EMAGE mouse gene expression spatial database

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EMAGE:31782

Herpud1 ( MGI:1927406)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005097_01	euxassay_005097_02	euxassay_005097_03	euxassay_005097_04	euxassay_005097_05

euxassay_005097_06	euxassay_005097_07	euxassay_005097_08	euxassay_005097_09	euxassay_005097_10

euxassay_005097_11	euxassay_005097_12	euxassay_005097_13	euxassay_005097_14	euxassay_005097_15

euxassay_005097_16	euxassay_005097_17	euxassay_005097_18	euxassay_005097_19	euxassay_005097_20

euxassay_005097_21	euxassay_005097_22	euxassay_005097_23	euxassay_005097_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 11 12 13 14 15 16 17 moderate expression: see section 10 18

maxilla

strong

regional

strong expression: see section 05 06 07 08 09 17 18 19 20 21 22

mandible

strong

regional

strong expression: see section 03 04 05 06 07 08 09 16 17 18 19 20 21

clavicle

moderate

regional

moderate expression: see section 06 07 13 14

orbito-sphenoid

strong

regional

strong expression: see section 02 03 04 05 06 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7484
Entity Detected:	Herpud1, ( MGI:1927406)
Sequence:	sense strand is shown >T7484 CAAGTGTCGTTGTGTGGTCTCAGACGGCTGCGTCGCCGCCCGTTCGGCATCCCTGAGCGCAGTCGAGCCG CCAGCGACGCAGACATGGAGCCCGAGCCACAGCCCGAGCCGGTCACGCTGCTGGTGAAGAGTCCCAATCA GCGCCACCGCGACTTGGAGCTGAGTGGCGACCGCAGTTGGAGTGTGAGTCGCCTCAAGGCCCACCTGAGC CGAGTCTACCCCGAGCGCCCGCGTCCAGAGGACCAGAGGTTAATTTATTCTGGGAAGCTGCTGTTGGATC ACCAGTGTCTCCAAGATTTGCTTCCAAAGCAGGAAAAGCGACATGTTTTGCACCTTGTGTGCAATGTGAA GAATCCCTCCAAAATGCCAGAAACCAGCACAAAGGGTGCTGAATCCACAGAGCAGCCGGACAACTCTAAT CAGACACAGCATCCTGGGGACTCCTCAAGTGATGGTTTACGGCAAAGAGAAGTTCTTCGGAACCTTTCTC CCTCCGGATGGGAGAACATCTCTAGGCCTGAGGCTGTCCAGCAGACTTTCCAAGGCCTGGGGCCTGGCTT CTCTGGCTACACAACGTATGGGTGGCTGCAGCTCTCCTGGTTCCAGCAGATCTATGCAAGGCAGTACTAC ATGCAATACTTAGCTGCCACTGCTGCATCAGGAACTTTTGTCCCGACACCAAGTGCACAAGAGATACCTG TGGTCTCTACACCTGCTCCGGCTCCTATACACAACCAGTTTCCGGCAGAAAACCAGCCGGCCAATCAGAA TGCAGCTGCTCAAGCGGTTGTCAATCCCGGAGCCAATCAGAACTTGCGGATGAATGCACAAGGTGGCCCC CTGGTGGAGGAAGATGATGAGATAAACCGAGACTGGTTGGATTGGACCTATTCCGCAGCGACGTTTTCTG TTTTCCTCAGCACCCTTTACTTCTACTCCTCGCTGAGCAGATTCCTCATGGTCATGGGTGCCACCGTGGT CATGTACCTGCACCACGTCGGGTGGTTTCCGTTCAGACAGAGGCCAGTTCAGAACTTCCCGGATGATGGT GGTCCTCGAGATGCTGCCAACCAGGACCCCAACAATAACCTCCAGGGAGGTATGGACCCAGAAATGGAAG ACCCCAACCGCCTCCCCCCAGACCGCGAAGTGCTGGACCCTGAGCACACCAGCCCCTCGTTTATGAGCAC AGCATGGCTAGTCTTCAAGACTTTCTTTGCCTCTCTTCTTCCAGAAGGCCCACCAGCCCTAGCCAACTGA TGGCCCTTGTGCTCTGTCGCTGGTGGCTTTGACAGCTCGGACTGGATCGTCTGGCTCCGGCTCCTTTTCC TCCCCTGGCGTGGACTCGACAGAGTCATTGAAAACCCACAGGATGACATGTGCTTCTGTGCCAAGCAAAA GCACAAACTAAGACATGAAGCCGTGGTACAAACTGAACAGGGCCCCTCATGTCGTTATTCTGAAGAGCTT TAATGTATACTGTATGTAGTTTCATAGGCACTGTAAGCAGAAGGCCCAGGGTCGCATGTTCTGCCTGAGC ACCTCCCCAGATGTGTGTGCATGTGTGCTGTACATGGAAGTCATAGACGTGTGTGCATGTGTGCTCTACA TGGAAGTCATAGATGCAGAAACGGTTCTGCTGGTTCGATTTGATTCCTGTTGGAATGTTCAAATTACACT AAGTGTACTACTTTATATAATCAGTGAATTGCTAGACATGTTAGCAGGACTTTTCTAGGAGAGACTTATG TATAATTGCTTTTTAAAATGCAGTGCTTTCCTTTAAACCGAGGGTGGCGACTTGGCAGAGGTAAAACCTT TGCCGAGTTTTCTGTTCAATAAAGTTTTGCTATGAATGACTGTAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4236803 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4236803 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005097 same experiment
	EMAGE:31352 same embryo
	EMAGE:30808 same embryo
	EMAGE:30612 same embryo
	EMAGE:30567 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source