EMAGE mouse gene expression spatial database

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EMAGE:32072

Gpr98 ( MGI:1274784)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_010149_01	euxassay_010149_02	euxassay_010149_03	euxassay_010149_04	euxassay_010149_05

euxassay_010149_06	euxassay_010149_07	euxassay_010149_08	euxassay_010149_09	euxassay_010149_10

euxassay_010149_11	euxassay_010149_12	euxassay_010149_13	euxassay_010149_14	euxassay_010149_15

euxassay_010149_16	euxassay_010149_17	euxassay_010149_18	euxassay_010149_19	euxassay_010149_20

euxassay_010149_21	euxassay_010149_22	euxassay_010149_23	euxassay_010149_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

strong

regional

strong expression: see section 11 12 14 moderate expression: see section 03 04 05 06 07 08 09 10 15 16 17 18 19 20 21 22 23 weak expression: see section 02

midbrain ventricular layer

strong

regional

strong expression: see section 12 13 14 moderate expression: see section 09 10 11 15 16

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 12 13 moderate expression: see section 11 17

pons ventricular layer

strong

regional

strong expression: see section 12 13 moderate expression: see section 08 09 10 11 14 15 16

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 17 weak expression: see section 18 19 20 21

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 13 14

spinal cord ventricular layer

strong

regional

strong expression: see section 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T32065
Entity Detected:	Gpr98, ( MGI:1274784)
Sequence:	sense strand is shown >T32065 ATGAGCACACGGAGAGGCGGTGCCTGCTCTTCTGCCTTCTCAGCTGGGGGCTTCCATCTTTCGTGGTGAT TCTCCTCATACTTATTTTGAGAGGAATCTATCATCGGAGCATGCCGCAGATCTATGGACTTATTCATGGT GACCTATGCTTCATCCCAAACATCTATGCTGCTCTGTTCACGGCTGCTCTTGTTCCTCTAATGTGCCTCG TGGTGGTATTTGTGGTGTTCATTCATGCCTACCAGCTGAAGCCGCAGTGGAAAGGTTATGATGACGTCTT CAGAGGACGAACCAACGCAGCAGAAATTCCTCTGATTTTATATCTCTTCGCCCTGATTTCTATGACGTGG CTTTGGGGAGGACTGCATATGGCCTACAGACACTTCTGGATGTTGGTTCTCTTTGTCATTTTCAATAGTC TGCAGGGACTTTATGTTTTTGTGGTTTATTTCATTTTACACAACCAAACGTGTTGCCCTATGAAGGCCAG CTATACAGTAGAAATGAATGGTCACCCAGGACCCAGCACAGCCTTCTTCACTCCTGGGAGCGGAATACCT CCAGCTGGAGAGATAAACAAGTCTACCCAGAACCTCATCAATGCTATGGAAGAGGTGCCATCTGACTGGG AAAGATCATCCTTCCAGCAGACCAGCCAGGCAAGCCCTGATTTGAAGACAAGTCCCCAGAATGGTGCCTC ATTCCCTTCTTCTGGAGGTTATGGCCCGGGGTCCCTGATAGCCGATGAAGAGTCCCAGGAGTTTGATGAC CTGATATTTGCATTAAAAACTGGTGCTGGTCTCAGTGTCAGTGACAACGAATCTGGTCAAGGAAGCCAGG AAGGAGGCACCCTGACTGACTCCCAGATTGTAGAACTCAGAAGGATACCAATTGCTGACACCCACCTCTG AGAGTCCCATTTACACTCTCCAGTAGAAAGAGGACAAGTTTCCTCATTGCCTTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5050650), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 57870. Forward Primer - name:057870_F_IRAV89_c07_Mass1, sequence:ATGAGCACACGGAGAGGC; Reverse Primer - name:057870_R_SP6_IRAV89_c07_Mass1, sequence:GCCAAGGCAATGAGGAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_010149 same experiment
	EMAGE:32031 same embryo
	EMAGE:29846 same embryo
	EMAGE:29289 same embryo
	EMAGE:29288 same embryo
	EMAGE:31981 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source