EMAGE mouse gene expression spatial database

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EMAGE:31929

Arts1 ( MGI:1933403)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002116_01	euxassay_002116_02	euxassay_002116_03	euxassay_002116_04	euxassay_002116_05

euxassay_002116_06	euxassay_002116_07	euxassay_002116_08	euxassay_002116_09	euxassay_002116_10

euxassay_002116_11	euxassay_002116_12	euxassay_002116_13	euxassay_002116_14	euxassay_002116_15

euxassay_002116_16	euxassay_002116_17	euxassay_002116_18	euxassay_002116_19	euxassay_002116_20

euxassay_002116_21	euxassay_002116_22	euxassay_002116_23	euxassay_002116_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 11 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5515
Entity Detected:	Arts1, ( MGI:1933403)
Sequence:	sense strand is shown >T5515 GGCGGCCAGACTGGCGGCTGTGATGAAGGTAATTTGAGCAAGAAGATAGTGTCTCTGCTCCTCAAATAGC CACTTGCAAGCATGCCCTCTCTTCTTCCCCTAGTATTGACATTTTTATCTGTGTCATCTCCTTCTTGGTG TCAGAACAGTGATATAGAATCTCTAAAAGCTAGTAATGGAGACTCATTCCCTTGGAATAATATGCGACTT CCTGAGTATATGACCCCGATTCATTATGATCTCATGATCCATGCAAACCTCAGCACTCTGACTTTCTGGG GAAAAACAGAAGTAGAAATCATAGCTAGCCGGCCCACCAGCACCATTATTATGCATAGTCACCACCTGCA AATATCTAAGGCCACCCTCAGAAGAGGAGCTGGAGAGATGCTGTCTGAAGAACCACTGAAGGTCCTGGAA TATCCTGCTCATGAGCAAGTTGCACTGCTGGCTGCCCAGCCGCTTCTTGCTGGGTCCCTGTACACAGTTA TCATCGACTATGCTGCCAACCTGTCTGAGAGTTTCCATGGATTCTATAAGAGCACCTACAGAACACAGGA AGGTGAAATGAGAATACTTGCAGCAACACAGTTTGAACCCACAGCTGCTAGAATGGCTTTTCCCTGCTTT GATGAACCTGCCCTCAAGGCAAGTTTTTCCATCAAGATAAAGAGGGATCCAAGGCACCTGGCCATCTCCA ACATGCCCCTGGTGAAATCTGTGAATGTTGCTGAAGGACTCATAGAAGACCATTTTGACATCACTGTGAA GATGAGTACCTACCTAGTGGCCTTCATCATTTCTGATTTTAAGTCTGTGAGCAAGATGACTAAGAGTGGA GTCAAGGTTTCTGTGTATGCTGTGCCAGACAAGATAAATCAAGCCGATTATGCTCTGGATGCTGCAGTGA CTCTTCTAGAGTTTTATGAGGATTATTTCAACATTCCATATCCTTTACCCAAGCAAGATCTTGCTGCAAT CCCAGACTTTCAGTCTGGTGCTATGGAAAACTGGGGACTGACCACATACAGAGAGTCCTCTCTGTTATAC GATAAAGAAAAGTCTTCTGCATCTAGTAAGCTTGGCATCACAATGATTGTGTCCCATGAACTGGCTCACC AGTGGTTCGGGAACCTGGTCACCATGGAATGGTGGAATGACCTTTGGCTCAATGAAGGATTTGCCAAATT TATGGAGTTTGTGTCTGTCACTGTGACCCATCCTGAACTAAAAGTTGAAGACTATTTCTTTGGCAAGTGT TTTAATGCAATGGAAGTTGATGCATTAAACTCCTCTCACCCTGTATCCACACCTGTGGAGAATCCTGCGC AGATTCGGGAGATGTTTGATGATGTTTCTTATGAAAAGGGAGCTTGTATTCTGAATATGCTAAGAGATTA TCTGAGTGCAGATACATTTAAAAGAGGAATTGTACAGTATCTCCAAAAGTATAGTTATAAAAACACAAAA AACGAGGACCTGTGGAATAGCATGATGCATATTTGCCCTACAGATGGCACACAAACAATGGATGGCTTCT GCTCTAGAAGCCAACACTCATCATCAACCTCACACTGGCGTCAGGAGGTTGTAGATGTAAAGACCATGAT GAACACATGGACACTGCAGAAGGGCTTTCCTCTGATAACCATCACTGTGAGTGGGCGGAATGTGCACATG AAGCAAGAACACTACATGAAGGGTTCAGAACGCTTCCCAGAGACTGGGTATTTGTGGCATGTTCCACTGA CATTCATTACCAGCAAATCAGACTCAGTCCAGAGATTTTTGCTGAAGACAAAGACAGATGTGCTCATCCT CCCAGAAGCAGTGCAGTGGATCAAATTTAACGTGGGAATGAATGGCTATTACATTGTGCATTACGCGGAT GATGGATGGGCTTCTCTGAGTGGCCTTTTAAAAGAAGCGCACACAACAATCAGCAGTAATGATCGGGCAA GTCTCATCAACAATGCATTTCAGCTGGTCAGCATTGAAAAGTTATCAATAGAAAAAGCCCTGGATTTAAC CCTGTACTTGAAAAATGAAACTGAAATTATGCCCATATTCCAAGCTTTGAATGAACTCATACCTATGTAT AAGTTAATGGAGAAAAGAGATATGATTGAGGTGGAAACTCAATTTAAGGACTTCCTTCTCAAGTTGCTGA AGGACCTTATTGATAAGCAGACTTGGACAGATGAAGGCTCTGTCTCAGAGAGGATGCTCAGGAGCCAGCT CCTCCTCCTCGCATGTGTGCGCAATTATCAGCCCTGTGTGCAGAGGGCAGAGCGCTATTTCAGAGAGTGG AAGTCATCTAATGGAAACATGAGCATCCCTATTGATGTGACCTTGGCTGTGTTTGCTGTCGGGGCCCAAA ACACAGAAGGCTGGGATTTTCTTTATAGTAAATATCAGTCTTCTTTGTCTAGTACTGAGAAAAGCCAAAT TGAATTTTCCCTCTGTACAAGCAAAGATCCAGAAAAACTTCAGTGGCTTCTAGATCAAAGTTTTAAGGGA GAGATAATAAAAACTCAGGAATTTCCACATATTCTCACACTCATTGGCAGAAACCCAGTGGGTTATCCAT TGGCCTGGAAGTTTCTGAGGGAAAATTGGAATAAGCTTGTACAGAAATTTGAACTTGGCTCATCGTCCAT AGCTCACATGGTAATGGGAACAACAGACCAGTTTTCCACCAGAGCACGTCTTGAAGAGGTAAAAGGATTC TTCAGTTCCTTGAAAGAAAACGGTTCTCAGCTCCGTTGTGTTCAACAAACCATTGAGACCATTGAAGAAA ACATACGATGGATGGATAAGAATTTTGATAAAATAAGACTGTGGCTGCAAAAAGAAAAGCCAGAGTTGCT GTGACAATATGTTTCTTGCCAAGTTCCAGAATTTATGATGAGCAAAATTTTGTTCAATTTGAGTATTTTT AAACTAAAGATAGTTGTTTTGGCTACTAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6397585 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6397585 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002116 same experiment
	EMAGE:29934 same embryo
	EMAGE:32242 same embryo
	EMAGE:29774 same embryo
	EMAGE:29931 same embryo
	EMAGE:29944 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source