EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31789

Il4ra ( MGI:105367)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_009214_01	euxassay_009214_02	euxassay_009214_03	euxassay_009214_04	euxassay_009214_05

euxassay_009214_06	euxassay_009214_07	euxassay_009214_08	euxassay_009214_09	euxassay_009214_10

euxassay_009214_11	euxassay_009214_12	euxassay_009214_13	euxassay_009214_14	euxassay_009214_15

euxassay_009214_16	euxassay_009214_17	euxassay_009214_18	euxassay_009214_19	euxassay_009214_20

euxassay_009214_21	euxassay_009214_22	euxassay_009214_23	euxassay_009214_24	euxassay_009214_25

euxassay_009214_26

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10

thymus primordium

moderate

single cell

moderate expression: see section 09 10 11 12 13

trigeminal v ganglion

weak

single cell

weak expression: see section 04 05 06 07 08 09 18 19 20 21 22 23

adrenal medulla

moderate

regional

moderate expression: see section 10 11 12 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3535
Entity Detected:	Il4ra, ( MGI:105367)
Sequence:	sense strand is shown >T3535 TGGCCTCGAGGCAGATTCGGACGAGGCTGACCCTGGACGCTCGGAGCCTGGCTGTCTCCTCTTCCACTGG CCTGGGCTCTCCTCCCTCCCAAGGGATACAGGGGCTCACTGTGCTTGGTCCCACAGCAGTGCTGACGTTC CTAAGTCCTGGGCTTTCCTAGCTGATGTTGTCCTACCTACTCAGTCCCATTTTGTCCACCGAATAGACCT GTCACTCAAGGCTCTCAGCGGTCCTGCCATAGCTGCTGGACGCTCCCAGCTGGAAGCTGGGCCTAGAAAC TCACAGATGGCCTGGCAGTGGCATGGGAGGCCCTAAAAATTAGTGGAAATTTTGAGAGAGGACAGGTATT GCCCCACAGAGGCCATTCATTGAACAGCCAGGACTGGGACTAGAGGCAGAGCCTGCTGTCCTCCGCTCAG TTGTAGAAAGCAACAAGGACACAAACTTGATTGCCCAAAGTCACTGCCAGTTACCCACATATGACCAGAA GCCAGGGCTCCTGGGATGTGGAAGATAAACAAACACAGTTGCCGGGTGGC
Notes:	The probe template was PCR amplified from IMAGE:3166596 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3166596 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009214 same experiment
	EMAGE:31825 same embryo
	EMAGE:30054 same embryo
	EMAGE:31792 same embryo
	EMAGE:30056 same embryo
	EMAGE:29713 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source