGCCCAGCTTTGTCCGGCTTTGTGTTGAGGCCGTGGA TAAAAAAGGTCTAGACGTGGATGGTATTTACCGGGTGAGTGGGAACCTGGCCGTGGTCCAGAAACTCCGC TTCCTGGTAGACAGAGAACGGGCAGTGACCTCTGATGGGAGATACATGTTCCCAGAACAGGCAGGACAAG AAGGCAAATTAGATTTAGACAGTGCCGAATGGGATGATATTCACGTGGTCACCGGAGCTTTGAAGCTTTT TTTCCGGGAGCTGCCACAGCCTCTGGTGCCTGCATTGTTGCTGCCTGACTTTCGTGATGCCCTTGAACTT TCTGAGCCAGAGCAGTGCCTCTCCAAAATCCAGAAATTAATAGACTCGCTGCCGAGGCCCAACCATGACA CTCTGAAGTACATCCTGGAGCATCTGTGCAGGGTGATAGCACACTCGGATAAGAACCGAATGACCGCCCA CAACCTGGGAATTGTGTTTGGACCGACACTGTTTCGACCTGAGCAGGAGGCCTCTGACATGGCGGCCCAC GTATTCTACCCCGGGCAGCTGGTCCAGTTGATGCTCAACAACTTTGCCAGCCTCTTCACTTGACCCAGGC GAGGAAGAGGAAGGCGCTTCCTGTGATTTGTCAGCTAGCCTTTGGAGAGGGCTGGCGGGTTTTCTGTTCT GAAGATACAATGTCCTCCCAAGGGACACCCTTCATTTCCCGCTCCAGTCTCACTTGAGCTCCTGGCTAAG AGGGGTTGCTGAAGGGGAAGGTATAGGAGCCCGCTTCTGCTCTCCCCAACATGACAGGTGATGCTTGTTC TCAGTATTCCTTTATTGTCAGGAAACTGTGTCATTATTAAAGTTTGTTCTTTCCTGGAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4987970 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4987970 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001996 same experiment
	EMAGE:31462 same embryo
	EMAGE:29892 same embryo
	EMAGE:29891 same embryo
	EMAGE:31862 same embryo
	EMAGE:31459 same embryo
	EMAGE:31863 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source