Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31773

Cds1 CDP-diacylglycerol synthase 1 ( MGI:1921846)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31773 EMAGE:31773 EMAGE:31773 EMAGE:31773 EMAGE:31773
euxassay_002822_01 euxassay_002822_02 euxassay_002822_03 euxassay_002822_04 euxassay_002822_05
EMAGE:31773 EMAGE:31773 EMAGE:31773 EMAGE:31773 EMAGE:31773
euxassay_002822_06 euxassay_002822_07 euxassay_002822_08 euxassay_002822_09 euxassay_002822_10
EMAGE:31773 EMAGE:31773 EMAGE:31773 EMAGE:31773 EMAGE:31773
euxassay_002822_11 euxassay_002822_12 euxassay_002822_13 euxassay_002822_14 euxassay_002822_15
EMAGE:31773 EMAGE:31773 EMAGE:31773 EMAGE:31773 EMAGE:31773
euxassay_002822_16 euxassay_002822_17 euxassay_002822_18 euxassay_002822_19 euxassay_002822_20
EMAGE:31773 EMAGE:31773 EMAGE:31773
euxassay_002822_21 euxassay_002822_22 euxassay_002822_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
metencephalon basal plate
moderate moderate
regionalmoderate expression: see section 08 18
ventral grey horn
moderate moderate
regionalmoderate expression: see section 12 13 14 17 18 weak expression: see section 15 16
kidney calyx
strong strong
regionalstrong expression: see section 09 11 12 18 19 20 21 22 moderate expression: see section 08
rectum
weak weak
regionalweak expression: see section 15
urethra of male
weak weak
regionalweak expression: see section 14 15
4th ventricle lateral recess
moderate moderate
regionalmoderate expression: see section 04 05 06 07 17 18 19 20 21 22 weak expression: see section 08 09 10 11 12 13 14 15 16
axial musculature
weak weak
regionalweak expression: see section 05 06 07 08 09 21 22 23
thalamus mantle layer
strong strong
regionalstrong expression: see section 08 09 10 16 17 18 moderate expression: see section 07 11 15 19
bladder
strong strong
regionalstrong expression: see section 14 15 16
dorsal root ganglion
weak weak
regionalweak expression: see section 09 10 11 12 13 17 18 19
submandibular gland primordium
weak weak
regionalweak expression: see section 07 08 09 10 18 19 20 21
medulla oblongata basal plate
moderate moderate
regionalmoderate expression: see section 09 10 17 18
thymus primordium
strong strong
homogeneousstrong expression: see section 12 13 14 15 16 17
trigeminal v ganglion
weak weak
regionalweak expression: see section 04 05 06 07 08 09 18 19 20 21 22 23
kidney pelvis
strong strong
regionalstrong expression: see section 10
vibrissa
moderate moderate
regionalmoderate expression: see section 05 06 07 20 21 22 weak expression: see section 04 23
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 09 10 11 12 13 15 16 17 18 19 moderate expression: see section 08
epidermis
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
lateral ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 06 07 17 18 19 20 weak expression: see section 08 09 10 11 13 14 16
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T365
Entity Detected:Cds1, CDP-diacylglycerol synthase 1 ( MGI:1921846)
Sequence:sense strand is shown

>T365
TATCTACAGAACCCTGAAGATTCATCTCACCGAGAAAGGGATCCTGCAGCCCACCTTGAAGGTGTAGCTG
CCCTTGAGGACGACGGGCTGCTGAGGAGGAGCTCACAGACCAGCTCTGACTGGAGCCGTGCACTGAGCCA
GCCTGGGGCTAAAACTCAGTAGATGCAGGCTTTGCAGATACAAAGGTTTCTTTTTCTGAATTTACAATTA
CTGTGAATATTTAACAAACACGTGGTCTAAGGTGAGTGCAGCAGTCGCCCACCCGTGAAAGATCCTGTAC
TTTTCTAAAATGTATTTTCCAATGTTAAGTTCTCTGCCCTCACTGGCCAGGTTCCATGATTTAGGTAGAC
TTGCGTTTTAAAGAGTCAAACACTATCAACTGAGGCCCTCGTAGGGTGTTGACCTTTGCCCTGGTGCTGC
CCTGGCGTAGCCATAGCTGAATGCACTGGAGGCACCTTGGGAATCGAGGTCTC
Notes:The probe template was PCR amplified from IMAGE:3155865 using vector specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3155865 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002822 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS