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EMAGE:318

Pax6 paired box gene 6 ( MGI:97490)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:318

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:318EMAGE:318Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
318_voxel_strong_3D_1.wlz
318_voxel_weak_3D_1.wlz
318_voxel_possible_3D_1.wlz
318_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:318_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:29788
Entity Detected:Pax6, paired box gene 6 ( MGI:97490)
Sequence:sense strand is shown

>MGI:29788
ATGGTTTTCTAATCGAAGGGCCAAATGGAGAAGAGAAGAGAAACTGAGGAACCAGAGAAGACAGGCCAGC
AACACTCCTAGTCACATACCTACCAGCAGCAGCTTCAGTACCAGTGTCTACCAGCCAATCCCACAGCCCA
CCACACCTGTCTCCTCCTTCACATCAGGTTCCATGTTGGGCCGAACAGACACCGCCCTCACCAACACGTA
CAGTGCTTTGCCACCCATGCCCAGCTTCACCATGGCAAACAACCTGCCTATGCAACCCCCAGTCCCCAGT
CAGACCTCCTCATACTCGTGCATGCTGCCCACCAGCCCGTCAGTGAATGGGCGGAGTTATGATACCTACA
CCCCTCCGCACATGCAAACACACATGAACAGTCAGCCCATGGGCACCTCGGGGACCACTTCAACAGGACT
CATTTCACCTGGAGTGTCAGTTCCCGTCCAAGTTCCCGGGAGTGAACCTGACATGTCTCAGTACTGGCCT
CGATAACGGTAAAGAGAGAAGGAGAGAGCATGTGATCGAGAGAGGAAATTGTGTTCACTCTGCCAATGAC
TATGTGGACACAGCAGTTGGGTATTCAGGAAAGAAAGAGAAATGGCGGTTAGAAGCACTTCACTTTGTAA
CTGTCCTGAACTGGAGCCCCGGAATGGACTAGAACCAAGGACCTTTGCGTACAGAAGGCACGGTATCAGT
TGGAACAAATCTTCATTTTGGTATCCAAACTTTTATTCATTTTGGTGTATTATTTGTAAATGGGCATTGG
TATGTTATTATGAAGAAAAGAACAACACAGGCTGTTGGATCGCGGATCTGTGTTGCTCATGTGGTTGTTT
AAAGGAAACCATGATCGACAAGATTTGCCATGGATTTAAGAGTTTTATCAAGATATATTAAATACTTCTC
CCCATCTGTTTATAGTTTATGGACTGATGTTCCAAGTTTGTATTATTCCTTTGGAAATAATTGAACCTGG
GACAACA
nt 638 - nt 1624 of M77842.1
Notes:The probe used in this study was originally described by Grindley et al., 1995 [PMID:7789273] as being transcribed from a "1 kb template", "made by PCR, from a 1.6 kb cDNA clone, pm1 (Ton et al., 1991 [PMID:1612585] ), using oligonucleotide C128 (ATGGTTTTCTAATCGAAGGG) from the 3' end of the Pax-6 homeobox, and a T7 promoter oligonucleotide (AATACGACTCACTATAG) matching vector sequence". The template clone was a gift from R. Hill (Edinburgh).
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Staining procedure:autoradiography
General Information
Authors:Yasuhide Furuta, Brigid Hogan
Principal investigator:Brigid Hogan, Duke University Medical Center, 388 Nanaline Duke Bldg. Box 3709, Durham, USA NC 27710
Submitted by:Yasuhide Furuta, Department of Biochemistry and Molecular Biology, Program in Genes and Development, University of Texas MD Anderson Cancer Center, Houston, USA TX 77030
Experiment type:non-screen
References:[ PMID:7789273] Grindley JC, Davidson DR, Hill RE 1995 The role of Pax-6 in eye and nasal development. Development (121):1433-42
 [ doi:10.1016/0888-7543(92)90239-O] [ PMID:1612585] Ton CC, Miwa H, Saunders GF 1992 Small eye (Sey): cloning and characterization of the murine homolog of the human aniridia gene. Genomics (13):251-6
Acknowledgments:The template clone was a kind gift from R. Hill  Robert E Hill  MRC Human Genetics Unit Western General Hospital, Edinburgh, UK
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE