EMAGE mouse gene expression spatial database

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EMAGE:31882

St8sia1 ( MGI:106011)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000637_01	euxassay_000637_02	euxassay_000637_03	euxassay_000637_04	euxassay_000637_05

euxassay_000637_06	euxassay_000637_07	euxassay_000637_08	euxassay_000637_09	euxassay_000637_10

euxassay_000637_11	euxassay_000637_12	euxassay_000637_13	euxassay_000637_14	euxassay_000637_15

euxassay_000637_16	euxassay_000637_17	euxassay_000637_18	euxassay_000637_19	euxassay_000637_20

euxassay_000637_21	euxassay_000637_22	euxassay_000637_23	euxassay_000637_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 15 16 17

vagus x ganglion

moderate

homogeneous

moderate expression: see section 07 08 16

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22

superior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 05 17

inferior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 05 06 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T784
Entity Detected:	St8sia1, ( MGI:106011)
Sequence:	sense strand is shown >T784 TCCTCNAGNCTGTTGGCCTACTGGATCAGACGTCCGGGAGCCAGCCGCGCCTCTTCCAGGACCCCAGCCG AGGGCGACGGTGATGCTGCAGTAAGGGCGGGAGCCACCGGCCCCACTGCTCTTAGACTCCTTCATCGCCC GCTGCCTGCAGGGAAAGAGACTAAGGCTTGTGATTTGCCCGGAGGCCACTTCCTTTAGCAAGACATCCCA GTAAAAGTTGCATTGGCGAAGATCCTTGTAGCTGACCTCTGGGGCCGTGGCGTGGGGGTTCCCCAGCCGT GCGTTTGCGAGCTGGAAAGCCAAAGGTGTGTGTGCATGGGGGGCCGGCCACTGGAGCATCCTTCACTGCC ACTTTGTCCAGCGTTGTGCTGAGGGTACACTGACCCTGGGACATCGAGGCTGCGATGAGCCCCTGCGGGC GGGCCCTACATACGTCCAGAGGGGCCATGGCCATGCTAGCTCGGAAATTCCCGCGTACCCGGCTACCCGT AGGAGCCAGTGCCCTCTGTGTGGTGGTCCTCTGTTGGCTCTACATCTTCCCTGTCTACCGGC
Notes:	The probe template was PCR amplified from IMAGE:1908051 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1908051 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000637 same experiment
	EMAGE:31878 same embryo
	EMAGE:30185 same embryo
	EMAGE:30542 same embryo
	EMAGE:31872 same embryo
	EMAGE:31762 same embryo
	EMAGE:30541 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source