EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31849

Chgb chromogranin B ( MGI:88395)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007010_01	euxassay_007010_02	euxassay_007010_03	euxassay_007010_04	euxassay_007010_05

euxassay_007010_06	euxassay_007010_07	euxassay_007010_08	euxassay_007010_09	euxassay_007010_10

euxassay_007010_11	euxassay_007010_12	euxassay_007010_13	euxassay_007010_14	euxassay_007010_15

euxassay_007010_16	euxassay_007010_17	euxassay_007010_18	euxassay_007010_19	euxassay_007010_20

euxassay_007010_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

strong

regional

strong expression: see section 01 02 03 20 21

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 16 17 18

stomach

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 10 11 14 15 16

tongue

strong

spotted

strong expression: see section 11 13 14

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 17 18

trigeminal v nerve

strong

regional

strong expression: see section 08 09 15 16

thoracic ganglion

strong

regional

strong expression: see section 12 13 14

midgut

strong

spotted

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

pancreas

strong

regional

strong expression: see section 09 10 11 15 16 17

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 06 18 19

vagus x ganglion

strong

regional

strong expression: see section 06 07 17

trigeminal v ganglion

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 16 17 18 19 20

cervical ganglion

strong

regional

strong expression: see section 08 16 17

adrenal medulla

strong

spotted

strong expression: see section 07 08 09 16 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4320
Entity Detected:	Chgb, chromogranin B ( MGI:88395)
Sequence:	sense strand is shown >T4320 GCTCTGCGGAGCCCGCACCCGCCGAGCTCCTCCTACACTCAGCTGGCGAGAGGCACCATGCAGCCGGCTA TGCTCCTCGGCCTCCTGGGCGCAGCGGCTCTGGCCGCTGTCAGCTCAGCTCCAGTGGATAACAGGGATCA CAATGAAGAAATGGTGACTCGCTGCATTATCGAAGTCCTCTCAAATGCCCTATCCAAGTCCAGTGTTCCA ACGATCACCCCTGAGTGCCGGCAAGTCCTGAAGAAGAGTGGAAAAGAGGTCAAAGGTGAAGAGAAAGGTG AAAACCAGAACTCGAAGTTTGAAGTACGGTTGCTAAGAGACCCAGCCGATGCCTCAGGAACCCGCTGGGC CTCCAGTAGGGAGGATGCAGGCGCTCCAGTAGAAGACTCTCAAGGCCAGACGAAGGTAGGCAATGAGAAG TGGACAGAAGGAGGAGGACACAGCCGAGAGGGAGTGGATGATCAAGAGAGTCTCCGTCCCTCCAACCAAC AAGCGTCCAAAGAAGCAAAGATATACCATTCGGAAGAGAGGGTGGGAAAAGAGAGGGAGAAAGAAGAAGG CAAGATTTACCCAATGGGGGAGCACAGGGAAGATGCTGGTGAAGAGAAGAAACACATTGAAGATTCTGGA GAGAAGCCGAACACCTTCTCCAACAAAAGAAGCGAGGCTTCCGCTAAGAAAAAAGATGAGTCAGTGGCCA GAGCAGATGCACACTCCATGGAGCTGGAGGAGAAGACACACAGTAGGGAGCAAAGCAGCCAGGAGAGTGG AGAGGAGACAAGGAGGCAGGAGAAACCCCAGGAGCTTACCGACCAGGACCAGAGCCAGGAAGAATCCCAG GAAGGCGAGGAAGGTGAGGAAGGCGAGGAAGGCGAGGAAGGCGAGGAAGACTCAGCCTCTGAAGTAACCA AACGCAGGCCCAGACACCACCACGGGAGAAGCGGGTCCAACAAGTCCTCTTACGAAGGGCATCCTCTATC TGAGGAAAGGAGACCCTCTCCCAAGGAGTCCAAGGAGGCAGATGTGGCCACAGTCCGTTTAGGGGAAAAA AGGAGTCACCATCTAGCTCACTACAGGGCTTCAGAAGAAGAACCTGAATATGGGGAAGAATCGAGAAGCT ATCGGGGACTACAGTACCGGGGTAGAGGAAGCGAAGAGGACAGAGCCCCAAGGCCTCGGAGTGAGGAGAG TCAGGAAAGGGAGTATAAGAGAAACCACCCCGACTCTGAACTGGAAAGCACAGCAAACAGACATGGTGAA GAAACCGAGGAAGAGAGGAGCTACGAGGGGGCAAATGGACGCCAACACAGAGGCAGGGGGCGGGAGCCAG GAGCCCATTCTGCTCTTGACACCAGAGAAGAAAAAAGGCTCTTGGATGAAGGGCACTACCCTGTTCGCGA AAGCCCAATAGATACGGCAAAAAGGTACCCACAAAGCAAATGGCAAGAGCAGGAGAAAAACTACCTCAAC TATGGCGAGGAAGGGGACCAAGGGAGATGGTGGCAGCAGGAAGAGCAGCTAGGACCGGAAGAAAGCAGGG AGGAAGTAAGGTTTCCAGACAGACAATATGAGCCCTATCCCATCACTGAAAAGAGGAAGAGGTTAGGGGC GCTGTTCAACCCGTACTTTGACCCTCTCCAGTGGAAGAATAGTGACTTTGAGAAAAGAGGCAACCCAGAT GACAGTTTTCTTGAGGATGAAGGTGAAGACAGAAATGGGGTGACCTTGACTGAGAAGAATTCCTTCCCAG AGTACAACTATGACTGGTGGGAGAGAAGGCCCTTCTCAGAGGATGTGAATTGGGGATATGAGAAGAGAAG CTTTGCCAGGGCCCCTCAGCTCGACTTGAAACGGCAATATGATGGAGTGGCCGAGTTGGACCAGCTGCTT CACTACAGGAAGAAGGCAGACGAATTTCCCGATTTCTACGACTCGGAGGAGCAGATGGGGCCTCACCAGG AGGCAAACGATGAAAAGGCCAGGGCTGACCAGAGAGTTCTGACGGCGGAAGAGAAAAAGGAACTGGAGAA CTTGGCTGCGATGGATCTGGAACTCCAGAAGATAGCGGAAAAGTTCAGCCAGCGGGGCTGACAGTTGGAG AGACGAGCCTTTCACTGAAGGACACCATTGATTCACCCACAGGCAGAAAGCAGAATTTACTGTTTATTAA ATGTTTGATGCAGTTGGAAATATCATTTATCTTTGCCAGAATGCTAATGCCATGTGACTAGCGTGACTTG TAGCGTATTCTTCCTGCAAAATAGATGTTTCACGTGTCTGTGACAATGACCGTGTTACTGTTGTTGAAAC ACAAACAATAAAAAGATTTACCTGAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4511248 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4511248 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007010 same experiment
	EMAGE:30160 same embryo
	EMAGE:31750 same embryo
	EMAGE:29579 same embryo
	EMAGE:31534 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source