EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31938

Srpx2 sushi-repeat-containing protein, X-linked 2 ( MGI:1916042)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004774_01	euxassay_004774_02	euxassay_004774_03	euxassay_004774_04	euxassay_004774_05

euxassay_004774_06	euxassay_004774_07	euxassay_004774_08	euxassay_004774_09	euxassay_004774_10

euxassay_004774_11	euxassay_004774_12	euxassay_004774_13	euxassay_004774_14	euxassay_004774_15

euxassay_004774_16	euxassay_004774_17

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

mandible

strong

regional

strong expression: see section 01 02 03 04 05 06 12 13 14 15 16 moderate expression: see section 07 08 09 10 11 17

stomach

weak

single cell

weak expression: see section 02 03

tongue

moderate

regional

moderate expression: see section 07 08 09 10 weak expression: see section 11

clavicle

moderate

regional

moderate expression: see section 11 12 13

rib

strong

regional

strong expression: see section 01 02 12 13 14 moderate expression: see section 11

leg

moderate

regional

moderate expression: see section 01 02 03 04 14 15 16 17

testis

moderate

regional

moderate expression: see section 02 03 04 05 11 12 13

midgut

weak

single cell

weak expression: see section 07 08 09 10 11 12

peritoneal component

moderate

regional

moderate expression: see section 13

arm

moderate

regional

moderate expression: see section 15 16 17

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 08 09 10 11 12 13 14 15 16 17

maxilla

strong

regional

strong expression: see section 02 03 04 06 07 12 13 15 16 moderate expression: see section 05 08 10 11

tail

weak

single cell

weak expression: see section 08 09 10 11 12

orbito-sphenoid

strong

regional

strong expression: see section 01 02 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6031
Entity Detected:	Srpx2, sushi-repeat-containing protein, X-linked 2 ( MGI:1916042)
Sequence:	sense strand is shown >T6031 CCGACTGACTGATGAGAGGAGCAGAGAGCAAAGAAAAAGAAGAGAGATGAACGAGACCACAAAAATCATA AAATAAAAAGCAGATGCATGTTCCTGCTCTTTTCAAAGCTTCCAGTAGAACGATAGCTCCCTCCGATGTC ATATGCCTGCTTCCTCTCACTTTTGGGAGTTCATAGCTGGTTCTGCTCTGAAAGTCATTCCCCTTTATCC TGGTACTTCTGGCCACATAGCCCTAGTCTTGTCTTCTGAAGCTTCCCTGTCGACGATGATGACCAGTCCA CTGACTCAGAGAGGTGCTCTCTCACTGCTGCTCCTCCTAATGCCCGCAGTGACACCAACATGGTACGCAG GCTCAGGTTACTCTCCAGATGAAAGCTACAATGAAGTATATGCAGAAGAGGTCCCCGCTGCCCGTGCCCG TGCCCTGGACTACAGAGTCCCCCGATGGTGTTACACATTGAATATCCAGGATGGAGAAGCCACATGCTAC TCACCAAGGGGAGGAAATTATCACAGTAGCCTAGGCACACGTTGTGAGCTCTCCTGTGACCGGGGCTTTC GATTGATTGGACGGAAGTCAGTGCAATGTTTGCCAAGCCGGCGTTGGTCTGGAACTGCCTACTGCAGGCA GATAAGGTGCCACACACTGCCATTCATCACTAGTGGCACCTATACGTGCACCAATGGAATGCTGCTTGAC TCCCGTTGTGACTATAGCTGTTCTAGTGGCTACCACCTAGAAGGAGATCGCAGCCGCATCTGCATGGAAG ATGGGCGATGGAGTGGAGGCGAGCCTGTATGTGTAGACATAGATCCACCCAAGATCCGTTGTCCTCACTC TCGTGAGAAAATGGCAGAGCCAGAGAAACTAACTGCTCGAGTATACTGGGACCCACCCTTAGTGAAAGAT TCTGCTGATGGTACCATCACCAGGGTGACACTTCGGGGTCCAGAGCCTGGTTCTCACTTCCCTGAAGGAG AACATGTGATTCGTTATACTGCCTATGACCGAGCCTACAACCGGGCCAGCTGCAAATTCATTGTAAAAGT ACAAGTGAGACGCTGTCCCATTCTGAAGCCACCACAGCACGGCTACCTCACCTGCAGCTCAGCGGGGGAC AACTATGGTGCCATCTGTGAATACCACTGTGATGGTGGTTATGAACGCCAGGGGACCCCATCCCGAGTCT GCCAGTCAAGTCGACAGTGGTCAGGAACACCACCTGTCTGTACTCCTATGAAGATTAATGTCAATGTTAA CTCGGCTGCTGGCCTCCTGGATCAGTTCTATGAGAAACAGCGACTCCTCATAGTCTCTGCTCCTGATCCC TCCAATCGGTACTACAAAATGCAAATCTCTATGCTGCAGCAATCCACCTGTGGACTCGACCTGCGGCATG TGACCATTATTGAGCTGGTGGGACAGCCACCTCAGGAGGTGGGGCGCATCCGGGAGCAACAACTATCAGC AGGCATCATTGAGGAGCTCAGGCAATTCCAGCGCCTCACTCGTTCCTACTTCAACATGGTGTTAATTGAC AAGCAGGGTATTGACCGGGAACGCTATATGGAACCTGTCACCCCTGAGGAAATCTTCACATTCATCGATG ACTACCTGCTGAGCAATGAGGAGCTGGCACGGAGAGTGGAGCAGAGGGACCTATGCGAGTGACCTTGAGT CAGGGTGTGGCTGAAGCACCTGTCCTAGGGAGCTTAAACTAAGGAGGAAATGTCGTGTCCTCCCCCCCCC ACACACACACACACACACTGTTGGGAGCAGCCCTCGGGTGGGGGTGAGGTTTGCCAAATCTAGGACAGTT CCAGGCAGCATTTTAGGGCAGAATATTCATTTCCCTAAGCAAGTCTCCACTTCGGGTAGCACTGGAGGAG CCTATGAAACTGAGGACATGCCCTGGGTGGTGAGTTGTAGACCGAAGGCCTTCTTCACCTGCCTGGAGCC TCTCTCTCTAGACTCTTCCCAAAGCCTTTCAGATAAGTAAATACCAAATTCATTCCTTTAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:3488526 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3488526 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004774 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source