EMAGE mouse gene expression spatial database

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EMAGE:31895

Nudt2 ( MGI:1913651)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_005082_11	euxassay_005082_12	euxassay_005082_13	euxassay_005082_14	euxassay_005082_15

euxassay_005082_16	euxassay_005082_17	euxassay_005082_18	euxassay_005082_19	euxassay_005082_20

euxassay_005082_21	euxassay_005082_22	euxassay_005082_23	euxassay_005082_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 weak expression: see section 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7487
Entity Detected:	Nudt2, ( MGI:1913651)
Sequence:	sense strand is shown >T7487 GGGCTACGGATAGATCATGGCCCTGAGGGCATGTGGCTTGATCATCTTCCGAAGACACCTTATTCCCAAG ATGGACAACAGTACAATTGAATTTCTGCTGCTGCAGGCATCAGACGGCATTCATCACTGGACTCCTCCCA AAGGCCATGTGGACCCAGGAGAGAATGACTTAGAAACAGCCCTGCGAGAGACTCGGGAGGAAACAGGCAT AGAAGCAAGCCAACTGACCATCATTGAGGGGTTCAGAAGGGAGCTCAATTACGTGGCCAGGCAGAAGCCT AAAACGGTCATTTACTGGCTGGCAGAAGTGAAAGACTACAATGTAGAGATCCGCCTCTCCCAGGAGCATC AAGCCTACCGCTGGCTGGGGCTGGAGGAGGCCTGCCAGTTGGCTCAGTTCAAGGAGATGAAGGCAACACT CCAAGAAGGACACCAGTTTCTCTGCTCCACACCGGCCTGAGCTGACCAGAACAAATGCTTCCATTGCATC CTTAAGGGTTTTTCAAGATAAACCCACCCTTCCATCCAGGGAAGGATGCACGGGTCATGGCTTGTTAACA ACCAAGAGCAGATAGGTTAGTGAAATTAAAATTAACTCGGCACTCTCAGGTGACAGCAAGGAAGCCCTGG ATTCAATCCCTAGCATAGAAAATAATGACTTTAAAAAGTAAAGCAAGGATTTTACTTTAAAATAATTACT TTCAAAGCAAGAATTTTAGCTAAGTTAAAAAACAAAGGAGGGGTACGGTTTTTTAAATGACAGCCCCGAA GTATGTCTTTTGTCATTTATAAATAAACTGGAAGCAACGTACATGCCCTTTCAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:4236805 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4236805 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005082 same experiment
	EMAGE:31785 same embryo
	EMAGE:29950 same embryo
	EMAGE:31502 same embryo
	EMAGE:31535 same embryo
	EMAGE:31897 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source