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EMAGE:31879

Emx2 empty spiracles homolog 2 (Drosophila) ( MGI:95388)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
saccule
strong strong
regionalstrong expression: see section 07 moderate expression: see section 09 10 11 19 22 23 weak expression: see section 20 21
hindlimb digit 5 mesenchyme
strong strong
regionalstrong expression: see section 03 04 22 23
hindlimb digit 1 mesenchyme
strong strong
regionalstrong expression: see section 03 24 moderate expression: see section 23
olfactory cortex mantle layer
moderate moderate
regionalmoderate expression: see section 13 weak expression: see section 14 19
metanephros
strong strong
regionalstrong expression: see section 09 10 11 18 19 20 21 22 moderate expression: see section 12
hindlimb digit 4 mesenchyme
strong strong
regionalstrong expression: see section 03 04 22 23
hindlimb digit 2 mesenchyme
strong strong
regionalstrong expression: see section 02 24 moderate expression: see section 23
hypothalamus mantle layer
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 17 19 weak expression: see section 16 18
vomeronasal organ
strong strong
regionalstrong expression: see section 14 17
hindlimb digit 3 mesenchyme
strong strong
regionalstrong expression: see section 02 03 23 24 moderate expression: see section 22
vibrissa
weak weak
regionalweak expression: see section 06 07 09 10 11 24
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 07 09 10 11 12 13 20 21 22 weak expression: see section 02 03 04 05 06 14 16 17 18 19 23
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 10 11 12 13 14 17 18 19 20 21 moderate expression: see section 15 16
upper lip
moderate moderate
regionalmoderate expression: see section 15 16 18 19 20
mullerian tubercle
strong strong
regionalstrong expression: see section 05 06 moderate expression: see section 07 13 14 15 17 19 20 21 22 23 weak expression: see section 09 10 11 12 16
telencephalon mantle layer
strong strong
regionalstrong expression: see section 05 06 07 20 21 22 23 moderate expression: see section 24
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 07 09 10 11 12 13 15 17 19 weak expression: see section 16 18 20
diencephalon lateral wall mantle layer
moderate moderate
regionalmoderate expression: see section 09 11 12 13 15 17 weak expression: see section 16 18 20
eyelid
moderate moderate
regionalmoderate expression: see section 03 04 weak expression: see section 02
lower lip
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17 18 19 20 22 weak expression: see section 10 11 12 23 24
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55041
Entity Detected:Emx2, empty spiracles homolog 2 (Drosophila) ( MGI:95388)
Sequence:sense strand is shown

>T55041
ACAAACGAGTCCCCCATTCTCCTCGGTCCTCGCCGCGGGCAGCGGGCGGCGGAGGCAGCGCGCCGCGGTC
GCCGGGAGCAGGGAGCCCAGCGCGCCCGCTCCTCGGCGCAGCATGTTTCAGCCGGCGCCCAAGCGCTGCT
TCACCATCGAGTCGCTGGTGGCCAAGGACAGTCCCCTGCCTGCCTCGCGCTCCGAGGATCCCATCCGTCC
CGCGGCACTCAGCTACGCCAATTCCAGTCCCATAAATCCGTTCCTCAACGGCTTCCACTCGGCCGCCGCC
GCCGCCGCCGCCGGCAGGGGCGTCTACTCCAACCCGGACTTGGTGTTCGCCGAGGCGGTCTCGCACCCGC
CCAACCCCGCCGTGCCGGTGCACCCGGTGCCGCCGCCGCACGCCCTGGCCGCCCACCCCCTGCCCTCCTT
GCATTCGCCACACCCCCTTTCGCCTCGCAGCAGCGGGACCCGTCCACCTTCTACCCCTGGCTCATCCACC
GCTACCGATATCTGGGTCATCGCTTCCAAGGGAACGACACAAGTCCCGAGAGTTTCCTTTTGCACAACGC
TCTGGCCAGAAAGCCAAAGCGGATTCGAACCGCCTTCTCGCCGTCCCAGCTTTTAAGGCTAGAGCACGCT
TTTGAGAAGAACCATTACGTGGTGGGAGCGGAAAGGAAGCAGCTGGCTCACAGTCTCAGTCTTACGGAAA
CTCAGGTAAAAGTATGGTTTCAGAACCGGAGAACGAAATTCAAAAGGCAAAAGCTAGAGGAAGAAGGCTC
AGATTCTCAACAGAAGAAAAAAGGGACACACCACATTAACCGGTGGAGAATTGCTACCAAGCAGGCGAGT
CCGGAGGAAATAGATGTGACCTCAGACGATTAAAAGTCCAAACCCAATCCTACAGAAATGGACAGCACAG
AGCAAGAAAGACAGGGAGAGGAGGGGAAGAAAAACCCTACAAAACAAAAACAAACTGCACACACATCCAC
CGAGAAAGGGAGAGAGGGAATCAGAGCAGTGACGCTCGCAGCAGACTTTGGACAGCGAGGGCACTGGACC
GGGAACCGGCGCCGGGCCGGGCACCGCTTACAGGACAGCAGAGGACGGAGGCCCGATCATCAACATGCAA
CCCTCGTCTA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. .
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_019479 same experiment
 EMAGE:11464 same embryo
 EMAGE:11467 same embryo
 EMAGE:11466 same embryo
 EMAGE:11465 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS