EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31800

Mybl2 myeloblastosis oncogene-like 2 ( MGI:101785)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002836_01	euxassay_002836_02	euxassay_002836_03	euxassay_002836_04	euxassay_002836_05

euxassay_002836_06	euxassay_002836_07	euxassay_002836_08	euxassay_002836_09	euxassay_002836_10

euxassay_002836_11	euxassay_002836_12	euxassay_002836_13	euxassay_002836_14	euxassay_002836_15

euxassay_002836_16	euxassay_002836_17	euxassay_002836_18	euxassay_002836_19	euxassay_002836_20

euxassay_002836_21	euxassay_002836_22	euxassay_002836_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 12 13 14 15 16

vibrissa

moderate

regional

moderate expression: see section 06 07 08 20 21 22

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23

midbrain ventricular layer

strong

regional

strong expression: see section 08 09 10 11 13 14 moderate expression: see section 07 12 15 16

hypothalamus ventricular layer

strong

regional

strong expression: see section 12 moderate expression: see section 11 13

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 08 09 10 11 14 15 16

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16 17

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 18 19 20 21

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 moderate expression: see section 11 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T421
Entity Detected:	Mybl2, myeloblastosis oncogene-like 2 ( MGI:101785)
Sequence:	sense strand is shown >T421 GGACAATGCTGTGAAGAATCACTGGAACTCCACTATCTAAGGAAAGTCGACACGGGAGGTTTCCCAGNCG AGTCCAGNGACTGCAAGCCTGTCTACTTGCTCCTGGAGCTGGAGGACAAGGAACAGCACCAGGGTGTCCA GCCGGTGGACGGCCAGGGAAGTCTCGTGAGCAGCTGGCCGCTGGTGCCCTCTATTGTGAAGGAGGAGAGC AGCGAGGAGGAGATTGCCATAGCTGCTACTTCTGCTAAAGAACTCGGACATGAGCCTGTCCCTGCTGATC TGGGAGAAGTGCGCACCCCAGAGCCCCCAGAATCTCTCAAGCGTGAATACCAGGAGTTCTCCTCCCCGGA AACGAGCCTGCCCTACNAGTGGGTGGTAGAGGCGGCAAACCTCCTCATCCCGGCTGTGGGGTCCAGCCTC TCTGAAGCTCTGGACTTGATTGAGTCGGACCCTGATGCTTGGTGCGACCTGAGTAAATTTGACCTTCCTG AAGAAC
Notes:	The probe template was PCR amplified from IMAGE:3157976 using vector specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3157976 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002836 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source