EMAGE mouse gene expression spatial database

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EMAGE:31991

Csdc2 cold shock domain containing C2, RNA binding ( MGI:2146027)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002780_01	euxassay_002780_02	euxassay_002780_03	euxassay_002780_04	euxassay_002780_05

euxassay_002780_06	euxassay_002780_07	euxassay_002780_08	euxassay_002780_09	euxassay_002780_10

euxassay_002780_11	euxassay_002780_12	euxassay_002780_13	euxassay_002780_14	euxassay_002780_15

euxassay_002780_16	euxassay_002780_17	euxassay_002780_18	euxassay_002780_19	euxassay_002780_20

euxassay_002780_21	euxassay_002780_22	euxassay_002780_23	euxassay_002780_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 16 17 18 19

facial vii ganglion

strong

regional

strong expression: see section 03 moderate expression: see section 20 21

not examined

regional

not examined expression: see section 01 02 03 23 24

neural retina

strong

regional

strong expression: see section 01 02 03 23 24

vagus x ganglion

moderate

regional

moderate expression: see section 08

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 moderate expression: see section 05 06 07 08 17 18 19 20 21 22

cervical ganglion

moderate

regional

moderate expression: see section 09 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 16 17

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18

thoracic ganglion

moderate

regional

moderate expression: see section 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2174
Entity Detected:	Csdc2, cold shock domain containing C2, RNA binding ( MGI:2146027)
Sequence:	sense strand is shown >T2174 AATTCGGATCCACGTGCCCTGAGAAATGGCTGCAAGCAGGAGGGAACTTTAGCACTCCTGTTATGTAGGC CAGTCCTCTCTCCTCAGCTGTCAGATGATCTTTGAACCCCACAGAGCTGAGGAAGCCAAGCTCACTCACT CCCTGAGGTGAGGCGGGGATAGGGTTTCCTTCCAGCTTGCTCTTCTACCCGGGATGCTCAAACCTCACGA ATGCTCTGGCCCATTGCTGGCCATGACAGCAGCTGAGGATGCAGCAATGGACTGACATGTCACCTCAGGC AAGGCCAGGTCCAGGCACCCAAAGGATCGGTCCTCAGCCAGGGAGACCACTGGCTTTGCCCCTCCCACCC CCCATGAGATCCTCTTCCCAGAGCTATGCAATGGCTCTCATAAGCCCCTGACAGGTCAGCAGCTGAGGTG GCTCTTATCTCTCTTGTCCCCAAGTGTGACTGTCCCTCTTCCCTGGAGTCCCTTTGGAGCTGTGTCCCTT CTAGAGAGCCAGTTACTTAACCAGAGGATAAGTGAGGTCACA
Notes:	The probe template was PCR amplified from IMAGE:889232 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:889232 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002780 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source