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EMAGE:31977

Thbs4 thrombospondin 4 ( MGI:1101779)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_000366_22 euxassay_000366_01 euxassay_000366_02 euxassay_000366_03 euxassay_000366_04
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euxassay_000366_10 euxassay_000366_11 euxassay_000366_12 euxassay_000366_13 euxassay_000366_14
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
esophagus
weak weak
regionalweak expression: see section 13 14 15
diaphragm
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 20 21 22 weak expression: see section 01
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
tail
moderate moderate
regionalmoderate expression: see section 13 14 17 18
limb
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07
tongue
weak weak
regionalweak expression: see section 11 12 13 14 15 16 17 18
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1671
Entity Detected:Thbs4, thrombospondin 4 ( MGI:1101779)
Sequence:sense strand is shown

>T1671
TGGCCTCGAGGNCAGATTCGGACGAGGCGTAAACACCCAGACAGATGATGACTATGCTGGCTTTATTTTT
GGTTACCAAGACAGCTCCAGCTTCTATGTAGTCATGTGGAAGCAAACGGAGCAGACATACTGGCAGGCTA
CACCGTTCCGTGCAGTAGCAGAACCTGGTATTCAGCTCAAGGCTGTGAAGTCTAAAACTGGCCCAGGGGA
ACATCTCCGAAACTCCCTGTGGCACACAGGGGACACCAGCGACCAGGTCAGGCTGCTGTGGAAGGACTCC
AGGAATGTGGGCTGGAAGGACAAGGTCTCCTACCGCTGGTTCCTGCAGCATAGGCCCCAGGTGGGCTACA
TCAGGGTGCGCTTTTATGAAGGCTCGGAGTTGGTGGCTGACTCTGGGGTCACCATAGACACCACCATGCG
TGGAGGCCGACTTGGTGTGTTCTGCTTCTCTCAAGAAAACATCATCTGGTCCAACCTCAAGTACCGCTGC
AACGACACCATACCAGAGGACTTCCAGGAGTTCCAGACCCAGAGTTTCGACCG
Notes:The probe template was PCR amplified from IMAGE:473150 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:473150 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000366 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS