EMAGE mouse gene expression spatial database

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EMAGE:31969

Fgd3 ( MGI:1353657)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_010184_01	euxassay_010184_02	euxassay_010184_03	euxassay_010184_04	euxassay_010184_05

euxassay_010184_06	euxassay_010184_07	euxassay_010184_08	euxassay_010184_09	euxassay_010184_10

euxassay_010184_11	euxassay_010184_12	euxassay_010184_13	euxassay_010184_14	euxassay_010184_15

euxassay_010184_16	euxassay_010184_17	euxassay_010184_18	euxassay_010184_19	euxassay_010184_20

euxassay_010184_21	euxassay_010184_22	euxassay_010184_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15

facial vii ganglion

moderate

regional

moderate expression: see section 02 03 04 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 15 16 17 18 19 20 weak expression: see section 08

telencephalon mantle layer

weak

regional

weak expression: see section 09 16 17 18 19 20 21

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 15

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36377
Entity Detected:	Fgd3, ( MGI:1353657)
Sequence:	sense strand is shown >T36377 GAAGAGGACATCGTTAACCCAGCCAATGAGCTGATCAAGGAGGGCAGCATCCAGAAGTTATCAGCCAAGA ATGGCACCACTCAGGACCGACACCTCTTCCTGTTCAACAATGTGATGCTCTACTGTGTACCCAAACTGAG GCTCATGGGCCAGAAGCTAAGTGTACGGGAAAAAATGGACATCTCTGATCTCCAGGTACAAGATATTGTG AAACCAAACGCAGCCTGCACGTTCATCATAACAGGAAGAAAAAGGTCCCTGGAGCTTCAGACTCGGACAG AAGAAGAGAAGAAAGAATGGATTCAGGTCATTCAGGCCACTGTGGAGAAGCACAAACAGAAGAGTGAGAC CTTCAGGGCCTTTGGCGGTGCCTGTAGCCAGGATGAGGAGCCCACCCTGTCTCCAGACCAGCCCGTCATG AGTACCAGCTCTGTGGAACCTGCAGGGGTAGCTGACAGCAATGGGGGTACTCCCGGGATCGAGTCCAGAA AGTCGTCCTCTAAAACAAGACGCGACAAAGAGAAGCCCGGGTGTAAGAGCTGTGGTGAGACCTTCAACTC CATCACTAAGAGGAGGTATCGCTGCAAGCTGTGTGGAGAGGTCATCTGTAGGAAGTGCTCAGAGTTCAAG GCAGAGAACAGTAAGCAGAGCCGCGTCTGCCGAGAGTGTTTCCTGGAAGAGCCCCTGGTCCCTCCAAGCC CCAGCTCTGAGACTCCCACTGAGCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 99974. Forward Primer - name:099974_F_cDNA_Fgd3, sequence:GAAGAGGACATCGTTAACCCAG; Reverse Primer - name:099974_N_SP6_cDNA_Fgd3, sequence:AGCTCAGTGGGAGTCTCAGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_010184 same experiment
	EMAGE:31967 same embryo
	EMAGE:29795 same embryo
	EMAGE:29794 same embryo
	EMAGE:29797 same embryo
	EMAGE:31952 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source