EMAGE mouse gene expression spatial database

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EMAGE:32000

Mphosph6 ( MGI:1915783)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_003644_16	euxassay_003644_17	euxassay_003644_18	euxassay_003644_19	euxassay_003644_20

euxassay_003644_21	euxassay_003644_22	euxassay_003644_23	euxassay_003644_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

right lung

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20 21 22 23 24

orbito-sphenoid

moderate

regional

moderate expression: see section 05 22 23 weak expression: see section 01 02 03 04 06 24

testis

moderate

regional

moderate expression: see section 06 07 09 22 23 weak expression: see section 08 24

left lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6843
Entity Detected:	Mphosph6, ( MGI:1915783)
Sequence:	sense strand is shown >T6843 CGGACGCGTGGGGTGCGTCCAACATGGCGTCGGAGCGCAAGACTAAGTTATCCAAGAACCTGCTGCGCAT GAAGTTCATGCAAAGAGGCCTGGACTCGGAAACCAAGAAACAGCTAGAAGAGGAGGAGCGGAAGATGATC AGCGACGAGCACTGGTACCTGGATTTGCCGGAGTTGAAGGAGAAGGAGAGCTTCATCGTAGAGGAGCAGA GCTTCTCCCTGTGTGAAGATCTTCTCTACGGAAGGATGTCATTCAGGGGGTTTAATCCTGAGGTTGAGAA GTTAATGCTTCAGATGAATTCTAAGAACCGAGCAGAGGCAGCAGAGGAAGACGAGACGGTAGAGGTCGAT GTGTCTGATGAGGAAATGGCAAGAAGATATGAGACTTTGGTGGGAACAATTGGAAAAAAGTTTGTCAAGA AAAGGGACCGAGCCAATTACGAAGAAGATGAAAATGGAACTATAAAAGCCATAAAACCTAAGAAAATGTT CTTAAAGCCCCAAGACTAAAATGGATGCCTCCGATACAGGGGATGGCTGTGAGCACAGCATGGTCGGAAC TCATCCTCCTGTCGTCGGTACTGGTTAAAGCACCGTTTCCCTCTAAAGAAATGCATAAGTTTGGAGATGT GCTGTTCTTGAGACAGATGTGTAATGGATGTTATACATCCTTTTTATGACATTCTCAGGGGCTTCAGATA TGTGGGCACAGGCCGCTCCCTAGATTTTTGACCTGATTCTGTGTGTATATACCCACAGACAGGCCACATG CACACCGTTCTCCAGTTACTGTCCAGGAATGACAATTGTAAACTAATTATTGCCTTTGTTTAACTGCCCT TATATTTATATTTATATTTTTTTGGATCATGTCTCTCCAGTTCACTTTGGAGTCACATCTTTGAGAACTT TCCAGAAGTGATTTGTCAGGAACAGACATGGCTCTCGGGCCTTTGTCGTGTTGAGTAGTGTGGGGTTTGT GTATTTTAAACAGTCATCTGGGTCATTTTTCTAATGTTCTATTAATAAACTTATACTTTTCTGTTTTCTT AAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3964232 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3964232 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003644 same experiment
	EMAGE:30649 same embryo
	EMAGE:30657 same embryo
	EMAGE:32029 same embryo
	EMAGE:30656 same embryo
	EMAGE:30697 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source