EMAGE mouse gene expression spatial database

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EMAGE:31942

Aqp4 ( MGI:107387)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004747_01	euxassay_004747_02	euxassay_004747_03	euxassay_004747_04	euxassay_004747_05

euxassay_004747_06	euxassay_004747_07	euxassay_004747_08	euxassay_004747_09	euxassay_004747_10

euxassay_004747_11	euxassay_004747_12	euxassay_004747_13	euxassay_004747_14	euxassay_004747_15

euxassay_004747_16	euxassay_004747_17	euxassay_004747_18	euxassay_004747_19	euxassay_004747_20

euxassay_004747_21	euxassay_004747_22	euxassay_004747_23	euxassay_004747_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

moderate

regional

moderate expression: see section 07 08 09 13 14 15 weak expression: see section 10 11 12

midbrain

strong

regional

strong expression: see section 11 12 13 moderate expression: see section 09 10 14

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 18 19 20 21 weak expression: see section 08 17

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 14 15 16 17 18

hindbrain

strong

regional

strong expression: see section 11 12 13 moderate expression: see section 09 10 14 15 16 17

stomach

strong

regional

strong expression: see section 04 05 06 07 08

metanephros

moderate

spotted

moderate expression: see section 06 14

lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 13 14 15 16 17 18 19 20 21 weak expression: see section 09 10 11 12

spinal cord

moderate

spotted

moderate expression: see section 08 09 10 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4582
Entity Detected:	Aqp4, ( MGI:107387)
Sequence:	sense strand is shown >T4582 TGAGTGACAGAGCTGCGGCAAGGCGGTGGGGTAAGTGTGGACATTCCTGCAGTAGAGAGAGCATCATGGT GGCTTTCAAAGGAGTCTGGACTCAGGCTTTCTGGAAGGCAGTCTCAGCAGAATTTCTGGCCACGCTTATC TTTGTTTTGCTCGGTGTGGGATCCACCATAAACTGGGGTGGCTCAGAAAACCCCTTACCTGTGGACATGG TCCTCATCTCCCTTTGCTTTGGACTCAGCATTGCTACCATGGTGCAGTGCTTTGGCCACATCAGTGGTGG CCACATCAATCCCGCTGTGACTGTAGCCATGGTGTGCACACGAAAGATCAGCATCGCTAAGTCCGTCTTC TACATCATTGCACAGTGCCTGGGGGCCATCATTGGAGCCGGCATCCTCTACCTGGTCACACCTCCCAGTG TGGTTGGAGGATTGGGAGTCACCACGGTTCATGGAAACCTCACCGCTGGCCATGGGCTCCTGGTGGAGTT AATAATCACTTTCCAGTTGGTGTTCACTATTTTTGCCAGCTGTGATTCCAAACGAACTGATGTTACTGGT TCAATAGCTTTAGCAATTGGATTTTCCGTTGCAATTGGACATTTGTTTGCAATCAATTATACTGGAGCCA GCATGAATCCAGCTCGATCTTTTGGACCCGCAGTTATCATGGGAAACTGGGCAAACCACTGGATATATTG GGTTGGACCAATCATGGGCGCTGTGCTGGCAGGTGCCCTTTATGAGTATGTCTTCTGTCCTGATGTGGAG CTCAAACGTCGCCTTAAGGAAGCCTTCAGCAAAGCCGCGCAGCAGACAAAAGGGAGCTACATGGAGGTGG AGGACAACCGGAGCCAAGTGGAGACGGAAGACTTGATCCTGAAGCCCGGAGTGGTGCATGTGATTGACAT TGACCGTGGAGAAGAGAAGAAGGGGAAAGACTCTTCGGGAGAGGTATTGTCTTCCGTATGACTAGAGGAC AGCACTGAAGGCAGAAGAGACTCCCTAGACCTGGCCTCAGATTTCCTGCCACCCATTAAGGAAACAGATT TGTTATAAATTAGACACTTGCGGGTTTCTTGCTTCACACCTTGTTACACAGTTTAAATAACACATATTTT ACTATTATCAATGGGGGGGGTGAGAAAAAGCCTATAATGGATATAAAATTTTAAAACAGAAATCTTTTTG AATGTATCCCCAAAGCAACTATGCAACTAGTGTATTGGCTTCCCTTTCATTAATAACTGAAATTATGAAC CAAGATCTGGTCAAGTTTTGCCGTGCAGAGCATATGGACACCTCTGTGAGGAAGCTGGCATTGTCCATCG TCTTGACTATTGACTTCATTGGATTGATTTAAAAATGACAAAATGCAGTATGTCACAGAATCATGTGCAT TCAGGAGAAGACATGCAGTAACTTCTTCCACGAGCTATTCCTTATTTATAAACTACCTCGGAGGGGGAAA ACATTAGCAAGGGCCATTGCTAATACATCATTTGTATTTATATATCTGACTGTCAGAACCTAACTCTAAT TCACTATAATCCTCTCCCCCTCAGAATCCAAGAACCCATAGGGCTAAAAGTAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4985265 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4985265 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004747 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source