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EMAGE:31761

Cdh7 ( MGI:2442792)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31761 EMAGE:31761 EMAGE:31761 EMAGE:31761 EMAGE:31761
euxassay_009004_01 euxassay_009004_02 euxassay_009004_03 euxassay_009004_04 euxassay_009004_05
EMAGE:31761 EMAGE:31761 EMAGE:31761 EMAGE:31761 EMAGE:31761
euxassay_009004_06 euxassay_009004_07 euxassay_009004_08 euxassay_009004_09 euxassay_009004_10
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euxassay_009004_11 euxassay_009004_12 euxassay_009004_13 euxassay_009004_14 euxassay_009004_15
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euxassay_009004_16 euxassay_009004_17 euxassay_009004_18 euxassay_009004_19 euxassay_009004_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
medulla oblongata basal plate mantle layer
weak weak
regionalweak expression: see section 05 06 07 08 09 10 12
ventral grey horn
strong strong
regionalstrong expression: see section 08 12 moderate expression: see section 07 11 weak expression: see section 06 09 10
olfactory cortex marginal layer
moderate moderate
regionalmoderate expression: see section 10 11 15 16 weak expression: see section 12 17
pons mantle layer
weak weak
regionalweak expression: see section 04 05 06 07 08 09 12 15 16 17
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36140
Entity Detected:Cdh7, ( MGI:2442792)
Sequence:sense strand is shown

>T36140
AGAGGCTACGTGGCCATTACTATACTGGATATTAACGACAACGCACCTGAGTTCGCCATGGACTATGAAA
CCACTGTCTGTGAAAACGCTCAGCCAGGGCAGGTTATCCAGAAAATCAGTGCAGTAGATAAAGATGAGCC
ATCCAATGGACACCAGTTTTACTTCAGCTTAACAACAGACATGACAAATAACCACAACTTTTCATTGAAA
GATAACAAAGACAATACAGCCTCAATACTCACGAGAAGAAATGGCTTCCGAAGACAGGAACAATCAGTTT
ATTATCTGCCCATTTTCATTGTGGACAGTGGATCTCCTTCACTAAGCAGTACCAACACACTTACCATCCG
TGTATGTGACTGTGATGCTGATGGCATAGCCCAGACCTGCAATGCAGAGGCCTATGTTCTACCTGCTGGC
CTCAGCACTGGGGCCTTGATAGCAATACTGGCCTGTGTCCTGACACTATTGGTGCTGATCCTCCTCATTG
TCACTATGAGAAGACGAAAAAAAGAGCCTCTCATTTTTGATGAGGAGAGGGATATCAGAGAAAACATAGT
CAGATATGATGATGAAGGCGGGGGAGAAGAAGACACAGAAGCCTTTGACATGGCTGCGCTGAGAAACCTC
AATGCCATAAGAGACAGCAAAACTCGGAGAGATGTGACTCCTGAAATTCAGTTCTTGAGTCGACCGACTT
TTAAAAACATCCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 93324. Forward Primer - name:093324_F_cDNA_Cdh7, sequence:AGAGGCTACGTGGCCATTACTA; Reverse Primer - name:093324_N_SP6_cDNA_Cdh7, sequence:GGGATGTTTTTAAAAGTCGGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_009004 same experiment
 EMAGE:31759 same embryo
 EMAGE:31714 same embryo
 EMAGE:30553 same embryo
 EMAGE:31751 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS