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EMAGE:31859

Gcsh ( MGI:1915383)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
olfactory cortex ventricular layer
moderate moderate
homogeneousmoderate expression: see section 10 11 15 16
kidney calyx
moderate moderate
regionalmoderate expression: see section 06 07 08 09 15 16 17 18
pons ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10
axial musculature
moderate moderate
regionalmoderate expression: see section 01 02 03 13 14 15 16 17
rest of cerebellum ventricular layer
moderate moderate
regionalmoderate expression: see section 02 03 05 06 13 14 15 weak expression: see section 04 16 17
pons mantle layer
moderate moderate
regionalmoderate expression: see section 06 13 14
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 weak expression: see section 11 12
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 05 13 14 15 weak expression: see section 06 12
thymus primordium
moderate moderate
regionalmoderate expression: see section 06 weak expression: see section 07 08 09 10
telencephalon ventricular layer
moderate moderate
homogeneousmoderate expression: see section 02 03 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
midbrain ventricular layer
weak weak
regionalweak expression: see section 06 07 08 09 10 11 12
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 09 10 13 14 15 16 17
hypothalamus ventricular layer
weak weak
regionalweak expression: see section 10 11 12
diencephalon lateral wall ventricular layer
weak weak
regionalweak expression: see section 10 11 12
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7517
Entity Detected:Gcsh, ( MGI:1915383)
Sequence:sense strand is shown

>T7517
GCGCAGTTACCCTCCGGACCTCTGACCATGTCGCTGCAAGTGTCGAGGAGCTTGCGGGTTGTGGCCTACA
GCCTGCGCACCGCCCTGGCCTTCTGCTCGCCGCGGCCCTGTGTACCGAGCGCCGCCGCCGTCCGCTCACT
GCGCACCGGATCTGCTTTGCTGTCGGTGCGTAAATTCACAGAGAAACATGAGTGGATAACGACAGAGGAA
GGTATTGGAACGGTGGGAATCAGCAATTTTGCACAGGAAGCGTTGGGAGATGTTGTTTACTGTAGTCTGC
CTGAAGTTGGGACAAAATTGAAAAAACAAGAGGAGTTTGGTGCTTTGGAGAGTGTGAAGGCTGCCAGTGA
ACTCTACTCTCCTCTGTCAGGAGAGGTAACCGAAGTCAATGAAGCTCTTGCCGAAAACCCAGGGCTTGTC
AACAAATCCTGTTACGAAGATGGTTGGCTGATCAAGATGACACTGAGTGACCCTTCAGAGCTGGATGAAT
TGATGAGTGAAGAAGCATATGAGAAATATGTGAAGTCCATTGAGGAGTGACCATGGCACCCCTCTACTCC
CAGATCAAACTGGATGTGACCTGCTCCAGCATGCTTGTCTTAAACTAGTGGAGAATAGAGGACTTGAAAT
AACTTTTAGCAATACTGATGGAAAGAAACTGTTCTTGACAGTGCTACTTAAGACCCACCCCCAACTTCCT
AAAGGATGCAGATAAACACAGTGTACAGGTTTTTGATAATTGCCATACTAAGTATTTAGACTAGGGGTTA
AGCCCTGGTGTTTAGAATTCTTAGGATCGTCTGCTGTGAAACGTGGTTATGGGGCTGGTTTTGGTGATGG
ACCACCTTCTAATCCCAGCACTCAACAGGCAGATGTTAGTTCAAGGTCGGTCTACACAACAAGTTCTAGA
CCAGCCACAGCTACATGGCGAGACCCTACCTACTGGGTATAAGGCTTTCATAATTCAAGATAATAGTGGT
GTATCTTAGATAATTTTGTTACTTGCCCATATCCACCACTGGATTTGGGTCAGAGACTTAGTGTCTTCTC
ATTGGGAATGCAAGGAAGTGTTGTACAGTGTCAGAGGCAGGAGAGTACTGTCTTTGTTTCACCATGTGCT
GTGTTAGCTGTTGTTTTCCCACCGTGAAGCACCGGCTTTGCAACACTGAGAAACAAATAAAACATTCATC
CTCCTTGTTAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4239181 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4239181 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_005077 same experiment
 EMAGE:31505 same embryo
 EMAGE:29948 same embryo
 EMAGE:30163 same embryo
 EMAGE:31555 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS