EMAGE mouse gene expression spatial database

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EMAGE:31811

Vac14 ( MGI:2157980)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_007286_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2020
Entity Detected:	Vac14, ( MGI:2157980)
Sequence:	sense strand is shown >T2020 TGGCCTCGAGGCCAGATTCGGCACGAGGTGTCACCTTAGTGACACAACCATTGGGATGATGACCAGGATT GCTGTTCTGAAGTGGCTCTACCATCTCTACATCAAAACTCCTCGAAAGATGTTCCGGCACACAGACAGCC TCTTCCCCATCTTACTTCAGACATTATCTGACGAATCTGATGAGGTTGTCTTGAAGGATCTGGAAGTGTT GGCAGAAATTGCTTCCTCCCCTGCAGGCCAGACGGATGACCCAGGTGCACCTGATGGCCCTGACCTCCGA GTCAACCACTCGGAGCTTCAGGTGCCCACCTCCGGCAGAGCCAACCTACTAAACCCTCCCAGTACCAAAG GCTTAGAAGGCTCCCCCTCCACTCCCACCATGAACTCCTACTTTTACAAGTTCATGATCAACCTTCTACA GACGTTCAGCAGTGAACGGAAGCTCCTGGAGGCCAGAGGCCCGTTCATCATCAGGCAGCTCTGCCTCCTG CTGAATGCAGAGAACATCTTCCATTCAATGGCAGACATCCTGCTTCGGGAGGAGGACCTCAAGTTCGCGT CCACC
Notes:	The probe template was PCR amplified from IMAGE:747775 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:747775 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007286 same experiment
	EMAGE:30380 same embryo
	EMAGE:30858 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source