EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31858

Fzd4 frizzled homolog 4 (Drosophila) ( MGI:108520)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005076_01	euxassay_005076_02	euxassay_005076_03	euxassay_005076_04	euxassay_005076_05

euxassay_005076_06	euxassay_005076_07	euxassay_005076_08	euxassay_005076_09	euxassay_005076_10

euxassay_005076_11	euxassay_005076_12	euxassay_005076_13	euxassay_005076_14	euxassay_005076_15

euxassay_005076_16	euxassay_005076_17	euxassay_005076_18	euxassay_005076_19	euxassay_005076_20

euxassay_005076_21	euxassay_005076_22	euxassay_005076_23	euxassay_005076_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

hand mesenchyme

moderate

regional

moderate expression: see section 05 weak expression: see section 01 02 03 04

vertebral axis musculature

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 19 20 21 22 23 24

head mesenchyme

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 20 21 22 23 24

kidney calyx

moderate

regional

moderate expression: see section 05 06 07 weak expression: see section 03 04 08 13 14 15 16 17 18

axial skeleton tail region

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19 20

foot mesenchyme

moderate

regional

moderate expression: see section 05 21 22 23 24 weak expression: see section 01 02 03 04

axial musculature

moderate

regional

moderate expression: see section 18

tongue mesenchyme

moderate

spotted

moderate expression: see section 11 12 13 14 15 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7514
Entity Detected:	Fzd4, frizzled homolog 4 (Drosophila) ( MGI:108520)
Sequence:	sense strand is shown >T7514 AGCGCTGGGGCGGTGAGAACAGCGCGGCGTAGAGTGCAGGCGGGCTTCGCCGAAAAGCCGGACTCGGCCG GCGCCGAGTTCTGGGATCGCCGCCTGCAGCCATGACCCTAGCAGTCCATCCCTCGGCCCGGGCTCCGGAC GTCTGATATCCCGCACATTCTCGTACAACTGCTGGAGAGGCGACTGCTGCCCCCTTGTCGCCCTTGGCGC CTTACCGCATTCCCTATCCGGAGTTGGGAGCAGCGCGGCCACCGGCGCCCCTGTGCAAACTGGGGGTGTC TGCTAGATCAGCCTCTGCCGCTGCTGCCCGCAGCTCTGGCCATGGCCTGGCCGGGCACAGGGCCGAGCAG CCGGGGGGCGCCTGGAGGCGTCGGGCTCAGGCTGGGGCTGCTGCTGCAGTTGCTCCTGCTCCTGCGGCCG ACATTGGGGTTCGGGGACGAGGAGGAGCGGCGCTGCGACCCCATCCGCATCGCCATGTGCCAGAACCTCG GCTACAACGTGACCAAGATGCCCAACTTAGTGGGACACGAGCTGCAGACAGACGCCGAGCTGCAGCTGAC AACTTTCACGCCGCTCATCCAGTACGGCTGCTCCAGCCAGCTGCAGTTCTTCCTTTGTTCGGTTTATGTG CCAATGTGCACAGAGAAGATCAACATCCCCATCGGCCCGTGCGGTGGCATGTGCCTTTCAGTCAAGAGAC GCTGTGAACCAGTCCTGAGAGAATTTGGGTTTGCCTGGCCCGACACCCTGAACTGCAGCAAGTTCCCGCC CCAGAACGACCACAACCACATGTGCATGGAAGGACCAGGTGATGAAGAGGTTCCCTTGCCCCACAAGACT CCCATCCAGCCCGGGGAAGAGTGCCACTCCGTGGGAAGCAATTCTGATCAGTACATCTGGGTGAAGAGGA GCCTGAACTGTGTTCTCAAGTGTGGCTACGATGCTGGCTTGTACAGCCGCTCAGCTAAGGAGTTCACGGA TATTTGGATGGCTGTGTGGGCCAGCCTCTGCTTCATCTCCACCACCTTCACCGTGCTGACCTTCCTGATT GATTCATCCAGGTTTTCTTACCCTGAGCGCCCCATCATATTTCTCAGTATGTGCTATAATATTTATAGCA TTGCTTATATTGTTCGGCTGACTGTAGGCCGGGAAAGGATATCCTGTGATTTTGAAGAGGCGGCAGAGCC CGTTCTCATCCAAGAAGGACTTAAGAACACAGGATGTGCAATAATTTTCTTGCTGATGTACTTTTTTGGA ATGGCCAGCTCCATTTGGTGGGTTATTCTGACACTCACTTGGTTTTTGGCAGCCGGACTCAAGTGGGGTC ATGAAGCCATTGAAATGCACAGTTCTTATTTCCACATCGCAGCCTGGGCTATTCCCGCAGTGAAAACCAT TGTCATCTTGATTATGAGACTAGTGGATGCCGATGAACTGACTGGCTTGTGCTATGTTGGGAACCAAAAC CTAGATGCCCTCACTGGCTTTGTGGTGGCTCCTCTCTTTACGTATTTGGTGATTGGAACGCTGTTCATTG CGGCGGGTTTGGTGGCCTTATTCAAAATTCGGTCCAATCTTCAAAAAGACGGGACAAAGACAGACAAGTT GGAAAGGCTAATGGTCAAGATCGGGGTCTTCTCAGTACTGTACACGGTTCCTGCAACCTGTGTGATTGCC TGTTATTTCTATGAAATCTCAAACTGGGCACTCTTTCGATATTCTGCAGATGACTCAAACATGGCAGTTG AAATGTTGAAAATTTTTATGTCTTTGCTCGTGGGCATCACTTCAGGCATGTGGATTTGGTCTGCCAAAAC TCTTCACACGTGGCAAAAGTGTTCTAACCGATTGGTGAATTCTGGGAAGGTAAAGAGAGAGAAGAGGGGG AATGGTTGGGTGAAGCCAGGAAAAGGCAACGAGACTGTGGTATAAGACTAGCCGGCTTCCTCGTTCCTCA TTGTGAAGGAAGTGATGCAGGGAATCTCAGTTTGAACAAACTTAGAAACACTTCAGCCCACACACACCCA CGTCAGCCCACCACCACTCACCCAACTCAGCATCAGAAGACCAATGGCTTCACTGCAGACTTTGGAATGG TCCAAAATGGAAAAGCCAGTTAGAGGTTTTCAAAGCTGTGAAAAATCAAAATGTTGATCACTTTAGCAGG TCACAGCTTGGAGTCCGTGGAGGTCCCGCCTAGATTCCTGAAGCCCAGGGTGATAGTGTTTGCTCCTACT GGGTGGGATTTCAACTGTGAGTTGATAACATGCAAGGAGAAAGATTAATTTTTAAAACCCTTTTAAATTT TAAATAGTAACTAGGTCTTGCAGATAGCAAAGTGATCTATAAACACTGGAAATGCTGGGTTGGGAGACGT GTTGCAGAGTTTTATAGTTTGGCTGGTCTAACATAAACATCTTCTGGCCTACACTGTCTGCTGTTTAGAA CTCTGTAGCGCACTCCCAAGAGGTGGTGTCAAAATCCTTCAGTGCCTTTGTCGTAAAACAGAATTGTTTG AGCAAACAAAAGTACTGTACTAACACACGTAAGGTATCCAGTGGATTTCTCTCTCCTGAAATTTCAACAT CCCTAATTCTAGGCAGCCCCTGTTTTCTTCACTTTAAACTAATGACTCAAAAAAAAAAAGGTTATTTTTA TAGGATTTTTTTTTGCACTGCAGCATGCCTAATGAGAGGAAAAGGGAAGGTGATTCACTTTCTGACAATC ACTTAATTCAGAGAAAAATGAGATTTGCTAAGTTGACTTACCTTACCGACCCTAGAGACCTATTGCATTA AGCAATGTTAAGCAATTGGGACTTAAAATATTTTAGTTTGTGTGATTGCATCTAGGCAGACGCCAGTCTG GAAGAACTGAAATGTTAAATTTCTTGGCAACTTTGCATTCACACAGATTAACTGTGTAATTTGTGTGTGT CAATTACAATTAAAAGCACATTCTTGGACCATGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4238940 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4238940 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005076 same experiment
	EMAGE:29252 same embryo
	EMAGE:29476 same embryo
	EMAGE:29322 same embryo
	EMAGE:29575 same embryo
	EMAGE:30895 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source