EMAGE mouse gene expression spatial database

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EMAGE:32042

1700065O13Rik ( MGI:1920701)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004940_01	euxassay_004940_02	euxassay_004940_03	euxassay_004940_04	euxassay_004940_05

euxassay_004940_06	euxassay_004940_07	euxassay_004940_08	euxassay_004940_09	euxassay_004940_10

euxassay_004940_11	euxassay_004940_12	euxassay_004940_13	euxassay_004940_14	euxassay_004940_15

euxassay_004940_16	euxassay_004940_17	euxassay_004940_18	euxassay_004940_19	euxassay_004940_20

euxassay_004940_21	euxassay_004940_22	euxassay_004940_23	euxassay_004940_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 12 13 14 15 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 05

vagus x ganglion

moderate

regional

moderate expression: see section 06 07 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21

cervical ganglion

moderate

regional

moderate expression: see section 07 08 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 09 14 15 16

spinal cord

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 17

thoracic ganglion

moderate

regional

moderate expression: see section 10 11

retina

moderate

regional

moderate expression: see section 01 02 03 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7989
Entity Detected:	1700065O13Rik, ( MGI:1920701)
Sequence:	sense strand is shown >T7989 GACAAGAGGTAATCCTGGGGCTGGACTATATCTCCTGTATTTAGGCCTATTAGGAAGAACACCTGGATGC ATATGAAAGAGGAAATGGAGCCTGTGACCTTTGAGGATGTGGCTGTGAACTTCAGTCCAGGAGAGTGGGC TCTGTTGGATTCCAGTCAAAAGAAGCTCTACAGAGATGTGATGACAGAAACCTTTATGAACCTGATCTCC ATAGGGAAAACAGAAGAAAACATTGAAGAGGACTATGCTAATGTCAGAAGTCTGAGAACTCAGATGATTG GGAGAAATTGTAAATATGAAGATAGCTTTCAGCGTGGAGAAGCCCACCACCGAATTCCAGAATATGTTGT TAACAAGGGCATGCCTTCTGCAACAGCAGTGTATGAAAGCAATGTGTGGGCAAGAGGCATCCGTGCTCAC TCCCCTTCACGTGTGCATCTCACAGATGAAACTGGAGCGAAACCATTTGAGAATAAGGAACATGTGCATG TAGCTTTTCAGTATGAGACACGTTATGCAGATGTCACTTCTTCTGAGCCCTCTCACATACTAGAAGGTCC CAGAGAGATAGTTTATCAAAAGAAACAGCCTAATGAAGCCTATAGGATTCTCACTTCTGATCAAAATTAT GAGAGAATTGACAGTGAAGGTACATATAATCAGTTTGATAAAGCCATCCAGAGATATGCTTATTTTCAGA TGTATGAAAAACTCCACACTAGAGTGAAGCCATTTATGTGTAAGCAATGTGGGGAAGCCTTTGTTCATTT CAGCCAACTTGTCTACCATGAAAGAATTCATACAGGAGAGGAATGTTATGTGTGTAGTCAGTGTGGAAAA GCATTTAGACACTATTCATACCTCCAGAAACATGAAAGAATTCACAGTGGAGATACACCTTATGTGTGTA AGCAGTGTGGCAAAGCCTTTGTTCGTTCCTCACACCTTCTTGTCCATGAAAGGACTCACACTGGAGAGAA ACCTTATACATGTAAGCATTGTGGGAAAGCCTTCAGTCATCACAGTGCCTGCTACAGACACGAAGTAATT CACACTGAAGAGAAACCCTATGTCTGTAAGCAGTGTGGGAAGGCATTCACTTGTTCTACATCCCTTCGCA ATCACGGAAGAATTCACACTGGAGAAAAACCTTACGCATGTAAGCAGTGTGGCAAATCCTTCATCCAACG AGATGCTTGCTACAATCACGAAAGAACTCATACTGGAGAGAAGCCCTATGTGTGTAAGCAGTGTGGGAAA GCATTTACATGGTCCACATGCCTTCGCAACCATGAAAGGACTCACACTGGAGAGAAACCTTACACATGTA AGCATTGTGGGAAAGCCTTCACCCATTCCAGTACCCGGTATAGTCATGAAAGAATTCATACTGGAGAGAA ACCATACGTATGTAAAAAGTGTGGCAAGGCATTCATTCATTCCAGTCATCTTATCAGCCATGAAAAGATC CACACTGGAGAGAAACCGTATCCATGCAAGCATTGTGGGAAAGCCTTCATTCAACGGAAGGCGTGTCACA ATCATGAAAGAACTCATACTGGAGAGAAACCCTATGCATGTAAATATTGTGAAAAAGCATTTAGGTGTTC CACATACCTTCGGAACCATGAAAGAACTCACGCTGGAGAGAAACCTTATATATGTAAGCATTGTGGAAAA GCCTTTACACGTCAGAGTGCCCGTTACCGTCATGAACGAATTCACACTGGAGAGAAACCCTACGTGTGTA AAAAATGTGGGAAAGCATTCAGTGATTGTAGCTACCTTGTGAAGCATGAAAGAATCCATTGGAGAGAAAC TTTACACATGTAAGAATCAAGAAGCTTTTATGTGTTCCACATTCCTTTATACACACAAAAGAGCTGACAG AGAAGAGCTGAGAACTTTACAGCAGTGTATCTGGTGGACTTGTCTGTGCACAACTTATATGCATGAAAAA AATGTGAGAACAGCTCACAAAGAGGCTCGGCGGGTAAAGGTGCTTGTTACTAAGCTTGAGAATCTGAGTT CCGTCCCAGGGACCCATATGGTAGAAATAGAAAATTAATCCCCCAAATTTACCTCTGAATTCCATGTATA CATGTGGACCCCCACACAACCCCATTATTTAAAGTGTATATACATATAGAGAACAATTGAAATTTTCAGA AGTTAGTTGTATCCCTGATATGCAAGCATATATAGGATAAAATCCTATGGCACTGGGCATGGTAGGATGT GCCTTTTATTCTAGCACTGGGCAAGGTGGGGATAGTAAAGAGTAGAGAGTTATAATGGATATAGTGGATT TGAACACTCCATGGGAAGTTTAGGATGAGCTAGTTACAAGCAAGCAGAATTTTCTTTGCAAGAAGAAAAG ACTAGAGAGACAGTTCAGCTGTTCAGAGCCCATGCTGCTCTGCCAGGGGGCCTGAGTTTGTCTCAGCATC AACATTGTGTGGCCTACAACCATCTAAGAACTGTGATGCCCTCTTCTGGCCTCTGTAGGGATAGAACACA TGGGATGCATATACATATAAATAAAATAAGTCTTTTTTTTTAAAAATTATGTGTACAAAGCAAATAGGAG AAACGTGTTTTTTCAAAAAATCTGAAGTATCATGTAGTCTAATCTTATAAGAATAAGAAATGTGGAGACA GCAAATTTATTTGCACAGGATATTTCTGGGGATAACCTGGAGTACAGTAAAATTGTGAGCATTTTTTCAT TGTCTGATTCTTACATGAGATCTTTAGTTTGCATTATTTTTTTTTTTTATATTTCTGATAGAAGATTTGG TGAATAATGTCATGATTTTGCCTGCCTATATTTCCTGGTTCTTGAACTGCTGAATCTAACACGACACTGT CTCTAGCAATACAATCACATCCATATAAAGAAACATTTGCATTGAGGTGATGTAAGCGTGATTAATTAAT TTCAGTTCAGTGGGTGTTGTTTAATGAAGTGAGGCAGTGCTTTTTCTACTTTACTGTAGAGCTGTTGGAG TCACTGAACTGAGATACATTTGTAATAAAACAGTAGACTTAATTTTATCTAGTTTGTTGGTAAATGTGGT TCATTTGAAAGTGAAACTGTAAGCTGCTTAAAAGTGCTTTCTCACTCTTACATGTCATTTTTTAAAAATA TTAACCCCTTTGCAATTTCCTTGCATTTATGTGAAAAATGGAATGTTTCTGGCATTTGTATAGATTTTAT ATTTTGTGCTTTTTAGTGTATGTGTTCTAATAAGTGTTTTGTTTCTTTTTTTGTTTGTAGATAGGATCTT ACTATGTATGCTTGGCTGGCCTAGAGCTTACATAGTTCCTGCCTTTGCCTCCAAAGAGTTGGAATTAAAA CTTACACTACCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30011543 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30011543 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004940 same experiment
	EMAGE:30055 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source