Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31847

Ptprj ( MGI:104574)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31847 EMAGE:31847 EMAGE:31847 EMAGE:31847 EMAGE:31847
euxassay_009431_01 euxassay_009431_02 euxassay_009431_03 euxassay_009431_04 euxassay_009431_05
EMAGE:31847 EMAGE:31847 EMAGE:31847 EMAGE:31847 EMAGE:31847
euxassay_009431_06 euxassay_009431_07 euxassay_009431_08 euxassay_009431_09 euxassay_009431_10
EMAGE:31847 EMAGE:31847 EMAGE:31847 EMAGE:31847 EMAGE:31847
euxassay_009431_11 euxassay_009431_12 euxassay_009431_13 euxassay_009431_14 euxassay_009431_15
EMAGE:31847 EMAGE:31847 EMAGE:31847 EMAGE:31847 EMAGE:31847
euxassay_009431_16 euxassay_009431_17 euxassay_009431_18 euxassay_009431_19 euxassay_009431_20
EMAGE:31847 EMAGE:31847 EMAGE:31847
euxassay_009431_21 euxassay_009431_22 euxassay_009431_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
kidney pelvis
moderate moderate
regionalmoderate expression: see section 08 weak expression: see section 17
kidney calyx
moderate moderate
regionalmoderate expression: see section 06 07 08 09 weak expression: see section 10 16 17 18 19
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36846
Entity Detected:Ptprj, ( MGI:104574)
Sequence:sense strand is shown

>T36846
CATCGGTCAGTCACAATTCAGTAAAGGTTAAGTTCAGCGGGTTTGAAGCCAGCCACGGACCTATCAAAGC
CTATGCTGTCATCCTCACCACCGGGGAAGCTGCCCAACCTTCTGCAGATGTTTTGAAGTACACGTATGAG
GATTTCAAAAGGGGAGCCTCGGATACTTATGTCACATACCTCATAAGAATAGAAGAGAAGGGACAGTCTC
AGGGCTTGTCTGAAGTCTTGAACTATGAAATTGATGTGGGGAACCAATCCACTACCCTCGGCTACTACAA
CGGGAGGCTGGAGCCTCTGGGCTCCTACCGGGCTTGTGTTGCTGGCTTTACCAATATTACCTACAACCTT
CAGAATGACGGCCTCATCAATGGGGATGAGAGCTATGTGTCTTTCAGTCCATATTCAGAGGCCGTGTTCT
TGCCCCAGGACCCAGGTGTCATCTGCGGAGCAGTGTTTGGATGTATCTTTGGTGCCCTGGCCATCACAGC
TGTGGGAGGCTTCATCTTCTGGAGAAAGAAAAGGACAGATGCCAAGAATAATGAAGTGTCCTTTTCTCAA
ATTAAACCTAAAAAATCCAAGTTAATCCGAGTGGAGAATTTTGAGGCCTACTTTAAGAAGCAGCAAGCTG
ACTCTAACTGTGGGTTTGCAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 100764. Forward Primer - name:100764_F_cDNA_Ptprj, sequence:CATCGGTCAGTCACAATTCAGT; Reverse Primer - name:100764_N_SP6_cDNA_Ptprj, sequence:CTGCAAACCCACAGTTAGAGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_009431 same experiment
 EMAGE:29430 same embryo
 EMAGE:30564 same embryo
 EMAGE:29431 same embryo
 EMAGE:30589 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS