EMAGE mouse gene expression spatial database

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EMAGE:31874

Cyfip1 ( MGI:1338801)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000633_01	euxassay_000633_02	euxassay_000633_03	euxassay_000633_04	euxassay_000633_05

euxassay_000633_06	euxassay_000633_07	euxassay_000633_08	euxassay_000633_09	euxassay_000633_10

euxassay_000633_11	euxassay_000633_12	euxassay_000633_13	euxassay_000633_14	euxassay_000633_15

euxassay_000633_16	euxassay_000633_17	euxassay_000633_18	euxassay_000633_19	euxassay_000633_20

euxassay_000633_21	euxassay_000633_22	euxassay_000633_23	euxassay_000633_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

lower jaw incisor

weak

regional

weak expression: see section 08 11 12 16 18 19

upper jaw incisor

weak

regional

weak expression: see section 08 12 16 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T834
Entity Detected:	Cyfip1, ( MGI:1338801)
Sequence:	sense strand is shown >T834 GTTGGCCTACTGGTTTGACCATTTGTTTATAAACTAGCAGACCAGATATTTGCATATTACAAGGTTATGG CAGGAAGTTTGCTTCTTGACAAAAGGTTACGGTCAGAATGCAAAAATCAGGGAGCCACGATCCACCTCCC CCCATCTAACCGCTATGAGACGCTGTTGAAGCAAAGGCACGTGCAGCTGCTCGGGAGATCCATTGATCTC AACCGTCTGATTACACAGCGCGTTTCAGCAGCCATGTACAAATCTCTAGAACTGGCAATTGGACGGTTTG AAAGTGAAGATTTAACATCAGTAGTCGAGCTAGATGGACTCTTAGAAATCAACCGTATGACACACAAGCT GTTGAGCAGGTACCTCACACTGGACAGCTTTGATGCCATGTTCCGGGAAGCCAACCACAACGTGTCTGCA CCTTATGGAAGAATTACCCTCCACGTCTTCTGGGAGCTAAACTATGACTTCTTGCCCAACTACTGCTATA ATGGCTCTACCAACCGGTTTGTTCGGACAGTATTACCATTTTCTC
Notes:	The probe template was PCR amplified from IMAGE:1922594 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1922594 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000633 same experiment
	EMAGE:30251 same embryo
	EMAGE:30606 same embryo
	EMAGE:31884 same embryo
	EMAGE:30231 same embryo
	EMAGE:30235 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source