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EMAGE:31828

Scx scleraxis ( MGI:102934)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31828 EMAGE:31828 EMAGE:31828 EMAGE:31828 EMAGE:31828
euxassay_019484_01 euxassay_019484_02 euxassay_019484_03 euxassay_019484_04 euxassay_019484_05
EMAGE:31828 EMAGE:31828 EMAGE:31828 EMAGE:31828 EMAGE:31828
euxassay_019484_06 euxassay_019484_07 euxassay_019484_08 euxassay_019484_09 euxassay_019484_10
EMAGE:31828 EMAGE:31828 EMAGE:31828 EMAGE:31828 EMAGE:31828
euxassay_019484_11 euxassay_019484_12 euxassay_019484_13 euxassay_019484_14 euxassay_019484_15
EMAGE:31828 EMAGE:31828 EMAGE:31828 EMAGE:31828 EMAGE:31828
euxassay_019484_16 euxassay_019484_17 euxassay_019484_18 euxassay_019484_19 euxassay_019484_20
EMAGE:31828 EMAGE:31828 EMAGE:31828
euxassay_019484_21 euxassay_019484_22 euxassay_019484_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
hindlimb digit 5 mesenchyme
moderate moderate
regionalmoderate expression: see section 06 07 08 22 23
extrinsic ocular muscle
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 21 23 weak expression: see section 22
sternum
moderate moderate
regionalmoderate expression: see section 12 15 16 17 weak expression: see section 11 13 14
hindlimb digit 1 mesenchyme
moderate moderate
regionalmoderate expression: see section 06 07 23
mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 16 17 18 moderate expression: see section 09 10 11 13 14 15 19 20 22 23
hindlimb digit 4 mesenchyme
moderate moderate
regionalmoderate expression: see section 06 07 08 22 23
pelvic girdle skeleton
weak weak
regionalweak expression: see section 09 10 17 18 19
rib
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 18 19 20 21 22 23
organ system
moderate moderate
regionalmoderate expression: see section 21
urethra of male
weak weak
regionalweak expression: see section 13 14 15
foot mesenchyme
strong strong
regionalstrong expression: see section 03 04 moderate expression: see section 05 21
nose
weak weak
regionalweak expression: see section 07 08 09 10 16 17 18 19 20
hindlimb digit 2 mesenchyme
moderate moderate
regionalmoderate expression: see section 06 07 23
diaphragm
moderate moderate
regionalmoderate expression: see section 17 weak expression: see section 07 08 09 10 11 12 13 14 15 16
hand mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 moderate expression: see section 04 05 21 23
hindlimb digit 3 mesenchyme
moderate moderate
regionalmoderate expression: see section 06 07 08 22 23
tail mesenchyme
strong strong
regionalstrong expression: see section 12 13 14 15 16 17
heart valve
weak weak
regionalweak expression: see section 09 10 11 12 13
lung
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55173
Entity Detected:Scx, scleraxis ( MGI:102934)
Sequence:sense strand is shown

>T55173
CCTGTGGGGACCTAAAGAGGCGGCATGAGCAGCGCACGGTGGAGCTGACGCCGCGCCCCCTGCCCGGCCC
ATGTCCTTCGCCATGCTGCGTTCAGCGCCGCCGCCGGGTCGCTACCTGTACCCTGAGGTGAGCCCGCTGT
CGGAGGATGAGGACCGCGGAAGCGAGAGCTCGGGCTCCGACGAGAAACCCTGCCGTGTGCATGCTGCGCG
CTGTGGCCTCCAGGGCGCCCGGCGGCGGGCAGGAGGACGGAGGGCCGCGGGTAGCGGGCCAGGACCCGGG
GGGCGGCCAGGCCGCGAGCCCCGGCAGCGGCACACAGCGAATGCGCGCGAGCGGGACCGCACCAACAGCG
TGAACACGGCCTTCACTGCGCTGCGCACACTCATCCCCACCGAGCCAGCGGACCGCAAGCTCTCCAAGAT
TGAGACGCTGCGCCTGGCCTCCAGCTACATTTCTCACCTGGGCAATGTGCTGCTGGTGGGTGAGGCCTGT
GGCGACGGGCAACCATGCCACTCGGGACCCGCTTTCTTCCACAGCGGTCGTGCGGGCAGCCCACTGCCAC
CGCCGCCGCCGCCACCACCACTGGCCAGAGACGGCGGCGAGAACACCCAGCCCAAACAGATCTGCACCTT
CTGCCTCAGCAACCAGAGAAAGTTGAGCAAAGACCGTGACAGAAAGACGGCGATTCGAAGTTAGAAGGAG
GAGGGTCCACAGCAGCCAAGAGGTGATGCCACTAGTGAAGGTGGATGCCACTGAAGAGTCACGGAGAGCC
CCACTTTGGACCTGATCTGAGCGCAGGTCCCCAAGAGCACGCTCACCAGGCCAGACCTGCCCCAGCTATG
AGCAGGGCCTATGAACAGAGAGATGGACAGGCGGGTGATGGCTTGACTCTAAGCAGCCGAACACTGACCC
AGGCCCACTGGGACTTTCCATGCTGGCTCTCTACCTGGGCCAAGTG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. .
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_019484 same experiment
 EMAGE:32197 same embryo
 EMAGE:31939 same embryo
 EMAGE:32217 same embryo
 EMAGE:31910 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS