EMAGE mouse gene expression spatial database

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EMAGE:32013

Dnmt3b DNA methyltransferase 3B ( MGI:1261819)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002794_01	euxassay_002794_02	euxassay_002794_03	euxassay_002794_04	euxassay_002794_05

euxassay_002794_06	euxassay_002794_07	euxassay_002794_08	euxassay_002794_09	euxassay_002794_10

euxassay_002794_11	euxassay_002794_12	euxassay_002794_13	euxassay_002794_14	euxassay_002794_15

euxassay_002794_16	euxassay_002794_17	euxassay_002794_18	euxassay_002794_19	euxassay_002794_20

euxassay_002794_21	euxassay_002794_22	euxassay_002794_23	euxassay_002794_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 07 08 19 20 moderate expression: see section 09 10 18 21

thymus primordium

strong

regional

strong expression: see section 13 14 15 16 17

renal cortex

moderate

regional

moderate expression: see section 08 19 20 weak expression: see section 09 10 11 12 21 22 23

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 moderate expression: see section 07 08 17 18 21 24 weak expression: see section 09 10 11 15 19 20 22 23

midbrain ventricular layer

strong

regional

strong expression: see section 16 17 moderate expression: see section 11 12 13 15 weak expression: see section 14 18

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 06 20 21 22 23 24 weak expression: see section 07 08 18 19

axial musculature

strong

regional

strong expression: see section 23 24 moderate expression: see section 06 07 08 09 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2181
Entity Detected:	Dnmt3b, DNA methyltransferase 3B ( MGI:1261819)
Sequence:	sense strand is shown >T2181 TGGCCTCGAGCCAGATTCGGCACGAGGGATCGCTTCCTAGAGCTCTTCTACATGTATGATGAGGACGGCT ATCAGTCCTACTGCACCGTGTGCTGTGAGGGCCGTGAACTGCTGCTGTGCAGTAACACAAGCTGCTGCAG ATGCTTCTGTGTGGAGTGTCTGGAGGTGCTGGTGGGCGCAGGCACAGCTGAGGATGCCAAGCTGCAGGAA CCCTGGAGCTGCTATATGTGCCTCCCTCAGCGCTGCCATGGGGTCCTCCGACGCAGGAAAGATTGGAACA TGCGCCTGCAAGACTTCTTCACTACTGATCCTGACCTGGAAGAATTTCAGGAGCCACCCAAGTTGTACCC AGCAATTCCTGCAGCCAAAAGGAGGCCCATTAGAGTCCTGTCTCTGTTTGATGGAATTGCAACGGGGTAC TTGGTGCTCAAGGAGTTGGGTATTAAAGTGGAAAAGTACATTGCCTCCGAAGTCTGTGCAGAGTCCATCG CTGTGGGAACTGTTAAGCATGAAGGCCAGATCAAATATGTCAATGACGTCCGGAAAATCACCAAGA
Notes:	The probe template was PCR amplified from IMAGE:891000 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:891000 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002794 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source