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EMAGE:31897

S100g S100 calcium binding protein G ( MGI:104528)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
kidney calyx
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 15 16 17 18 19
midgut
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 20
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7488
Entity Detected:S100g, S100 calcium binding protein G ( MGI:104528)
Sequence:sense strand is shown

>T7488
CTGACTCCTGCAGCGGTTGCACTGACTGGTGAGCAGGCCAGCAAAATGTGTGCTGAGAAGTCTCCTGCAG
AAATGAAGAGCATTTTTCAAAAATATGCAGCCAAGGAAGGCGATCCGGACCAGCTCTCCAAGGAGGAGCT
AAAGCTGCTGATTCAGTCAGAGTTCCCCAGCCTCCTGAAGCAGGCTTCAAGTACTCTGGACAATCTCTTT
AAGGAGCTGGATAAGAATGGCGATGGAGAAGTTAGTTACGAAGAATTCGAAGCTTTCTTCAAAAAGCTCT
CACAATGAACAAGTCAGGAAGAAGGAGTTCCAACAGCACCTACTGATTGAACGCACGCTCTCCAATCCTG
AAGCTCTAGCTGTGCGATTATGATACATACTGTTAATAAAGCAAGTTCGGAGACACGTCTCTCTTATCAA
AAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4237050 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4237050 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_005087 same experiment
 EMAGE:31895 same embryo
 EMAGE:31785 same embryo
 EMAGE:29950 same embryo
 EMAGE:31502 same embryo
 EMAGE:31535 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS