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EMAGE:32012

Fbxl22 F-box and leucine-rich repeat protein 22 ( MGI:1921415)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:32012 EMAGE:32012 EMAGE:32012 EMAGE:32012 EMAGE:32012
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euxassay_002795_21 euxassay_002795_22 euxassay_002795_23 euxassay_002795_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
esophagus
moderate moderate
regionalmoderate expression: see section 13 14 15
vertebral axis musculature
weak weak
regionalweak expression: see section 02 03 04 05 06 07 08 09 10 17 18 19 20 21 22 23 24
hindgut
strong strong
regionalstrong expression: see section 11 12 13 14 15
foregut-midgut junction
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16
stomach
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09
tongue
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15
rectum
strong strong
regionalstrong expression: see section 13 14
midgut
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
bladder
strong strong
regionalstrong expression: see section 11 12 13 14 15
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3649
Entity Detected:Fbxl22, F-box and leucine-rich repeat protein 22 ( MGI:1921415)
Sequence:sense strand is shown

>T3649
TGGCCTCGAGCCAGATTCGGACGAGGGAAGCCGGAGGAGCCTCTCCAGGACTTGCTCCCAGCTCCGAGAT
GTGTTTGAGGACCCCACCCTCTGGCCCCTGCTGCACTTCCATTCTCTCGCAGAGCTCAAGAAAGACAACT
TCCGGCTGAGCCCTGCGCTGCGCAGCCTGTCCATCTGTTGGCATTCCAGCCGTGTGCAGGTGTGCAGCAT
CGAGGACTGGCTCAAGAGCGCCCTCCAGAGGAGCATCTGCAGCCAGCACGAGAGCCTGGTTAATGATTTC
CTCCTGCAGGTGTGCAACAGGTGCCCCAACCTGACGTCCGTCACGCTGTCGGGCTGCGGCCACGTCACGG
ACGACTGCCTGGCGCGCTTGCTGCTCAGCTGCCCGCGCCTGCGTACGCTGCGCCTCGAGAACTGCGCGCG
CGTTACCAACCGCACGCTGGCGGCCGTGGCCGCGCACGGGCGCGCGCTGCAGACTCTGCACGTGGACTTC
TGCCGCAACGTGAGCGCCGCCGGCCTGCTCCGCCTCCGCGCCGCCTGCCCGAACCTGCGCC
Notes:The probe template was PCR amplified from IMAGE:348445 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:348445 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002795 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS