Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31926

Camkk2 ( MGI:2444812)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31926 EMAGE:31926 EMAGE:31926 EMAGE:31926 EMAGE:31926
euxassay_003670_01 euxassay_003670_02 euxassay_003670_03 euxassay_003670_04 euxassay_003670_05
EMAGE:31926 EMAGE:31926 EMAGE:31926 EMAGE:31926 EMAGE:31926
euxassay_003670_06 euxassay_003670_07 euxassay_003670_08 euxassay_003670_09 euxassay_003670_10
EMAGE:31926 EMAGE:31926 EMAGE:31926 EMAGE:31926 EMAGE:31926
euxassay_003670_11 euxassay_003670_12 euxassay_003670_13 euxassay_003670_14 euxassay_003670_15
EMAGE:31926 EMAGE:31926 EMAGE:31926 EMAGE:31926 EMAGE:31926
euxassay_003670_16 euxassay_003670_17 euxassay_003670_18 euxassay_003670_19 euxassay_003670_20
EMAGE:31926 EMAGE:31926 EMAGE:31926 EMAGE:31926
euxassay_003670_21 euxassay_003670_22 euxassay_003670_23 euxassay_003670_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
neural retina
moderate moderate
regionalmoderate expression: see section 01 02 03 23 24
spinal cord mantle layer
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16
vestibulocochlear viii ganglion
weak weak
regionalweak expression: see section 17 18
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 07 18
trigeminal v nerve
weak weak
regionalweak expression: see section 09 17
testis
weak weak
regionalweak expression: see section 06 07 08 19 20 21
telencephalon
moderate moderate
regionalmoderate expression: see section 01 02 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 03 04 05 06 07
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 09 10 11 12 16 17 18 weak expression: see section 07
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 05 20 weak expression: see section 06
midbrain
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 06 07
vagus x ganglion
weak weak
regionalweak expression: see section 09 17
diencephalon
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16 17 18 weak expression: see section 07
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 18 19 20 22 weak expression: see section 06 07 09 17
hindbrain
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 weak expression: see section 04 05 06 07
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7020
Entity Detected:Camkk2, ( MGI:2444812)
Sequence:sense strand is shown

>T7020
GCTGCTGATGGGAGCGCCCCCGCCTGGCTGATGAGGTTGGTACACACACCATGTCATCATGTGTCTCTAG
CCAGCCCACCAGCGACCGGGTGGCCCCCCAGGATGAGCTGGGAAGTGGGGGTGGCAGCCGGGAAGGCCAG
AAGCCCTGTGAGGCACTGCGGGGACTCTCATCCTTAAGTATCCACTTGGGCATGGAATCCTTCATCGTGG
TCACCGAGTGTGAGCCAGGCCGGGGTGTGGACCTCAACCTGGCCAGAGACCAGCCTCCGGAGGCCGATGG
CCAGGAACTCCCCCTCGAAGCCTCGGACCCTGAGTCCCGGTCCCCGCTTTCTGGTCGCAAGATGTCCCTG
