EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:32024

Casp8ap2 caspase 8 associated protein 2 ( MGI:1349399)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002768_01	euxassay_002768_02	euxassay_002768_03	euxassay_002768_04	euxassay_002768_05

euxassay_002768_06	euxassay_002768_07	euxassay_002768_08	euxassay_002768_09	euxassay_002768_10

euxassay_002768_11	euxassay_002768_12	euxassay_002768_13	euxassay_002768_14	euxassay_002768_15

euxassay_002768_16	euxassay_002768_17	euxassay_002768_18	euxassay_002768_19	euxassay_002768_20

euxassay_002768_21	euxassay_002768_22	euxassay_002768_23	euxassay_002768_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 09 10 11 17 18 19 20 21

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 11 16 17

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17 18

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T822
Entity Detected:	Casp8ap2, caspase 8 associated protein 2 ( MGI:1349399)
Sequence:	sense strand is shown >T822 TCCTCGAGNCTGTTGGCCTACTGGGTATACAGCTCAGAAAAGAGAAGCCCTGCATCTCTTCCATACTCTT AGAAGATCTTGCGGTCTCTTTAACAGTACCGTCACCTCTGAAATCAGATGGCCATTTGAGTTTCTTAAAG CCAGAAGTTTTGTCAACTTCAACTCCTGAAGAAGTTATTAGTGCACATTTTAGTGAGGATGCTTTGCTTG AGGAAGAGGATGCATCTGAACAGGACATTCATCTAGCTCTGGAGTCTGATAACTCAAGCAGTAAGTCAAG CTGTTCATCATGGACAAGCCGGTCTGTTGCTTCAGGCTTTCAGTACCACCCTAATCTTCCCATGCATGCT GTCATAATGGAAAAGTCCAATGATCATTTCATTGTGAAAATACGGCGTGCAACACCATCTACCTCCCCTG GCCTTAAACATGGTGTGGTAGCTGAGGAGTCATTGACATCTTTGCCTAGAACTGGAAAAGAAGCTGGTGT AGCAACAGAGAAAGAACCTAACCTGTTTCAGAGTACAGTTTTAAAACCTGTC
Notes:	The probe template was PCR amplified from IMAGE:1922175 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1922175 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002768 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source