EMAGE mouse gene expression spatial database

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EMAGE:31903

Rhebl1 ( MGI:1916409)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004769_01	euxassay_004769_02	euxassay_004769_03	euxassay_004769_04	euxassay_004769_05

euxassay_004769_06	euxassay_004769_07	euxassay_004769_08	euxassay_004769_09	euxassay_004769_10

euxassay_004769_11	euxassay_004769_12	euxassay_004769_13	euxassay_004769_14	euxassay_004769_15

euxassay_004769_16	euxassay_004769_17	euxassay_004769_18	euxassay_004769_19	euxassay_004769_20

euxassay_004769_21	euxassay_004769_22	euxassay_004769_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 04 05 06 11 12 13 14

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 15 16 17 18 19 20

cervical ganglion

moderate

regional

moderate expression: see section 06 14

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 03 04 05 15 16 17 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 07 13

spinal cord

moderate

regional

moderate expression: see section 06 07 08 09 10 weak expression: see section 11 12 13

brain

moderate

regional

moderate expression: see section 05 06 07 08 09 10 weak expression: see section 01 02 03 04 11 12 13 14 15 16 17 18 19 20 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 15 16

trigeminal v nerve

moderate

regional

moderate expression: see section 15

thoracic ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6006
Entity Detected:	Rhebl1, ( MGI:1916409)
Sequence:	sense strand is shown >T6006 CGGACGCGTGGGGCCGGGCGTAGCTTCAGCGAGCTACTCTTCTTCCCAGAGTCCACCTCGGCCTGCACCT CGGCTGATCTTCGGGTTCCCTCGGCCCGCACTCCTACGAGCCCCGCGGGGCGGCGTCCTGTCATGCCGCT GGTGCGCTACAGGAAGGTGGCCATCCTAGGGTACCGCTCCGTAGGGAAGACATCTTTGGCACATCAATTT GTGGAAGGCGAGTTCCTGGAAGGCTACGATCCTACAGTGGAGAATACTTACAGCAAGACAGTGACTCTTG GCAAAGATGAGTTTCACCTACATCTGGTGGACACAGCAGGGCAGGATGAGTACAGCATTCTGCCCTACTC GCTCATCATCGGGGTCCATGGTTACGTTCTTGTATACTCGGTTAACTCTCTGCGCAGCTTTCAGATCGTT AAGAACCTGTACCAAAAGCTACACGAAGGCCACGGTAAAACCCGGCTGTCGGTGCTGCTTGTGGGGAACA AGGCAGATCTCTCTCCTGAGAGAGAGGTGCAGGCAGTTGAAGGGAAGAAGCTAGCAGAGTCCTGGGGTGC GATGTTTATGGAGTCGTCTGCTCGGGATAATCAGCTAACTCAAGATGTCTTCATCAAAGTCATCCAGGAG ATTGCCCGGGTGGAGAATTCTTATGGACGACAAGACCGCCGATGCTATCTTATGTGAACTCTTGGCATGG AATAACTGCCCTGTTTCTGCCGCAGACATTGTCTAACCCGCAGCAGTGGGGCAGACCCTCAGGATATAAC TGCTATGGTTGCCAACTATGTCCCCTGTCCTCTGGGCACACAGTATGGCACTCTTATTTTTGCACACTCA TCCCAGGTTCTGGTAGCCTGGATGTTAATGTTTACAAAGGGGCAAGATGTCTCAGGGACCCTGGCTTCTA ACTCCTTGGTTTTCTAAACCAATTTTTACAGTCTGTACCGTAGGGATATGAGTCTTGTGCATTGATTACC CAGGACCCACCCTCCTGGGTAGGTTTTGGGTGTTGGTTGGGTAAGGGTGTTTGGTTCCTTGGCAGTTCTG AAGTAGAACTGTCTGCTGGGGTAACAATGTATATTTCCAAATAAACTGAGAAATCTTTTGTTGAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3487009 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3487009 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004769 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source