CAGGAGCCGTCCCAGGGCGGGCCGGCATCCAGCAGCAACAGCCTGGACATGAATGGACGCTGCATCTGCC
CATCCCTGTCCTACTCACCAGCCAGCTCCCCACAGTCCTCTCCCCGGATGCCCCGGCGGCCCACAGTAGA
GTCACACCACGTCTCCATTACCGGTTTGCAGGACTGCGTGCAGCTGAATCAGTACACCCTGAAGGATGAA
ATTGGAAAGGGCTCCTATGGTGTTGTCAAGCTGGCCTACAATGAAAATGACAATACTTATTACGCAATGA
AAGTGCTGTCCAAAAAGAAGCTGATCCGACAGGCTGGCTTCCCACGTCGCCCCCCACCACGAGGAGCTCG
CCCAGCCCCAGGGGGCTGCATCCAGCCCAGGGGCCCCATCGAGCAAGTGTACCAGGAGATTGCTATCCTC
AAAAAGCTGGATCATCCCAACGTGGTGAAGCTGGTAGAGGTCCTGGATGACCCTAATGAGGACCATCTGT
ACATGGTGTTTGAGTTGGTGAACCAAGGGCCTGTAATGGAAGTGCCCACCCTCAAGCCACTGTCCGAAGA
CCAGGCCCGCTTCTACTTCCAGGATCTGATCAAAGGCATAGAGTACTTGCATTACCAGAAGATCATCCAT
CGGGACATCAAACCTTCCAACCTCCTGGTGGGGGAAGACGGGCACATCAAGATTGCCGACTTCGGTGTAA
GCAACGAGTTCAAGGGCAGCGACGCCTTGCTGTCTAACACCGTGGGCACACCTGCCTTCATGGCACCCGA
GTCGCTCTCAGAGACCCGCAAGATCTTCTCCGGAAAGGCGTTGGATGTTTGGGCCATGGGTGTGACGCTG
TACTGCTTTGTCTTTGGCCAGTGCCCTTTCATGGATGAACGAATCATGTGTTTGCACAGTAAGATCAAGA
GTCAGGCCCTGGAGTTTCCCGACCAGCCCGATATAGCCGAAGACTTGAAAGATCTGATCACTCGGATGTT
GGACAAAAATCCAGAGTCCAGGATTGTGGTGCCTGAAATCAAGCTGCACCCTTGGGTTACGAGGCACGGG
GCCGAGCCATTGCCGTCGGAGGACGAGAACTGCACACTGGTCGAGGTGACCGAAGAGGAGGTCGAGAATT
CAGTCAAACACATTCCCAGCCTGGCAACTGTGATCCTGGTAAAGACCATGATTCGAAAGCGCTCATTCGG
GAACCCGTTCGAAGGTAGCCGCCGGGAGGAACGTTCCCTGTCAGCGCCTGGAAACCTGCTCACGAAGCAA
GGCAGCGAAGACAGCCCCCGGGGCCCAGAGCCGGCCCCTGTGGGGGAGGAGGAAGTGCTCTTGTGAAAGG
TGGTCCCTGCGTGGAAAGTTGGGGGGCTCCGGCCCCTGGCTCCCCACCACGCATGCCTCCACTGCAGCCC
GAGGAGGTGATGGAGCCGGAGTAGCTGCGTGGACCACCTGACCTCGCATGCCTGGCTGTGTGGCCCTGCA
CGGGGCTGCTGCACCCTGCGTTTCCATAGCAGCATGTCCTACGGAAACCAAGCACGTGTGTAGAGCCTTG
ACTGTCGTCTCTGGTTATCTGTTTTTTTTGTTTTTGTTTTTTTTGTTGTTGTTGCTGTTGTTTTTCTTGT
GGTTTTTCTTTTTCTTTTTTTTCTTTCCCTTTGTTGTCTAAGGGGACAAAGAAAAAAAAAAGGACTTGAC
TCCATGTCGTCGACTTTGGCCGCTGGCTGGCCGGACCGGCGGGTGTGAGGAGTTGCAAACCGAAACCAAC
ATGCATTTTGGGACAATTGCTTTTTAAAATGTTTTTAATGCCAAAAACAAACAAACAAACAAAAACCCGC
CCCCTCCATTGTGAATTCAGAACCATGTCAGATGTACCAGGTGAATGTGTGTGGGGTTTGAGCTGCGTGG
AGAATCACGTGGGGCGAGTCCTGCCGGCTGGGGCTACACTGGGGGGGGGGGGGCGGATTAGAAGGATCGG
TGTGACCCACGTACAAACCCCATGCCTGGGCGCCTTCCAGGATCATGGAAAGACAGGGCTAGCCCTCAGT
AGCTGCAGGGAACTTCCATGTCATTTATTTGTAATGAGGACTTTTTAAGTGTGTGTGTGTGTTTTTTTTA
ATGGGTATAATTATAGTCAGGAAAACTACAAGGGCTTGCAGTTGAAATGCTCGAGGGGTACTTTGCACCC
CTGAATGCGAGCATTCCTCAGCTGTGGTGGTAGAGGTCTGTCTGGAGGCTGAACACACTTCATGTTAGCT
GCTCCAACTGGGTTTCTGAGATTGTCAGCTAAGCTAATTCTCTTTGTAAATTGGCAAAAAAAAAAAAAAA
A
Notes:The probe template was PCR amplified from IMAGE:3993474 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3993474 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_003670 same experiment
 EMAGE:29380 same embryo
 EMAGE:31907 same embryo
 EMAGE:31927 same embryo
 EMAGE:29378 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS