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EMAGE:31883

Lgals3bp lectin, galactoside-binding, soluble, 3 binding protein ( MGI:99554)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31883 EMAGE:31883 EMAGE:31883 EMAGE:31883 EMAGE:31883
euxassay_002816_01 euxassay_002816_02 euxassay_002816_03 euxassay_002816_04 euxassay_002816_05
EMAGE:31883 EMAGE:31883 EMAGE:31883 EMAGE:31883 EMAGE:31883
euxassay_002816_06 euxassay_002816_07 euxassay_002816_08 euxassay_002816_09 euxassay_002816_10
EMAGE:31883 EMAGE:31883 EMAGE:31883 EMAGE:31883 EMAGE:31883
euxassay_002816_11 euxassay_002816_12 euxassay_002816_13 euxassay_002816_14 euxassay_002816_15
EMAGE:31883 EMAGE:31883 EMAGE:31883 EMAGE:31883 EMAGE:31883
euxassay_002816_16 euxassay_002816_17 euxassay_002816_18 euxassay_002816_19 euxassay_002816_20
EMAGE:31883 EMAGE:31883 EMAGE:31883
euxassay_002816_21 euxassay_002816_22 euxassay_002816_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
pancreas
moderate moderate
regionalmoderate expression: see section 10 11 12 13
thymus primordium
moderate moderate
homogeneousmoderate expression: see section 13 14 15 weak expression: see section 16
hindgut
strong strong
regionalstrong expression: see section 14 15 16 17 18 19
liver lobe
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 19 20 21 22 23
lateral ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 15 16 17 18 19
stomach
strong strong
regionalstrong expression: see section 09 10 11 moderate expression: see section 07 08
rectum
weak weak
regionalweak expression: see section 14 15
4th ventricle lateral recess
strong strong
regionalstrong expression: see section 13 20 21 22 moderate expression: see section 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19
axial musculature
moderate moderate
regionalmoderate expression: see section 21 22 23 weak expression: see section 05 06 07 08
midgut
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21
not examined not examined
regionalnot examined expression: see section 18
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1044
Entity Detected:Lgals3bp, lectin, galactoside-binding, soluble, 3 binding protein ( MGI:99554)
Sequence:sense strand is shown

>T1044
TCCTCNNACCTGTTGGCCTACTGGATTTTCCTGGGACGAGGCTGAGGAAAGCCACATGGTGGAGCTTTTC
TCTGCTGAACAGTCTACAGAAGGCTTCCAGTTGCAGCCAGGAAGGAGGCTCCAGGACTGCCTTCAGCCAC
TGCCTCGCTGCCTGAGGGACAGTTGAGTCCCTCTCTTGCTCCCAGGGTTGGGCTTCTAGGCCAGGCAATG
GCTCTCCTGTGGCTCCTCTCTGTGTTCTTGCTGGTTCCAGGGACTCAAGGTACAGAAGATGGAGACATGC
GCTTGGTTAACGGGGCCTCAGCCAATGAGGGCCGCGTGGAGATCTTCTACAGAGGCCGGTGGCGGACAGT
GTGTGACAACCTCTGGAACCTTTTGGATGCCCACGTCGTCTGCCGGGCCCTGGGCTATGAGAATGCCACC
CAAGCACTGGGCAGAGCTGCCTTCGGGCCAGGAAAGGGACCGATCATGCTGGATGAGGTGGAATGTACAG
GGACCGAGTCCTCACTGGCCAGTTGCAGATCCCTGGGTTGGATGGTGAGCCGCTGTGGGCAC
Notes:The probe template was PCR amplified from IMAGE:2088205 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2088205 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002816 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS