EMAGE mouse gene expression spatial database

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EMAGE:31952

Fgf18 fibroblast growth factor 18 ( MGI:1277980)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_010192_01	euxassay_010192_02	euxassay_010192_03	euxassay_010192_04	euxassay_010192_05

euxassay_010192_06	euxassay_010192_07	euxassay_010192_08	euxassay_010192_09	euxassay_010192_10

euxassay_010192_11	euxassay_010192_12	euxassay_010192_13	euxassay_010192_14	euxassay_010192_15

euxassay_010192_16	euxassay_010192_17	euxassay_010192_18	euxassay_010192_19	euxassay_010192_20

euxassay_010192_21	euxassay_010192_22	euxassay_010192_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

tail mesenchyme

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36378
Entity Detected:	Fgf18, fibroblast growth factor 18 ( MGI:1277980)
Sequence:	sense strand is shown >T36378 CTTTCTACTGCTGTGCTTCCAGGTTCAGGTGTTGGCAGCCGAGGAGAATGTGGACTTCCGCATCCACGTG GAGAACCAGACGCGGGCTCGAGATGATGTGAGTCGGAAGCAGCTGCGCTTGTACCAGCTCTATAGCAGGA CCAGTGGGAAGCACATTCAAGTCCTGGGCCGTAGGATCAGTGCCCGTGGCGAGGACGGGGACAAGTATGC CCAGCTCCTAGTGGAGACAGATACCTTCGGGAGTCAAGTCCGGATCAAGGGCAAGGAGACAGAATTCTAC CTGTGTATGAACCGAAAAGGCAAGCTCGTGGGGAAGCCTGATGGTACTAGCAAGGAGTGCGTGTTCATTG AGAAGGTTCTGGAAAACAACTACACGGCCCTGATGTCTGCCAAGTACTCTGGTTGGTATGTGGGCTTCAC CAAGAAGGGGCGGCCTCGCAAGGGTCCCAAGACCCGCGAGAACCAGCAAGATGTACACTTCATGAAGCGT TACCCCAAGGGACAGGCCGAGCTGCAGAAGCCCTTCAAATACACCACAGTCACCAAGCGATCCCGGCGGA TCCGCCCCACTCACCCCGGCTAGGTCCGGCCACACTCACCCCCCCAGAGAACTACATCAGAGGAATATTT TTACATGAAAAATAAGGAAGAATCTCTATTTTTGTACATTGTGTTTAAAAGAAGACAAAAACTGAACCTA AAGTCTTGGGAGGAGGGGCGATAGGATTCCACTGTTGACCTGAACCCCATGACAAAGGACTCACACAAGG GGACC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 101004. Forward Primer - name:101004_F_cDNA_Fgf18, sequence:CTTTCTACTGCTGTGCTTCCAG; Reverse Primer - name:101004_N_SP6_cDNA_Fgf18, sequence:GGTCCCCTTGTGTGAGTCCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_010192 same experiment
	EMAGE:31969 same embryo
	EMAGE:31967 same embryo
	EMAGE:29795 same embryo
	EMAGE:29794 same embryo
	EMAGE:29797 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source