EMAGE mouse gene expression spatial database

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EMAGE:31725

Bcas2 ( MGI:1915433)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001997_01	euxassay_001997_02	euxassay_001997_03	euxassay_001997_04	euxassay_001997_05

euxassay_001997_06	euxassay_001997_07	euxassay_001997_08	euxassay_001997_09	euxassay_001997_10

euxassay_001997_11	euxassay_001997_12	euxassay_001997_13	euxassay_001997_14	euxassay_001997_15

euxassay_001997_16	euxassay_001997_17	euxassay_001997_18	euxassay_001997_19	euxassay_001997_20

euxassay_001997_21	euxassay_001997_22	euxassay_001997_23	euxassay_001997_24	euxassay_001997_25

euxassay_001997_26

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 17 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 22 weak expression: see section 04 05 06 07 08 18 19 20 21

cervical ganglion

weak

regional

weak expression: see section 07 08 16

cervico-thoracic ganglion

weak

regional

weak expression: see section 08 15

spinal cord

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4576
Entity Detected:	Bcas2, ( MGI:1915433)
Sequence:	sense strand is shown >T4576 CCACGCGTCCGGCCAGAAGGCGCTCTTGAAGCCTAGTGCCGCGCGATGGCGGGCACGGGCTTGGTAGCCG GAGAGGTGGTGGTAGATGCGCTACCATATTTTGACCAAGGCTATGAGGCACCCGGCGTGCGGGAAGCGGC GGCGGCCCTGGTGGAGGAGGAGACGCGCAGATACCGACCTACCAAGAACTACCTGAGCTACCTGACGGCC CCGGATTATTCTGCCTTTGAAACAGACATAATGAGAAATGAATTTGAAAGACTCGCTGCTCGACAACCGA TTGAATTACTCAGCATGAAACGATATGAACTTCCAGCCCCTTCCTCAGGTCAAAAAAATGACATTACTGC ATGGCAAGAATGTGTAAACAATTCTATGGCTCAGTTGGAGCACCAGGCGGTCCGGATCGAGAATCTGGAG CTGATGTCACAGCATGGATGCAATGCCTGGAAGGTGTACAATGAAAATCTTGTTCATATGATTGAACATG CACAGAAAGAGCTTCAGAAGTTAAGGAAACATATTCAAGATTTGAACTGGCAGCGAAAGAACATGCAGCT TACAGCTGGATCTAAGCTGAGAGAAATGGAGTCAAACTGGGTGTCGCTGGTGAGTAAGAACTATGAGATT GAGCGGACGATTGTCCAGCTGGAGAACGAGATCTATCAGATCAAGCAGCAGCACGGGGAGGCCAACAAGG AAAACATCCGCCAAGACTTCTAATGACTCAGGCGACGTGGGCTCCGCAGACTCAGCGGGGACTTTCTCGG AGCGACTGTGGTGGTTACATGGTTAGAGACCATGGACTATGTAAACTGTTGTATTGCTTCTGTGTGAGTG TCTTAGCAAAATAAAGTTGTCGACATGTTGCTGTTTTTATGCTATAAAAACCTGGTTTTCAGAATGAGTA TGACTTTAGAATAATTTTGACATATTTTGTAGCAGAATTTTTTTTTTTAAGGCTTCAAATTTTGTAATGA AGCTCCTTTATTTTTAAGGCATCCAACATGTTCTGGAAGCTGGAAACCTTGAATTTGTGTTTTTTGTTGA ATAAAGTTACAGTTCTAAGCATTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4984289 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4984289 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001997 same experiment
	EMAGE:29890 same embryo
	EMAGE:31476 same embryo
	EMAGE:31441 same embryo
	EMAGE:31865 same embryo
	EMAGE:31204 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source