EMAGE mouse gene expression spatial database

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EMAGE:31964

Eps8l2 ( MGI:2138828)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004756_01	euxassay_004756_02	euxassay_004756_03	euxassay_004756_04	euxassay_004756_05

euxassay_004756_06	euxassay_004756_07	euxassay_004756_08	euxassay_004756_09	euxassay_004756_10

euxassay_004756_11	euxassay_004756_12	euxassay_004756_13	euxassay_004756_14	euxassay_004756_15

euxassay_004756_16	euxassay_004756_17	euxassay_004756_18	euxassay_004756_19

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

oral epithelium

moderate

regional

moderate expression: see section 03 05 06 07 08 09 10 11 12 13 14 15 16 17

inner ear

moderate

regional

moderate expression: see section 16 17 18 weak expression: see section 01 02 03 04 05 13 14 15

stomach

strong

regional

strong expression: see section 01 02 03 04

metanephros

moderate

regional

moderate expression: see section 01 02 03 04 05 not examined expression: see section 10

midgut

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14

thyroid gland

weak

regional

weak expression: see section 09 10 not examined expression: see section 11

conjunctival sac

moderate

regional

moderate expression: see section 01 02 04 19

choroid invagination

moderate

regional

moderate expression: see section 06 07 08 14 15 16 weak expression: see section 13

adenohypophysis

moderate

regional

moderate expression: see section 07 08 09 10 11

submandibular gland primordium

moderate

regional

moderate expression: see section 05 11 12 13

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 17 18 19

trachea

moderate

regional

moderate expression: see section 06 07 08

thymus primordium

weak

regional

weak expression: see section 06 08

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 09 10 weak expression: see section 08 12

vibrissa

moderate

regional

moderate expression: see section 18 weak expression: see section 03 04 19

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 06 14 weak expression: see section 07 08 09 12 13

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

naris

strong

regional

strong expression: see section 09

liver

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

4th ventricle

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 14 15 16 weak expression: see section 12 13

pharynx epithelium

strong

regional

strong expression: see section 06 07 08 moderate expression: see section 09 10

lung

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5999
Entity Detected:	Eps8l2, ( MGI:2138828)
Sequence:	sense strand is shown >T5999 GCGGACAAGCTGACCGAGGGGCCTAACCAAGCACAGGCGCTGAGGGACGGAGCGCAGGTCTGCCCTCCCT GACACTGGCTACCACTGGGAACGTCATGAGCCAGTCAGCATCCATGAGCTGCTGCCCAGGAGCTGCCAAC GGCAGTCTGGGCCGGTCTGATGGTGTGCCCAGGATGAGCGCAAAGGACCTGTTTGAGCAGAGGAAGAAGT ACTCCAACTCCAACGTCATCATGCATGAGACTTCACAGTATCACGTGCAGCACCTGGCCACATTCATCAT GGACAAGAGTGAGGCTATTGCCTCCGTGGATGACGCCATCCGGAAGCTGGTACAGCTGAGCTCCAAGGAG AAGGTCTGGGCCCAGGAAGTCCTGCTGCAGGTGAACGACAAGTCACTGCGACTGCTGGATGTTGAGTCCC AGGAAGAGCTGGAGAACTTTCCGCTGCCCACTGTGCAGCACAGCCAGACAGTGCTAAACCAGCTGCGCTA CCCCTCTGTGCTGCTGCTGGTGTGCCAGGACTCAGACCAGAACAAGCCCGACATCCACTTCTTTCACTGT GACGAGGTGGAGGCGGAACTTGTGCAGGAAGACATTGAGAGTGCTCTGGCTGACTACCGGCTGGGGAAGA AGATGAGACCACAGACCCTAAAGGGACACCAGGAGAAGATCCGCCAGCGGCAGTCTATCTTACCCCCTCC ACAGAGCCCAGCCCCCATTCCCTTCCAGCGACAGCCAGGAGATTCCCCTCAGGCCAAGAACCGTGTGGGC CTGCCCTTGCCAGTGCCATTCAGTGAACCAGGCTATCGCAGGAGGGAATCCCAGGACGAGGAGCCACGGG CTGTGCTGGCTCAGAGAATAGAGAAGGAGACGCAAATCCTTAACTGCACCCTGGATGATATTGAATGGTT CGTGGCCAGGCTGCAGAAGGCAGCTGAGGCTTTCAAGCAGTTGAACCAGCGGAAAAAGGGGAAGAAGAAG AACAAGAAGGGGCCAGCAGAGGGTGTCCTTACACTTCGGGCACGCCCCCCCTCGGAGGGAGAGTTTGTTG ACTGTTTCCAGAAAACCAAGCTGGCCATCAACCTGCTGGCGAAGCTGCAGAAGCACATCCAAAACCCTAG TGCAGCCGAGCTGGTGCACTTCCTCTTCGGGCCTCTGGATCTGATCATCAACACATGTGGCAGCCCCGAC ATAGCACGCTCTGTCTCCAGCCCTTTGCTGTCCACTGACGCCGTGAGCTTCTTACGTGGCCACTTGGTCC CCAAAGAGATGACACTGTGGGAATCACTGGGGGAGACCTGGATGAGGCCTCGCTCTGAATGGCCCCGGGA GCCACAGGTGCCACTCTATGTGCCCAAGTTCCGCAGCGGCTGGGAACCACCCCTGGATGTCCTACAGGAG GCTCCCTGGGAGGTAGAAGGACTGGCATCTGTGCCCAGTGATCAGCTGACCCCCAAGAATCGACTATCTG TACGACATTCCCCAAAGCACAGCCTCTCCTCCGAGTCCCAAGCCCCAGAGGACATTGCACCACCAGGCAG CTCCCCACATGCTAACAGGGGCTACCAGCCAACACCAGCCATGACCAAGTACGTCAAGATCCTCTATGAT TTCACAGCGCGAAACGCCAATGAGCTATCTGTGCTCAAGGACGAGGTCCTAGAGGTGCTGGAGGACGGCC GGCAGTGGTGGAAGCTACGAAATCGCAGTGGCCAGGCGGGCTATGTGCCCTGCAACATCCTGGCTGAAGC TCGGCAGGAGGACGTGGGTGCCCCCTTGGAACAGTCTGGCCAGAAATACTGGGGTCCAGCCAGCCCCACC CACAAACTGCCCCCAATTTTCGCAGGGAACAAAGAAGAGCTAATCCACCACATGGACGAGGTCAATGACG AGCTCATGAAAAAGATCAGCCACATCAAGACACAGCCGCAGCGCAACTTCCGCGTGGAGCGCAGCCAGCC AGTGCACCTGCCGCTTACCTTCGAGTCAGGTCCGGATGAGGTCCGCGCCTGGCTGGAAGCCAAGGCCTTC AGTGCCAGGATCGTGGAGAACCTGGGCATCTTAACTGGACCCCAGCTCTTCTCCCTCAACAAGGAGGAAC TGAAAAAAGTGTGCGGGGAAGAGGGCAGTCGCGTGTACAGCCAGCTCACGGTTCAGAAGGCCTTCCTGGA GAAACAACAAAGTGGGTCAGAACTGGAGAAGCTCATGAGCAAGATCAGGAGGGCCGAGGACAGCTATACC AGCCAACACACTTCACCAGAGTCAGAGGGGGCGCCGCACCTGTAATGGGCCCAACCCAAATGCATGGAGT ATTATTTTTATATGTGTATTATTTTATACCAAGGACATAGATGGGCTGGGTGTAACTGGCTTACCTGCCC TCCAGGCAGAGTCCTGTGTATGAGCCTAGCTACACCTGTGGGTAGCTCTTTTACCACCCGAGCCCTACCC CCAACCCAATACTCTTCTTCCAAAACCAAGGCCAGCCTCAGCCAAACCTGTACCTGGCAGTGCCAGTCCC TTCTCAACCTGCTGCCAAGGCCCCAGGACTCTCCCGGTGGGCAAGGCCTCTTCCCCTGGTCTTCTTGGTG CACATGAAGGTCCTTGTTCCTTACAATGCTGGGCCCCGACTCTGCAGCTCTCCCTTGTGTTGCTTGTTTC TACATTCACGTTTTCCTGCATCCGCTGTACCCCAATTGCAGGATGGGCCTGAGCCCTCAGCTGACCAGCC TGGTGTCCTTGCCTACATATTTGCCCACTCCCTTCACGCTGCTAGCATCCCAGGAACAACCTGGTCAGGG GCTCCAGGACAGGAGGCCTTGGGTGACCTGCTGGTATAGAGTATGCTGTATTCTTTCTTCTTTGAGACCT AAGAATGAACCAGACATAGGTTCTGACTTCAAATGTCCCATCCCACCCAAAGAGCCTGTGATAACAGATG TCTCTGGCTATTTCCACCAGCCTGTTCGCTGGTGTCTGGTGCGTTTCCCAGCTCTGGATGGAGCCCCAGA CCCAGAGATGAGAGTGGGGACTCCACAGCTATGTCAATAAACCAGATGTCTCCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3487337 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3487337 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004756 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source