EMAGE mouse gene expression spatial database

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EMAGE:31876

Ap3m2 adaptor-related protein complex 3, mu 2 subunit ( MGI:1929214)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002812_01	euxassay_002812_02	euxassay_002812_03	euxassay_002812_04	euxassay_002812_05

euxassay_002812_06	euxassay_002812_07	euxassay_002812_08	euxassay_002812_09	euxassay_002812_10

euxassay_002812_11	euxassay_002812_12	euxassay_002812_13	euxassay_002812_14	euxassay_002812_15

euxassay_002812_16	euxassay_002812_17	euxassay_002812_18	euxassay_002812_19	euxassay_002812_20

euxassay_002812_21	euxassay_002812_22	euxassay_002812_23	euxassay_002812_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 24

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 18 19 20

cervico-thoracic ganglion

weak

regional

weak expression: see section 10 11 17 18

spinal cord

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 08 19 20

thoracic ganglion

weak

regional

weak expression: see section 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 07 09 10 11 12 13 14 17 18 19 20

facial vii ganglion

moderate

regional

moderate expression: see section 22

not examined

regional

not examined expression: see section 01 02 03 24

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 16 17 18 19 20

cervical ganglion

weak

regional

weak expression: see section 09 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2126
Entity Detected:	Ap3m2, adaptor-related protein complex 3, mu 2 subunit ( MGI:1929214)
Sequence:	sense strand is shown >T2126 CTTTNTCTTTCTTACTTGTCTAAAAGTAAAATGTCAGCCTGTTTCTTAGGTCCGTTCCCTCCGGGCTCCA TCTACTCCCTTTTGTCCTCCGTCTTCAGCCACATAAGTGATGGAGGGAGAGAAAGTTCATTCGGGGAAAA AACGAGGCCAACCCCAGCGCAGCACTGACTGAGTTCTGGAGGCGTGGCCTGGAGAGCAGCGGCCTGTGAC ACTGCATTTCATCACTTTTAACACAGTTGCCGTGGGGAAAGACCGGCCTGTGATGTTTGGTTTCAGTCAC CTAAACCCAATGGAAGATGTCTCTATTGATTTCTTTTTTTTTTAAGATAGAAAGATATGAGAGGGGAAAA AAGAAGATCGGAAGAAAAATGGGTGTTAGTCCAAGAAAGAGCAGTTGAGATTTGACTCATGGGTCTGGGT TGTACCTCGGTGATCAAGCCCTGACCTGGAGTCCTGGTTCATCCTGGGTACCACCCACAATGAAGACAGA TCTGCTCGAT
Notes:	The probe template was PCR amplified from IMAGE:864554 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:864554 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002812 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source