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EMAGE:32081

Faah fatty acid amide hydrolase ( MGI:109609)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
foregut-midgut junction
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17 18
pituitary gland
moderate moderate
regionalmoderate expression: see section 13 16 17 weak expression: see section 11 12 14 15
vestibulocochlear viii ganglion
weak weak
regionalweak expression: see section 07 08 09 19
stomach
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11
kidney calyx
moderate moderate
regionalmoderate expression: see section 09 10 11 12 weak expression: see section 19 20 21
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 12 18 19
rectum
moderate moderate
regionalmoderate expression: see section 15
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 15 16
midgut
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 19 weak expression: see section 11 13 14
pancreas
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 17 18 19
submandibular gland primordium
weak weak
regionalweak expression: see section 09 10 18 19
vagus x ganglion
weak weak
regionalweak expression: see section 09 10 18 19
trigeminal v ganglion
weak weak
regionalweak expression: see section 04 05 06 07 08 09 10 18 19 20 21 22
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 09 10 11 12 15 16 17 18 19 moderate expression: see section 08
hindgut
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16 17 18 19
cervical ganglion
moderate moderate
regionalmoderate expression: see section 11 18 19
lung
moderate moderate
spottedmoderate expression: see section 09 10 11 12 13 14 17 18 19 weak expression: see section 06 07 08 20 21 22
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T924
Entity Detected:Faah, fatty acid amide hydrolase ( MGI:109609)
Sequence:sense strand is shown

>T924
TCCTCAGNCTGTTGGCCTACTGGGGATCGCGGGCGATCCAGGCCGGGTTTTGCAGCGGAGCTGTTGGTGT
GCGCGTGCCGAGTCCTCTCGGGTGGCGGTCGGCTGCAGGAGATCATGGTGCTGAGCGAAGTGTGGACCGC
GCTGTCTGGACTCTCCGGGGTTTGCCTAGCCTGCAGCTTGCTGTCGGCGGCGGTGGTCCTGCGATGGACC
AGGAGCCAGACCGCCCGGGGCGCGGTGACCAGGGCGCGGCAGAAGCAGCGAGCCGGCCTGGAGACCATGG
ACAAGGCGGTGCAGCGCTTCCGGCTGCAGAATCCTGACCTGGATTCAGAGGCCTTGCTGGCTCTGCCCCT
GCTCCAACTGGTACAGAAGTTACAGAGTGGGGAACTGTCCCCAGAAGCTGTGCTCTTTACCTACCTGGGA
AAGGCCTGGGAAGTGAACAAAGGGACCAACTGTGTGACCTCCTATCTGACTGACTGTGAGACTCAGCTGT
CCCAGGCCCCACGGCAGGGCCTGCTCTATGGCGTCCCCGTGAGCCTCAAGG
Notes:The probe template was PCR amplified from IMAGE:1925032 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1925032 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002770 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS