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EMAGE:31863

Mpa2l ( MGI:4359647)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31863 EMAGE:31863 EMAGE:31863 EMAGE:31863 EMAGE:31863
euxassay_001963_01 euxassay_001963_02 euxassay_001963_03 euxassay_001963_04 euxassay_001963_05
EMAGE:31863 EMAGE:31863 EMAGE:31863 EMAGE:31863 EMAGE:31863
euxassay_001963_06 euxassay_001963_07 euxassay_001963_08 euxassay_001963_09 euxassay_001963_10
EMAGE:31863 EMAGE:31863 EMAGE:31863 EMAGE:31863 EMAGE:31863
euxassay_001963_11 euxassay_001963_12 euxassay_001963_13 euxassay_001963_14 euxassay_001963_15
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euxassay_001963_16 euxassay_001963_17 euxassay_001963_18 euxassay_001963_19 euxassay_001963_20
EMAGE:31863
euxassay_001963_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
foregut-midgut junction
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16
midgut
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18
dorsal root ganglion
weak weak
regionalweak expression: see section 03 04 05 06 11 12 13
lower jaw molar
moderate moderate
regionalmoderate expression: see section 07 08 17 18 19
submandibular gland primordium
strong strong
regionalstrong expression: see section 06 07 08 14 15 16 17 18 21
upper jaw molar
moderate moderate
regionalmoderate expression: see section 07 08 09 17 18 19
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
thymus primordium
moderate moderate
homogeneousmoderate expression: see section 08 09 10 11 12 13
renal cortex
moderate moderate
homogeneousmoderate expression: see section 05 06 07 08 09 14 15 16 17 18
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 12 13 14 16 17
vibrissa
moderate moderate
regionalmoderate expression: see section 07 08 09 19 20 21
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 15 16 17 18 19 20
hindgut
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 10 11 12 14 15 16
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 12 13 16 17
lung
strong strong
spottedstrong expression: see section 01 02 03 04 05 06 07 10 11 12 13 14 15 16 17 18 19
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4558
Entity Detected:Mpa2l, ( MGI:4359647)
Sequence:sense strand is shown

>T4558
GGGAGCCTGAGGAGGCAGCAGCAGCTGAGAACTGCACTTGGACCTGTGCTGTGGGACCAGGTTGCTATGA
CCCAACCACAAATGGCTCCCATTTGCCTTGTGGAAAACCACAATGAACAGCTGTCAGTGAACCAGGAAGC
CATAGAGATTCTGGACAAGATTTCTCAGCCAGTGGTAGTCGTGGCTATTGTTGGATTGTACCGTACAGGG
AAGTCCTATTTGATGAACTGTTTGGCGGGACAGAATCACGGCTTTCCTCTGGGCTCCACCGTGCAGTCTC
AGACCAAGGGCATCTGGATGTGGTGCATGCCACACCCCACCAAGCCAGAGCACACCCTGGTCCTCCTGGA
CACTGAGGGCCTGGGGGATGTGGAAAAGGGTGATCCTAAGAACGACTTGTGGATCTTTGCCCTCAGCGTG
CTTCTGAGCAGCACCTTCATCTACAACAGCATGAACACCATCAACCACCAAGCCCTGGAGCAGCTGCATT
ATGTCACAGAACTCACAGAGCTGATCAGAGCAAAGTCTTCCCCAAATCTTGCTGGAATAAAGAATTCCAC
AGAGTTTGTGAGTTTCTTTCCAGACTTTGTCTGGATTGTTCGGGATTTCATGCTGGAGCTGAAGTTAAAT
GGGGAAGACATCACAAGTGATGACTACCTGGAGAATGCCTTGAAGCTGATCCCAGGTGACAAACCCAGAA
TGCAAGCATCCAATTCATGCAGGGAATGCATCAGACTTTTCTTTCCTAACCGGAAGTGTTTTGTCTTTGA
CCGGCCAACGCATGACAAAGAACTTTTACAAAAACTTGATTCTATCACAGAAGACCAACTGGATCCTAAA
TTCCAGGAAGTAACAAAGGCTTTTGTTTCTTACATCTTCACTTATGCCAAGATCAAGACCCTAAAAGAGG
GAATTAAGGTCACTGGGAATAAACTAGGGATTCTGGTGACAACCTATGTGGATGCCATCAACAGTGGAGC
AGTGCCTTGTCTGGATGATGCTGTGACAACTCTGGCCCAGCGTGAGAACTCAGTAGCTGTGCAGAAGGCA
GCCAGCCACTACAGTGAGCAGATGGCCCAGCGACTGAGCCTTCCTACAGAAACGCTCCAGGAGCTGCTGG
ATGTGCATGTAGCCTGCGAGAAGGAAGCCATTGCTGTCTTCATGGAGCATTCCTTCAAGGATGAAAATCA
GCAATTCCTGAAGAAGCTGGTGGAATTAATAGGAGAGAACAAAGAGCTTTTCCTGTCGAAGAATGAAGAG
GCATCAAATAAATACTGTCAAGAAGAACTGGATCGACTTTCAAAGGATTTTATGGAAAATATTTCAACAT
TTTTTGTTCCTTGTGGACACAAGCTTTACATGGACAAGAGGGAGAAGATTGAACATGACTACTGGCAGGT
TCCCAGGAAAGGGGTGAAGGCAAGTGAAGTCTTCCAGAGCTTTCTGCAGTCACAGGCCTTCATCGAGAGT
TCCATCTTGCAGGCAGACACAGCCCTCACTGCTGGGGAGAAGGCCATTGCAGAGGAGCGTGCCCAGAAGG
TGGCGGCAGAGAAGGAGCAGGATCTGCTAAGACAGAAGCAGAAGGAGCAGCAGGAGTATATGGAGGCTCA
AGAGAAAAGTCACAAGGAAAACCTAGAGCAACTGAGAAGGAAGCTGGAGCAGGAGAGAGAGCAGGACATC
AAAGACCATGATATGATGCTGAAGAAGCTAATGAAGGATCAAAAGGCTTTCCTTGAGGAAGGATTTAAGA
AGAAAGCTGAGGAAATGAACAAAGAGATACAGCAACTGAGAGATGTCATCAAGGATAAGAAAAGAAACAC
TGATCGAATTAAGGATGCTCTCTTAAATGGATTTTCTACAGTTCTTTTTCATTACCTTGTCCGTTATCTA
AAGCATTTATGATTGAGTCCTTCACATTTGTGGAAATGATAGACTGTGAAATACACTTTGTTTCATACAT
GCCTGCAAAGACAATGTTAGCCTTTAGAGGACACATTTTTGAAATGAGCCCTATGGAAGCTATGCAAGTA
GTAATTACATGTGATTACTGTTGGCCAATTTGACTGCATAGAGAATCAGCAAGGAGACAAGGTTTTCTGT
ACTCCTATGAGGACCATGTAGCCCAGGTTAGCTTGTGGGGGACTGTCTCAGGTTAATATTGTAGGAAACC
CATCCTACGGGTAGGCAGCACTGTTCCTGTGGACTGAAGAAACAGAGAAAGATGAAAACAAATGACCCTA
CCACTTGTGGCTTCCTGACTGTGGATGCTATACAACAAGCCAGGCTCAACTTCTCTCAGAATGCATTCTC
CAGCCTTGACACAATGTATCATCATGATTCATAGACCCCAACAAACCCTTTCTTAAGTTGGTTTTGTGAG
GGTATTTCATCTCAGCAAGAGGAAAAGTCATTGATATCATAATCGTGATAATCCTGGAAACTACTTAAAC
ATCACTTGTTAGATTGGCAATGAAATTTAATATGACTTGATCTTGGCGTACTATATAGTCAAGAAACAAA
TGTCAGTCAAGCTATCTGTAAAATGATCTGAATTGTACTAAAATGTACCCAAAAGATAAAGTCAAAACAC
CATAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4978977 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4978977 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_001963 same experiment
 EMAGE:31862 same assay
 EMAGE:31462 same embryo
 EMAGE:31719 same embryo
 EMAGE:29892 same embryo
 EMAGE:29891 same embryo
 EMAGE:31862 same embryo
 EMAGE:31459 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS