EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31842

Il16 interleukin 16 ( MGI:1270855)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002804_01	euxassay_002804_02	euxassay_002804_03	euxassay_002804_04	euxassay_002804_05

euxassay_002804_06	euxassay_002804_07	euxassay_002804_08	euxassay_002804_09	euxassay_002804_10

euxassay_002804_11	euxassay_002804_12	euxassay_002804_13	euxassay_002804_14	euxassay_002804_15

euxassay_002804_16	euxassay_002804_17	euxassay_002804_18	euxassay_002804_19	euxassay_002804_20

euxassay_002804_21	euxassay_002804_22	euxassay_002804_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

trachea

strong

regional

strong expression: see section 13 14 15

hindlimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 01 02 21 22

thymus primordium

strong

regional

strong expression: see section 11 13 14 15 moderate expression: see section 12 16

vibrissa

strong

regional

strong expression: see section 03 04 05 20 21 22

pectoral girdle and thoracic body wall musculature

moderate

regional

moderate expression: see section 06 07 08 09 10 17 18 20 21 22 weak expression: see section 04 05 19

hindlimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 01 02 21

axial skeleton

strong

regional

strong expression: see section 13 14 15 moderate expression: see section 16

hindlimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 01 02 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3601
Entity Detected:	Il16, interleukin 16 ( MGI:1270855)
Sequence:	sense strand is shown >T3601 GAGACAGACAGAGGTAGAGTCTATGTCCACAACCTTCCCTAACTCCTCAGAGGTCAGGGACCCAGGTTTG CCTGAGTCTCCTCCCCCAGGTCAGCGACCCAGCACAAAAGCTTTGTCTCCAGACCCTCTCTTGAGACTGT TGACTACACAATCCGAGGATACTCAAGGACCAGGTCTCAAGATGCCAAGTCAGCGGGCACGGAGCTTCCC CCTGACCAGGACCCAGTCCTGCGAGACAAAGCTGTTGGATGAAAAGGCCAGTAAGCTTTACTCCATCAGC AGCCAGCTATCATCTGCTGTCATGAAATCCCTGCTGTGCCTTCCATCTTCAGTCTCTTGTGGCCAGATCA CCTGCATCCCCAAGGAGAGGGTGTCTCCAAAGTCACCTTGCAACAACAGCTCAGCTGCAGAGGGTTTTGG TGAAGCCATGGCCTCTGACACAGGGTTCTCTCTCAACCTTTCAGAGCTGAGAGAATATTCAGAAGGTCTC ACAGAGCCCGGGGAAACCGAGGACAGGAACCACTGTTCTTCTCAGGCC
Notes:	The probe template was PCR amplified from IMAGE:3167627 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3167627 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002804 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source