EMAGE mouse gene expression spatial database

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EMAGE:31735

Arl5a ADP-ribosylation factor-like 5A ( MGI:1922673)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002854_01	euxassay_002854_02	euxassay_002854_03	euxassay_002854_04	euxassay_002854_05

euxassay_002854_06	euxassay_002854_07	euxassay_002854_08	euxassay_002854_09	euxassay_002854_10

euxassay_002854_11	euxassay_002854_12	euxassay_002854_13	euxassay_002854_14	euxassay_002854_15

euxassay_002854_16	euxassay_002854_17	euxassay_002854_18	euxassay_002854_19	euxassay_002854_20

euxassay_002854_21	euxassay_002854_22	euxassay_002854_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

weak

regional

weak expression: see section 06 07 08 09 10 14 15 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 02 03 18

medulla oblongata basal plate

moderate

regional

moderate expression: see section 07 14 weak expression: see section 06 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 01 02 03 04 05 06 15 16 17 18 20 weak expression: see section 19

vibrissa

moderate

regional

moderate expression: see section 01 02 03 16 17 18

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05 06 15 16 17

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 16 17 weak expression: see section 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1579
Entity Detected:	Arl5a, ADP-ribosylation factor-like 5A ( MGI:1922673)
Sequence:	sense strand is shown >T1579 CAGAAATCTCCCAGTTTTTGAAGCTTACTTCTATAAAAGACCACCAGTGGCATATTCAAGCATGTTGCGC TCTTACTGGCGAGGGGTTATGCCAAGGACTTGAATGGATGATGTCACGGCTGAAGATCAGATGATCTCTT CTGACCCCTCCTCACAGACTCTGTATAAAGAAAGTGCTGGACTTTTCCTGAAAGCTGCAAAAAAATGGTT TAGATATATTTATAATAAACTGACTTAAGAGTTTTCTATAAGAAGAAAATTAAGACCACTTATTTGAAAA CAAAGATGAAATGTCACCACCCGTTTGCGCTCTCATTCCTTCCAAGCCTGTGACTGGTATTTTCTCATGA TGAATCCTCTCAGGACATGGCAGAGCTTGGCGAGTACAAAGGGAGAGGAAGACATTTGAACTTTAGAACT TTGTTTCAGCGTGACTCTCCCACCCTGCCCCAAGTTGAATGTTCCCTTCACATTGAATCAAATACAAATC AGCACAGATACTGTTTCCAGTTTTTAAAAA
Notes:	The probe template was PCR amplified from IMAGE:934823 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:934823 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002854 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source