EMAGE mouse gene expression spatial database

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EMAGE:31863

Mpa2l ( MGI:4359647)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001963_01	euxassay_001963_02	euxassay_001963_03	euxassay_001963_04	euxassay_001963_05

euxassay_001963_06	euxassay_001963_07	euxassay_001963_08	euxassay_001963_09	euxassay_001963_10

euxassay_001963_11	euxassay_001963_12	euxassay_001963_13	euxassay_001963_14	euxassay_001963_15

euxassay_001963_16	euxassay_001963_17	euxassay_001963_18	euxassay_001963_19	euxassay_001963_20

euxassay_001963_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

moderate

regional

moderate expression: see section 12 13 14 15 16

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

dorsal root ganglion

weak

regional

weak expression: see section 03 04 05 06 11 12 13

lower jaw molar

moderate

regional

moderate expression: see section 07 08 17 18 19

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 14 15 16 17 18 21

upper jaw molar

moderate

regional

moderate expression: see section 07 08 09 17 18 19

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

thymus primordium

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13

renal cortex

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 14 15 16 17 18

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 12 13 14 16 17

vibrissa

moderate

regional

moderate expression: see section 07 08 09 19 20 21

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 15 16 17 18 19 20

hindgut

moderate

regional

moderate expression: see section 11 12 13 14 15 16

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 12 14 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17

lung

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4558
Entity Detected:	Mpa2l, ( MGI:4359647)
Sequence:	sense strand is shown >T4558 GGGAGCCTGAGGAGGCAGCAGCAGCTGAGAACTGCACTTGGACCTGTGCTGTGGGACCAGGTTGCTATGA CCCAACCACAAATGGCTCCCATTTGCCTTGTGGAAAACCACAATGAACAGCTGTCAGTGAACCAGGAAGC CATAGAGATTCTGGACAAGATTTCTCAGCCAGTGGTAGTCGTGGCTATTGTTGGATTGTACCGTACAGGG AAGTCCTATTTGATGAACTGTTTGGCGGGACAGAATCACGGCTTTCCTCTGGGCTCCACCGTGCAGTCTC AGACCAAGGGCATCTGGATGTGGTGCATGCCACACCCCACCAAGCCAGAGCACACCCTGGTCCTCCTGGA CACTGAGGGCCTGGGGGATGTGGAAAAGGGTGATCCTAAGAACGACTTGTGGATCTTTGCCCTCAGCGTG CTTCTGAGCAGCACCTTCATCTACAACAGCATGAACACCATCAACCACCAAGCCCTGGAGCAGCTGCATT ATGTCACAGAACTCACAGAGCTGATCAGAGCAAAGTCTTCCCCAAATCTTGCTGGAATAAAGAATTCCAC AGAGTTTGTGAGTTTCTTTCCAGACTTTGTCTGGATTGTTCGGGATTTCATGCTGGAGCTGAAGTTAAAT GGGGAAGACATCACAAGTGATGACTACCTGGAGAATGCCTTGAAGCTGATCCCAGGTGACAAACCCAGAA TGCAAGCATCCAATTCATGCAGGGAATGCATCAGACTTTTCTTTCCTAACCGGAAGTGTTTTGTCTTTGA CCGGCCAACGCATGACAAAGAACTTTTACAAAAACTTGATTCTATCACAGAAGACCAACTGGATCCTAAA TTCCAGGAAGTAACAAAGGCTTTTGTTTCTTACATCTTCACTTATGCCAAGATCAAGACCCTAAAAGAGG GAATTAAGGTCACTGGGAATAAACTAGGGATTCTGGTGACAACCTATGTGGATGCCATCAACAGTGGAGC AGTGCCTTGTCTGGATGATGCTGTGACAACTCTGGCCCAGCGTGAGAACTCAGTAGCTGTGCAGAAGGCA GCCAGCCACTACAGTGAGCAGATGGCCCAGCGACTGAGCCTTCCTACAGAAACGCTCCAGGAGCTGCTGG ATGTGCATGTAGCCTGCGAGAAGGAAGCCATTGCTGTCTTCATGGAGCATTCCTTCAAGGATGAAAATCA GCAATTCCTGAAGAAGCTGGTGGAATTAATAGGAGAGAACAAAGAGCTTTTCCTGTCGAAGAATGAAGAG GCATCAAATAAATACTGTCAAGAAGAACTGGATCGACTTTCAAAGGATTTTATGGAAAATATTTCAACAT TTTTTGTTCCTTGTGGACACAAGCTTTACATGGACAAGAGGGAGAAGATTGAACATGACTACTGGCAGGT TCCCAGGAAAGGGGTGAAGGCAAGTGAAGTCTTCCAGAGCTTTCTGCAGTCACAGGCCTTCATCGAGAGT TCCATCTTGCAGGCAGACACAGCCCTCACTGCTGGGGAGAAGGCCATTGCAGAGGAGCGTGCCCAGAAGG TGGCGGCAGAGAAGGAGCAGGATCTGCTAAGACAGAAGCAGAAGGAGCAGCAGGAGTATATGGAGGCTCA AGAGAAAAGTCACAAGGAAAACCTAGAGCAACTGAGAAGGAAGCTGGAGCAGGAGAGAGAGCAGGACATC AAAGACCATGATATGATGCTGAAGAAGCTAATGAAGGATCAAAAGGCTTTCCTTGAGGAAGGATTTAAGA AGAAAGCTGAGGAAATGAACAAAGAGATACAGCAACTGAGAGATGTCATCAAGGATAAGAAAAGAAACAC TGATCGAATTAAGGATGCTCTCTTAAATGGATTTTCTACAGTTCTTTTTCATTACCTTGTCCGTTATCTA AAGCATTTATGATTGAGTCCTTCACATTTGTGGAAATGATAGACTGTGAAATACACTTTGTTTCATACAT GCCTGCAAAGACAATGTTAGCCTTTAGAGGACACATTTTTGAAATGAGCCCTATGGAAGCTATGCAAGTA GTAATTACATGTGATTACTGTTGGCCAATTTGACTGCATAGAGAATCAGCAAGGAGACAAGGTTTTCTGT ACTCCTATGAGGACCATGTAGCCCAGGTTAGCTTGTGGGGGACTGTCTCAGGTTAATATTGTAGGAAACC CATCCTACGGGTAGGCAGCACTGTTCCTGTGGACTGAAGAAACAGAGAAAGATGAAAACAAATGACCCTA CCACTTGTGGCTTCCTGACTGTGGATGCTATACAACAAGCCAGGCTCAACTTCTCTCAGAATGCATTCTC CAGCCTTGACACAATGTATCATCATGATTCATAGACCCCAACAAACCCTTTCTTAAGTTGGTTTTGTGAG GGTATTTCATCTCAGCAAGAGGAAAAGTCATTGATATCATAATCGTGATAATCCTGGAAACTACTTAAAC ATCACTTGTTAGATTGGCAATGAAATTTAATATGACTTGATCTTGGCGTACTATATAGTCAAGAAACAAA TGTCAGTCAAGCTATCTGTAAAATGATCTGAATTGTACTAAAATGTACCCAAAAGATAAAGTCAAAACAC CATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4978977 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4978977 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001963 same experiment
	EMAGE:31862 same assay
	EMAGE:31462 same embryo
	EMAGE:31719 same embryo
	EMAGE:29892 same embryo
	EMAGE:29891 same embryo
	EMAGE:31862 same embryo
	EMAGE:31459 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source