EMAGE mouse gene expression spatial database

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EMAGE:32091

Ssbp2 single-stranded DNA binding protein 2 ( MGI:1914220)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002180_01	euxassay_002180_02	euxassay_002180_03	euxassay_002180_04	euxassay_002180_05

euxassay_002180_06	euxassay_002180_07	euxassay_002180_08	euxassay_002180_09	euxassay_002180_10

euxassay_002180_11	euxassay_002180_12	euxassay_002180_13	euxassay_002180_14	euxassay_002180_15

euxassay_002180_16	euxassay_002180_17	euxassay_002180_18	euxassay_002180_19

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

midbrain

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

diencephalon

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 11 12 13 14 15

telencephalon marginal layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 11 15 16 17 18 19

hindbrain

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16

spinal cord

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12

foot mesenchyme

moderate

regional

moderate expression: see section 01 02 03 14 15 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6002
Entity Detected:	Ssbp2, single-stranded DNA binding protein 2 ( MGI:1914220)
Sequence:	sense strand is shown >T6002 CGGACGCGTGGGGCGTGGGTCGCCATGGGGACGGGGCTGTTCCCGGGGAGGCTGTGATGGGTTGACAGGT GCGTGACAGTGGGAGCTGCTCTCGGCACAAGCATGTACGGCAAAGGCAAGAGTAACAGCAGCGCCGTCCC GTCCGACAGCCAGGCCCGGGAGAAGTTAGCACTCTACGTGTATGAATATCTGCTCCATGTAGGAGCTCAG AAATCGGCTCAGACATTTTTATCAGAGATAAGATGGGAAAAAAACATCACATTGGGGGAGCCGCCCGGAT TCTTACATTCTTGGTGGTGTGTATTTTGGGATCTCTACTGTGCAGCTCCAGAGAGACGGGAAACCTGTGA ACACTCGAGCGAAGCTAAAGCCTTCCATGATTATCCTTTTATGTCGCCTCGGTACCCTGGCGGCCCGAGG CCCCCATTGAGGATACCTAACCAGGCACTTGGAGGTGTCCCAGGAAGTCAGCCATTACTCCCCAGTGGAA TGGATCCAACACGACAACAAGGACATCCAAATATGGGTGGACCAATGCAGAGAATGACTCCCCCAAGAGG AATGGTGCCCTTAGGACCACAGAACTATGGAGGTGCAATGAGACCCCCACTGAATGCTTTAGGTGGCCCT GGAATGCCTGGAATGAACATGGGTCCAGGTGGTGGTAGACCTTGGCCAAACCCAACAAATGCGAATTCAA TACCCTACTCCTCAGCGTCTCCTGGGAATTACGTAGGTCCTCCGGGAGGTGGAGGACCACCAGGAACACC CATCATGCCTAGTCCAGCAGATTCAACTAATTCTGGAGACAACATGTATACTTTAATGAATGCAGTACCT CCTGGGCCTAACAGACCTAATTTTCCAATGGGCCCTGGATCAGATGGTCCTATGGGTGGATTAGGAGGAA TGGAGTCCCATCACATGAACGGCTCTTTAGGCTCAGGAGATATGGACAGTATTTCCAAGAATTCTCCCAA TAATATGAGCCTGAGTAACCAGCCGGGCACTCCAAGGGATGACGGTGAAATGGGGGGAAATTTCTTAAAT CCTTTTCAGAGTGAGAGTTACTCCCCCAGCATGACCATGAGCGTGTGATCCGTTGCTGCGTCTCTCATGA AAACCATCGTGAGTCAGCCCATCACAGAACTACTACAGAAGAAACTACTCGTCGTGTACAGTTAGACAAA GGAATCTCAGACAACCAGACCACCTTTTAGTTCCTGCTCTCTCCCCTATTTTGAGAAGAAAGCGGGTCCA AATGTGATTCAAGCAACTGTACGGAGCGGCACATTAGCATTGCCCCCAACTGAACTGCGATGATTATCTG TGTGTATGTGTGGGAAGAGAATGCATCGTATATGCAGATCTGTATGCACATATACGCATACGTGCATTGA CCCACAGGACATTGTAAGATAGTATCACATGACATCTTAAGTAGAAGTAAGTAGGACTTTTATGCCATCC TTTTTTTCACGTTTACACTTTAATTATTAAAGGTTGCTCCTGCCCTTCCCCAACTATTTTGTGCTGTGTA CATCACTGCTTTATATAAGTTATTTTTTAAGGTGAACTCAGATGTTATGGTTTTGTAAATGTCTGCAATC ATGGATAGGAATAAAATTGCTTATTTGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3486988 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3486988 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002180 same experiment
	EMAGE:29860 same embryo
	EMAGE:29863 same embryo
	EMAGE:29887 same embryo
	EMAGE:32157 same embryo
	EMAGE:32137 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source