EMAGE mouse gene expression spatial database

EMAGE:31967

Fech ( MGI:95513)
TS23 (14.5 dpc)
in situ hybridisation

Data Images

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

metencephalon part of 4th ventricle choroid plexus

strong

single cell

strong expression: see section 06 07 08 09 10

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

mesenchyme

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

liver and biliary system

strong

single cell

strong expression: see section 06

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36375
Entity Detected:	Fech, ( MGI:95513)
Sequence:	sense strand is shown >T36375 GAGAACATCAGAAGAGCGGAGTCTCTTAATGGAAATCCATTGTTCTCCAAGGCCCTGGCTGATCTGGTGC ACTCCCACATCCAGTCCAACAAGCTGTGCTCCACGCAGTTGAGTCTGAACTGTCCGCTCTGTGTAAATCC TGTCTGCAGGAAGACTAAATCCTTCTTCACCAGCCAACAGCTGTGACCCTGCCTGAGGACCCGTGGGATT TGCAGATGTGCAGCCTCCAGACACCTGCGGTGGGGACGATGGGGACAGAAGCCACGTTTGTCTGGGCACA AACTGTGATTTCTCTCTTGCCTGTGTTTTATCTTATTTTATCTTATTTCTCGCTTGCCCTATGTAGGCCA AACTGGCTTCCAGTGTGCCTCCCGCTCCCGTTTCCTGGGCGCTAGTTAGCATCACAGACATACAACATTG TGTTCCTTGGAGGATAGACTCGAAATTCTTACTGAGATTTCTATTTTCATATACAAGTATAGTGACTGAT CCTGGGGTCAATTTATCAATATGGAATATCTGTCTACTGGGACATCCCAGGAATGAGTTTCACAGTTTTA TTTTATGAGTCGCACACAAATTCTACACT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89101. Forward Primer - name:089101_F_cDNA_Fech, sequence:GAGAACATCAGAAGAGCGGAGT; Reverse Primer - name:089101_N_SP6_cDNA_Fech, sequence:AGTGTAGAATTTGTGTGCGACT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_010182 same experiment
	EMAGE:31969 same embryo
	EMAGE:29795 same embryo
	EMAGE:29794 same embryo
	EMAGE:29797 same embryo
	EMAGE:31952 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source