EMAGE mouse gene expression spatial database

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EMAGE:32052

Cldn9 ( MGI:1913100)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_002533_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14

pituitary gland

moderate

regional

moderate expression: see section 08 09 10 11 12 13

cochlea

strong

regional

strong expression: see section 04 05 06 07 14 15 16 17 18 19 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5061
Entity Detected:	Cldn9, ( MGI:1913100)
Sequence:	sense strand is shown >T5061 CAAGTGGCACCTCACGGTTCCCTGTTTTGAGACAAGCTGTATACCAGCTGAGAGAAGACTTCAACCAAGA AAGTACGTGAGCAGCCAGCAGAGAGGAACGCGGTTGTTCCTAGTTCATGGCAGATCTGGAGGGGCTGTAA TGGGTGAAGGCTTCCAGGAGGACACAAGCAATACAGATGAGCGGGACCTAAGGACTTCTTCGTATTCAGT GAGTACCAGATGTGTGAGAGGCCCGCAGCTGTGAGGTCTGGCCTGGTCTGAGATCAACAGATCCCCCTCC TGAGCAGTGAGACGCACCCGAACTCCAACACAGTGCTCCCAACCCTATTGAGTGATTCAGGCCAAGAGCT GAGAAGACCCGAGGAGCAGATGGCTTCCACTGGCCTTGAACTCCTCGGCATGACCCTGGCTGTGCTAGGC TGGCTAGGAACTTTGGTGTCCTGTGCCCTGCCACTGTGGAAGGTGACCGCCTTCATCGGCAACAGCATCG TTGTGGCCCAAGTGGTATGGGAGGGGCTGTGGATGTCCTGTGTGGTCCAGAGCACTGGCCAGATGCAGTG CAAAGTATACGACTCACTGCTGGCGCTGCCCCAGGACCTGCAGGCAGCCAGAGCCCTCTGTGTCGTGGCC CTCCTGCTGGCTTTGCTGGGCCTGCTGGTGGCTATCACGGGCGCCCAGTGCACCACGTGTGTGGAGGACG AAGGTGCCAAGGCCCGTATCGTACTCACCGCAGGGGTCCTCCTCCTCCTCTCGGGCATTTTGGTGCTCAT CCCTGTCTGCTGGACAGCCCATGCCATCATCCAGGATTTTTATAACCCACTGGTTGCGGAAGCCCTCAAG AGAGAACTGGGGGCTTCCCTCTACCTGGGCTGGGCTGCCGCTGCCCTGCTCATGCTAGGGGGAGGGCTCC TCTGCTGTACGTGTCCCCCGTCACACTTTGAGCGTCCCCGCGGCCCCAGGCTGGGCTACTCCATCCCTTC CCGTTCAGGTGCTTCGGGACTGGATAAGAGGGACTATGTGTGAGGCTGAGGCTTCTTCCAGAAGCTTCCA CCTGCGGCTTCATGCCCTGCATTGGGCTACATCCTTATATCATCAAATCCATGCGCCTGCGAAGCTCACT TATTGGCCAGGACTTGGCTCTTAGGGGATCTCAGCTGGTCTGGCTTGAGCTAACCCTCTGTAGTGGTTGC AACCTTGAGAAAGCTCCAGTTACTGGGTACCCTGCTTATCGCTGCCTCCCAGGATTTTGCTGTGGTTTGC TCTCCTGGACTTTCTTACTCTGGAACCTGGACCTCAGCCTCCTTGCCTTCAAAGTAAGATGTGGACTGTG ACCTAATACTGAGGCTTTAGCTGGTCAAGTCTAGCCCAAGCACCACCTGAATCCAAGTTACTCAGCCCCC TACCCTACCTGTGAATAAAAGCACATTGTAACTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5320961 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5320961 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002533 same experiment
	EMAGE:29456 same embryo
	EMAGE:29841 same embryo
	EMAGE:29455 same embryo
	EMAGE:29842 same embryo
	EMAGE:29812 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source