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EMAGE:31750

Uchl1 ubiquitin carboxy-terminal hydrolase L1 ( MGI:103149)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31750 EMAGE:31750 EMAGE:31750 EMAGE:31750 EMAGE:31750
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euxassay_007064_11 euxassay_007064_12 euxassay_007064_13 euxassay_007064_14 euxassay_007064_15
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
neural retina
strong strong
regionalstrong expression: see section 01 02 03 04 22 23
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 06 07 08 09 10 17 18 19
stomach
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 11 12 15 16 17 not examined expression: see section 13
foot mesenchyme
moderate moderate
regionalmoderate expression: see section 06 07 08 21 22 23
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 07 08 19
trigeminal v nerve
strong strong
regionalstrong expression: see section 10 17
thoracic ganglion
strong strong
regionalstrong expression: see section 13 15
testis
strong strong
regionalstrong expression: see section 07 08 20 21 22
tongue muscle
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 weak expression: see section 16
midgut
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20
bladder
strong strong
regionalstrong expression: see section 13 14 15
dorsal root ganglion
strong strong
regionalstrong expression: see section 08 09 10 11 12 16 17 18 19
facial vii ganglion
strong strong
regionalstrong expression: see section 04 05 06 07 19 20 21
vagus x ganglion
strong strong
regionalstrong expression: see section 09 18
vomeronasal organ
strong strong
regionalstrong expression: see section 12 13 15
trigeminal v ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 15 16 17 18 19
hindgut
strong strong
regionalstrong expression: see section 14 15 16 17 18
cervical ganglion
strong strong
regionalstrong expression: see section 10 17
adrenal medulla
strong strong
regionalstrong expression: see section 09 10 18 19
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 12 13 16 17
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 12 13 16 17
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4295
Entity Detected:Uchl1, ubiquitin carboxy-terminal hydrolase L1 ( MGI:103149)
Sequence:sense strand is shown

>T4295
CGGACGCGTGGGGTCTGCAGGTGCCATCCGCGAAGATGCAGCTGAAGCCGATGGAGATTAACCCCGAGAT
GCTGAACAAAGTGTTGGCCAAGCTGGGGGTCGCCGGCCAGTGGCGCTTCGCCGACGTGCTAGGGCTGGAG
GAGGAGACTCTGGGCTCAGTGCCATCCCCTGCCTGCGCCCTGCTGCTCCTGTTTCCCCTCACGGCCCAGC
ATGAAAACTTCAGGAAAAAGCAAATTGAGGAACTGAAGGGACAGGAAGTTAGCCCTAAAGTTTACTTCAT
GAAGCAGACCATCGGAAACTCCTGTGGTACCATCGGGTTGATCCACGCAGTGGCCAACAACCAAGACAAG
CTGGAATTTGAGGATGGATCCGTCCTGAAACAGTTTCTGTCTGAAACGGAGAAGCTGTCCCCCGAAGATA
GAGCCAAGTGTTTCGAGAAGAACGAGGCCATCCAGGCGGCCCATGACTCCGTGGCCCAGGAGGGCCAGTG
TCGGGTAGATGACAAAGTGAATTTCCATTTTATTCTGTTCAACAACGTGGACGGCCATCTGTACGAGCTC
GATGGGCGAATGCCCTTTCCAGTGAACCATGGCGCCAGCTCAGAGGACTCTCTGCTGCAGGATGCTGCCA
AGGTCTGCAGAGAATTCACTGAGCGCGAGCAGGGGGAGGTCCGCTTCTCTGCCGTGGCTCTCTGCAAAGC
AGCTTAAGTCTGGGGAGAGAGAACCAGCCGATCCCCCCTTCCCTGGGCAGGTGCGCGCGGCCCGCCCTTG
GTTTGCAGCTTTAGCACTTAGAACCACAGCTGTCTTCTTGCGTTCTACAGCCCCATCCCCTCCACCCCAC
CCAGGCCACCAGGGGGCTCTGTCACAGCCACACCAGGCTGAGCACTTTCCCTCCTGTGTGTCTCGTACCT
TGCTCTCTACGGTCTCTTTGGTTTCTGTCTGTAAGTTACGGCCCTGGATGTGGTTTGTCTAGTCCTTAAG
AGGAAGAATAAAACTTTGCTGGTGAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4506229 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4506229 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_007064 same experiment
 EMAGE:30160 same embryo
 EMAGE:31849 same embryo
 EMAGE:29579 same embryo
 EMAGE:31534 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS